Telemetric Regenerative Bandage for Accelerating Wound Healing

用于加速伤口愈合的遥测再生绷带

基本信息

批准号：
10346507
负责人：
Guillermo Antonio Ameer
金额：
$ 64.9万
依托单位：
NORTHWESTERN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-24 至 2026-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10346507
关键词：
Acrylamides Address Adhesions Amputation Animal Model Animals Antioxidants Attenuated Bacterial Infections Bandage Biocompatible Materials Blood Circulation Body Temperature Caring Cells Chronic Citrates Clinic Clinical Complications of Diabetes Mellitus Copper Dermal Development Devices Diabetes Mellitus Diabetic Foot Ulcer Diabetic mouse Digit structure Disease Endothelial Cells Engineering Exposure to Family suidae Feedback Fibroblasts Free Radicals Gel Goals Health Healthcare High Prevalence Hospitals Human Immobilization Impaired healing Impaired wound healing Impairment Implant In Vitro Incidence Infection Inflammation Laminin Lead Left Limb structure Location Lower Extremity Measures Medical Device Metabolic syndrome Metals Miniature Swine Monitor Operative Surgical Procedures Oxidative Stress Patient-Focused Outcomes Patients Peptides Personal Satisfaction Physicians Polyethylene Glycols Population Positioning Attribute Prevalence Process Property Research Research Personnel Safety Sepsis Shapes Skin Skin wound healing Societies Specific qualifier value Sterile coverings Stretching System Telemetry Temperature Testing Thick Time Tissues Vascularization Wireless Technology Work Wound Infection angiogenesis base biomaterial compatibility blood perfusion clinically relevant db/db mouse diabetes amputation diabetic diabetic ulcer effective therapy flexibility healing health care delivery health care disparity human disease improved innovation keratinocyte limb amputation macromolecule microsensor migration mouse model nanoparticle nanoparticle delivery neovascularization non-healing wounds novel outcome prediction porcine model prevent regenerative research clinical testing scaffold sensor skin wound therapy outcome tissue regeneration tool wound wound bed wound care wound closure wound dressing wound healing

项目摘要

Summary Diabetic foot ulcers (DFUs) are a major complication of diabetes. These sores, if left untreated, can become infected and become a serious threat to the patient’s well being. Although the field of wound care management is well established, the effective treatment of chronic DFUs remains a challenge. The primary goal in the treatment of DFUs is for the wound to close as soon as possible and to do so in a durable way. However, prolonged inflammation, oxidative tissue damage, and impaired blood circulation in diabetic wounds delay the wound healing process, resulting in open, non-healing wounds that often lead to limb amputations. This proposal will address these problems by developing a versatile wound dressing that restores normal wound healing rates by reducing free radicals in the wound, providing a native-like scaffold for the cells to divide and migrate, and enhancing vascularization in the wound. Another problem is the inability to monitor the wound in real time after the patient leaves the hospital, leading to digit or limb amputations. We will address this problem by developing a wireless system that can monitor the temperature and pH of the wound in real time, parameters that have been shown to be indicators of infection. Therefore, the overall goal of this proposal is to develop a shape- conforming antioxidant dressing that upon exposure to body temperature transforms into a gel that promotes new tissue formation in diabetic wounds and a feedback system that involves tissue conforming sensors to monitor bacterial infection and/or lack of healing. Toward this goal, we have developed a novel macromolecule - poly (polyethylene glycol citrate-co-N isopropyl acrylamide) - that incorporates a laminin-derived peptide. This material, referred to as PPCN-A5G81, supports tissue regeneration and can conform to the unique shape and depth of a wound. As for wireless monitoring of the wound, we pioneered the development of flexible, stretchable electronic sensors that can be integrated with human skin or implanted into the body for continuous, non-invasive health monitoring and treatment of disease. We hypothesize that: 1) incorporating immobilized Cu2+ into PPCN-A5G81 will confer vasculoinductive properties that significantly increase PPCN-A5G81’s ability to restore normal healing rates of full thickness dermal wounds in diabetic mouse and swine models; and 2) conforming temperature and pH sensors are safe and can remotely provide real time information regarding blood perfusion and infection in dermal wounds in diabetic animals. The specific aims of this proposal are to: 1) fabricate a PPCN-based regenerative dressing with vasculoinductive, dermoconductive, and dermoinductive properties and investigate its safety and efficacy for healing full thickness wounds in diabetic mice and diabetic pigs with metabolic syndrome; and 2) fabricate and characterize telemetric wound feedback tissue-conforming sensors capable of measuring temperature and pH in infected and non-infected diabetic dermal wounds. Results from this research will contribute to the development of innovative clinical products that reduce amputation rates and improve patient outcome.

概括糖尿病足溃疡 (DFU) 是糖尿病的主要并发症，如果不及时治疗，这些溃疡可能会发展为糖尿病足溃疡。尽管在伤口护理管理领域，感染并严重威胁患者的健康。尽管已得到充分证实，但慢性 DFU 的有效治疗仍然是该领域的主要目标。 DFU 的治疗是为了让伤口尽快闭合，并且以持久的方式闭合。糖尿病伤口的长期炎症、氧化组织损伤和血液循环受损会延迟伤口愈合过程中，导致开放性、不愈合的伤口，常常导致肢体截肢。将通过开发一种多功能伤口敷料来解决这些问题，该敷料可以恢复正常的伤口愈合率通过减少伤口中的自由基，为细胞分裂和迁移提供类似天然的支架，以及增强伤口血管化的另一个问题是术后无法实时监测伤口。患者离开医院，导致手指或肢体截肢，我们将通过开发来解决这个问题。无线系统，可以实时监测伤口的温度和pH值，参数已被因此，该提案的总体目标是开发一种形状- 顺应性抗氧化敷料，在暴露于体温时会转化为凝胶，促进糖尿病伤口中新组织的形成以及涉及组织的反馈系统为了实现这一目标，我们拥有用于监测细菌感染和/或愈合不良的传感器。开发了一种新型大分子——聚（聚乙二醇柠檬酸酯-co-N异丙基丙烯酰胺）—— 这种材料被称为 PPCN-A5G81，含有层粘连蛋白衍生肽，支持组织再生。并且可以符合伤口的独特形状和深度。对于伤口的无线监测，我们。率先开发了灵活、可拉伸的电子传感器，可以与人体皮肤或植入体内进行持续、非侵入性的健康监测和疾病治疗。 1) 将固定化 Cu2+ 掺入 PPCN-A5G81 将赋予血管诱导特性，显着提高 PPCN-A5G81 恢复糖尿病小鼠全层真皮伤口正常愈合率的能力和猪模型；2) 符合标准的温度和 pH 传感器是安全的，可以远程提供实时信息有关糖尿病动物皮肤伤口的血液灌注和感染的信息。该提案旨在：1）制造一种基于 PPCN 的再生敷料，具有血管诱导性、皮肤传导性、和皮肤感应特性，并研究其治疗糖尿病全层伤口的安全性和有效性患有代谢综合征的小鼠和糖尿病猪；2) 构建并表征遥测伤口反馈组织顺应性传感器能够测量感染和未感染糖尿病患者的温度和 pH 值这项研究的结果将有助于开发创新的临床产品。降低截肢率并改善患者的治疗效果。