Cell Culture and Cell Phenotyping

细胞培养和细胞表型分析

• 批准号: 10438809
• 负责人: DANIELLE M. ROBERTSON
• 金额: $ 19.93万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-30 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10438809
• 关键词: Area; Calendar; Cell Culture Techniques; Cell Line; Cell Separation; Cells; Core Grant; Cornea; Cryopreservation; Data; Development; Endotoxins; Ensure; Equipment; Experimental Designs; Flow Cytometry; Fostering; Funding; Generations; Genetic Fingerprintings; Goals; Grant; Individual; Institution; Laboratories; Maintenance; Metabolic; Methods; Mycoplasma; Ophthalmology; Phenotype; Pilot Projects; Postdoctoral Fellow; Primary Cell Cultures; Records; Reproducibility; Research Activity; Research Personnel; Research Project Grants; Resources; Retina; Schedule; Services; Short Tandem Repeat; Students; Technical Expertise; Training; Vision; Vision research; arm; cell immortalization; design; high throughput screening; instrument; interest; investigator training; microvesicles; operation; programs; routine screening; testing services; vision science

Resource/Service Core: Cell Culture and Cell Phenotyping Module Abstract The goal of the Cell Culture and Cell Phenotyping Module is to streamline cell culture and phenotyping analyses for vision researchers. To accomplish this goal, this Module will support the maintenance and authentication of cell lines that are required for rigorous study of a wide range of NEI-focused research activities, including all NEI-funded research projects and new collaborative and pilot projects. The director and staff of this Module will provide training, technical support, and maintenance of key equipment to facilitate scientifically rigorous, cutting edge, vision research. The Cell Culture arm of this module will provide the following essential services: 1) Routine maintenance of immortalized cell lines. 2) Maintenance of cryopreserved stocks of cell lines. 3) Establishment of primary corneal and retinal cell lines. 4) Routine screening for mycoplasma and endotoxin contamination. 5) Short tandem repeat DNA profiling for cell line authentication. The Cell Phenotyping arm of this module will provide the following comprehensive analytical services: 1) Flow cytometry. 2) Cell sorting. 3) High throughput cellular analysis. 4) Live cell metabolic profiling. 5) Microvesicle quantification and size determination. Collectively, these services will provide all NEI-funded vision researchers on campus with state of the art resources that will increase their experimental efficiency and support the generation of high quality, quantitative data for all aspects of vision research.

资源/服务核心：细胞培养和细胞表型分析模块 抽象的 细胞培养和细胞表型分析模块的目标是简化细胞培养和表型分析 为视觉研究人员进行分析。为了实现这一目标，该模块将支持维护和 对以 NEI 为重点的广泛研究进行严格研究所需的细胞系的验证 活动，包括所有 NEI 资助的研究项目以及新的合作和试点项目。导演和 该模块的工作人员将提供培训、技术支持和关键设备的维护，以方便 科学严谨、前沿、愿景的研究。 该模块的细胞培养部门将提供以下基本服务： 1）永生化细胞系的日常维护。 2) 细胞系冷冻储存的维护。 3)原代角膜和视网膜细胞系的建立。 4）支原体、内毒素污染常规筛查。 5) 用于细胞系认证的短串联重复 DNA 分析。 该模块的细胞表型分析部门将提供以下全面的分析服务： 1）流式细胞仪。 2）细胞分选。 3) 高通量细胞分析。 4) 活细胞代谢分析。 5)微泡定量和尺寸测定。 总的来说，这些服务将为校园内所有 NEI 资助的视觉研究人员提供最先进的服务 资源，将提高他们的实验效率并支持生成高质量、定量的 视觉研究各个方面的数据。