MOLECULAR ANALYIS OF A NEUROTROPIC AGENT, BORNA VIRUS

神经营养剂博尔纳病毒的分子分析

• 批准号: 3416240
• 负责人: W. Ian Lipkin
• 金额: $ 10.91万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-05-01 至 1994-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3416240
• 关键词: HIV infections; RNA virus; animal tissue; cell nucleus; human tissue; in situ hybridization; molecular biology; molecular cloning; nervous system disorder; neurotropic virus; virus RNA; virus characteristic; virus diseases; virus protein

Borna disease (BD) is an infectious neurologic disease characterized by profound behavioral disturbances and the accumulation of specific antigens in limbic system neurons. Though BD was originally described in horses, its host range includes birds, sheep and primates. Antibodies to BD antigens have been described in patients with bipolar depression and in patients with AIDS-encephalopathy. Thus, BD may be important not only as a model for virus-induced behavioral disorders but also because the BD agent may be a human pathogen. We have recently cloned cDNAs encoded by the BD agent and have used these cDNAs as probes to characterize the BD agent. Our data indicates that the BD agent is a single-strand, negative-sense RNA virus with a genome of 8.5 kb encoding two major mRNAs of 2.1 kb and 0.8 kb. Cell-fractionation experiments showed that the 2.1 kb and 0.8 kb RNAs are cytoplasmic; in contrast, the 8.5 kb RNA is nucleus-associated. This project will have two complementary goals; 1. to continue our studies in the molecular biology of the BD agent; 2. to use our molecular probes for the BD agent to ask whether BD can be implicated in selected human neuropsychiatric diseases.

伯纳病（BD）是一种感染性神经系统疾病，其特征是 深刻的行为干扰和特定抗原的积累 在边缘系统神经元中。 尽管BD最初是在马中描述的，但 它的宿主范围包括鸟类，绵羊和灵长类动物。 BD的抗体 在躁郁症抑郁症患者中已经描述了抗原 患有辅助 - 脑病的患者。 因此，BD可能不仅很重要 病毒引起的行为障碍的模型，也是因为BD 药物可能是人类病原体。 我们最近克隆了由编码的cDNA BD代理并已使用这些cDNA作为探针来表征BD 代理人。 我们的数据表明BD代理是单链， 负义RNA病毒，基因组为8.5 kb，编码两个主要mRNA 2.1 kb和0.8 kb。 细胞分级实验表明2.1 Kb和0.8 kb RNA是细胞质的；相反，8.5 kb的RNA为 核相关。 该项目将有两个互补的目标； 1 继续研究BD剂的分子生物学； 2。使用 BD剂询问BD是否可能与BD有关的分子探针 在选定的人类神经精神疾病中。