Center for Solutions for ME/CFS

ME/CFS 解决方案中心

基本信息

批准号：
9761452
负责人：
W. Ian Lipkin
金额：
$ 224.18万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-09-22 至 2022-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9761452
关键词：
Abbreviations Advisory Committees Algorithms Analytical Chemistry Autoantibodies Basic Science Biochemistry Biological Biological Assay Biological Markers Biology Blood specimen Caregivers Cells Centers for Disease Control and Prevention (U.S.)Chemical Structure Chronic Chronic Fatigue Syndrome Clinical Clinical Investigator Collection Communities Comorbidity Complex Consultations Crowding Data Data Scientist Databases Diagnosis Diagnostic Diagnostic Procedure Diagnostic tests Disease Edible Plants Emerging Communicable Diseases Ensure Epidemiologist Epidemiology Epigenetic Process Etiology Exercise Exercise Tolerance Exercise stress test Expression Profiling Family Fatigue Fibromyalgia Flavonoids Foundations Frequencies Funding Gene Expression Genetic Genomics High-Throughput Nucleotide Sequencing Immune Immune response Immunity Infection Infectious Disease Immunology Interdisciplinary Study International Intervention Intramural Research Program Investigation Investments Laboratories Laboratory Scientists Lead Lesion Libraries Link Lipids Literature Metabolic Pathway Methods Microbe Microbiology Mining Molecular Murine leukemia virus National Institute of Neurological Disorders and Stroke North America Oral Outpatients Pathogenesis Patient Care Patients Peptides Peripheral Blood Mononuclear Cell Phenotype Physicians Plasma Population Positioning Attribute Prevalence Procedures Proteomics RNA Reporting Research Research Personnel Research Project Grants Resources Risk Role Sampling Scientist Security Serological Source Standardization Study Subject Surveys Systems Biology Testing Thinness Translational Research United States National Institutes of Health Universities Viral Virus Vision Work base biodefense biomarker discovery cheminformatics clinical database cohort computerized data processing crowdsourcing data management density design education research evidence base flexibility functional genomics holistic approach insight instrument inter-institutional member metabolome metabolomics microbial microbiome mobile application polyphenol pressure prevent programs prospective repository response stressor tool transcriptome transcriptome sequencing transcriptomics

项目摘要

Overall Abstract The Center for Solutions for ME/CFS (CfS for ME/CFS) is an inter-disciplinary, inter-institutional center comprised of clinicians, clinical investigators, basic scientists who are committing to working together to understand the pathogenesis of ME/CFS and develop evidence-based strategies for interventions that prevent and mitigate disease. The team initially coalesced with an NIH call to respond to spurious reports linking xenotropic murine leukemia virus-related virus (XMRV) to ME/CFS. It consolidated its vision with support from the Hutchins Family Foundation Chronic Fatigue Initiative (CFI) and a crowd-funding organization, The Microbe Discovery Project, to explore the role of infection and immunity in disease and identify biomarkers for diagnosis through functional genomic, proteomic, and metabolomic discovery. The foundation for this project is a large clinical database and sample repository representing oral, fecal, and blood samples from well-characterized ME/CFS subjects and frequency-matched controls collected nationwide over a period of several years. In Project 1, we survey for the presence of molecular footprints of bacterial, fungal, and viral agents and corresponding immune responses in a 100 case/100 control subset of repository samples using high throughput sequencing, high density peptide arrays, and immune signature assays; this Project also examines the prevalence of autoantibodies selected based on clinical and literature reports. In Project 2, we profile the plasma metabolome and PBMC transcriptome in the same subjects studied in Project 1 using state-of-the-art mass spectrometric and RNA-seq methods, comprehensive mass spectral libraries, and tools for RNA profiling in bulk cell populations using cell sub-type specific markers. We also pursue metabolomic and transcriptomic analyses after orthostatic and exercise challenges using samples collected in Project 3. In Project 3, we mine existing databases at Columbia and the CFI for insights into clinical features, comorbidities, and sub-types that can be used to refine laboratory analyses, as well as enhance patient care. We will work with clinicians and the ME/CFS community to design a mobile app that will allow patients and caregivers to track clinical status in response to stressors and interventions, and will provide prospective research data. We will also investigate the utility of the Lean Test as a simple outpatient test for autonomic function.

全面的抽象的 ME/CFS 解决方案中心 (CfS for ME/CFS) 是一个跨学科、跨机构的中心由临床医生、临床研究人员、基础科学家组成，他们致力于共同努力了解 ME/CFS 的发病机制，并制定基于证据的干预策略，以预防并减轻疾病。该团队最初与美国国立卫生研究院联合呼吁回应虚假报告 ME/CFS 的异种鼠白血病病毒相关病毒 (XMRV)。在来自以下机构的支持下，它巩固了其愿景：哈钦斯家庭基金会慢性疲劳倡议 (CFI) 和众筹组织 The Microbe 发现项目，探索感染和免疫在疾病中的作用并确定用于诊断的生物标志物通过功能基因组学、蛋白质组学和代谢组学的发现。该项目的基础是一个大型临床数据库和样本库，代表来自充分表征的口腔、粪便和血液样本 ME/CFS 受试者和频率匹配对照在几年内在全国范围内收集。在项目 1，我们调查细菌、真菌和病毒因子的分子足迹的存在，并在 100 个病例/100 个储存库样本的对照子集中使用高高通量测序、高密度肽阵列和免疫特征测定；该项目还审查根据临床和文献报告选择的自身抗体的流行情况。在项目 2 中，我们分析了使用最先进的技术在项目 1 中研究的同一受试者中进行血浆代谢组和 PBMC 转录组质谱和 RNA-seq 方法、综合质谱库以及 RNA 分析工具使用细胞亚型特异性标记在大量细胞群中进行检测。我们还追求代谢组学和转录组学使用项目 3 中收集的样本进行直立和运动挑战后的分析。在项目 3 中，我们挖掘哥伦比亚大学和 CFI 的现有数据库，深入了解临床特征、合并症和亚型可用于完善实验室分析并加强患者护理。我们将与临床医生和 ME/CFS 社区设计一款移动应用程序，允许患者和护理人员跟踪临床状态对压力源和干预措施的反应，并将提供前瞻性研究数据。我们也会调查精益测试作为一种简单的自主神经功能门诊测试的实用性。