Virology and Technology Core

病毒学和技术核心

基本信息

批准号：
7994928
负责人：
Michael Allen Brehm
金额：
$ 18.34万
依托单位：
UNIV OF MASSACHUSETTS MED SCH WORCESTER
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-06-01 至 2015-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7994928
关键词：
Agonist Alloantigen Allogenic Animal Model Animals Antigens Antiviral Agents Area Beds Biological Assay CTL assay Calendar Caring Cell Count Cellular Assay Clinic Data Analyses Detection Ensure Epitopes Evaluation Graft Survival Growth Hemorrhage Human Resources Immune response Immunobiology Incubators Individual Infection Infectious Agent Laboratories Libraries MHC Class I Genes Measures Medicine Memory Methodology Monitor Mus Natural Killer Cells Peptides Pharmaceutical Preparations Preparation Program Research Project Grants Protocols documentation Reagent Research Personnel Schedule Scientist Services T-Cell Proliferation T-Lymphocyte Techniques Technology Time Tissue Harvesting Tissues Training Transplantation Viral Virus Virus Diseases Work cytokine cytotoxicity design dimer experience feeding human disease in vivo insight meetings new technology novel programs research study tissue culture virology

项目摘要

The purpose ofthe Virology and Technology Core is two fold: 1) to provide facilities and technical assistance for the conduction of virus infections and assays in mice, and 2) to facilitate the use of novel assays that we have developed by each ofthe Projects to examine immune responses against alloantigens. One room within a Biosafety level two/three (BSL2/3) Biocontainment Suite will be assigned for work with viruses, for their subsequent surveillance and monitoring, and for the bleeding of mice and harvesting of tissues. For viral and immunological assays, I have a BSL2 laboratory area that has a separate attached tissue culture room equipped with incubators and laminar flow hoods for tissue culture work. This Core will be responsible for the growth, preparation, purification, and assay of viruses, maintaining stocks of peptide epitopes, and for the preparation of antigen-specific MHC-multimer reagents (MHC-lg-dimers and MHCtetramers). The Core will provide reagents and training for assays to quantify and characterize virus-specific T cells including the use of MHC-multimers, T cell proliferation assays, intracellular cytokine assays, and antiviral CTL assays. The new technology to be used include in vivo cytotoxicity assays to measure NK and T cell activity against allogeneic tissues, and intracellular cytokine assays to examine naive, effector/memory, and tolerized alloreactive T cells. The services to be provided by the Core will be available to all Project Laboratories, and the Core will also be responsible for training personnel to peri'orm assays

病毒学和技术核心的目的有两个：1）提供设施和技术协助在小鼠中进行病毒感染和测定，以及 2) 促进新型药物的使用我们通过每个项目开发的检测方法来检查针对同种抗原的免疫反应。生物安全二级/三级 (BSL2/3) 生物防护套件内的一间房间将被分配用于与病毒，用于随后的监视和监测，以及用于小鼠放血和收获组织。对于病毒和免疫学检测，我有一个 BSL2 实验室区域，该区域有一个单独的附加设备组织培养室配备培养箱和层流罩，用于组织培养工作。该核心将负责病毒的生长、制备、纯化和测定，维持肽库存表位，以及用于制备抗原特异性 MHC 多聚体试剂（MHC-lg-二聚体和 MHC 四聚体）。该核心将为量化和表征病毒特异性的检测提供试剂和培训 T 细胞，包括使用 MHC 多聚体、T 细胞增殖测定、细胞内细胞因子测定和抗病毒CTL测定。将使用的新技术包括体内细胞毒性测定，以测量 NK 和针对同种异体组织的 T 细胞活性，以及细胞内细胞因子测定以检查初始、效应/记忆和耐受同种反应性 T 细胞。核心提供的服务将可用到所有项目实验室，核心还将负责培训人员进行期间检测