LYMPHOID CELLS PRODUCTION OF ANTIBODIES

淋巴细胞产生抗体

基本信息

批准号：
3115516
负责人：
G. J THORBECKE
金额：
$ 12.22万
依托单位：
NEW YORK UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1984
资助国家：
美国
起止时间：
1984-04-01 至 1992-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3115516
关键词：
B lymphocyte T lymphocyte antibody antigens cell differentiation chickens complement delayed hypersensitivity graft versus host disease histocompatibility immune tolerance /unresponsiveness immunity immunochemistry immunologic memory immunoregulation laboratory mouse lymphatic tissue

项目摘要

The function and fate of germinal center cells from lymphoid tissues will be analyzed in both mice and chickens. Particular attention will be given to kinetic events and their correlation to functional changes in B memory cells. Localization of radioactively labeled primed and unprimed B cells, and the influence of localized specific antigen on isolated primed B cell localization in recipients, will be followed both morphologically and functionally. Induction of B cell memory by injection of anti-idiotype alone or in the form of immune complex with idiotype will be tried in A/J mice, using the model of the major idiotype in the response to ARS in this strain. Athymic mice of A/J background will also be included. If such priming is obtained, the histological counterpart will be studied with respect to germinal center generation. Studies on acute antiidiotype formation during the response of mice to TNP on polysaccharide carriers are being continued with emphasis on its role in tolerance induction and on the role of idiotype specific T cells in this phenomenon. Experiments on B cell receptor blockade with TNP-polysaccharide antigens are being conducted in order to analyze the reversibility of such blockade procedures in terms of remaining responsiveness of the cells. The influence of infection with vesicular stomatitis virus on the induction of suppressor T cells as compared to helper T cells in vivo will be analyzed. Finally, the epidermal Langerhans cell is being studied and compared functionally to interdigitating reticulum cells of lymphoid tissue.

淋巴组织生发中心细胞的功能和命运将是在小鼠和鸡中进行了分析。将特别关注动力学事件及其与B记忆细胞中功能变化的相关性。放射性标记的底漆和未填充的B细胞的定位，以及局部特异性抗原对分离的引发B细胞定位的影响在接受者中，将在形态和功能上遵循。单独或以抗IDIOTYPE的注射或以使用白痴型的免疫复合物将在A/J小鼠中尝试，使用该模型在这种菌株中对ARS的响应中的主要白痴。 A/J的胸骨小鼠背景也将包括在内。如果获得了这种启动，则组织学将研究对应方有关生发中心的生成。研究在小鼠对TNP响应期间的急性抗替代型形成上多糖载体正在继续，重点是其在耐受性诱导以及特定型T细胞在此中的作用现象。用TNP-碳糖糖阻断B细胞受体阻断的实验正在进行抗原，以分析这种可逆性封锁程序是在细胞的剩余反应性方面进行的。这囊泡气孔病毒感染对诱导的影响将分析与体内辅助T细胞相比的抑制剂T细胞。最后，正在研究并比较表皮Langerhans细胞在功能上与淋巴组织的网状细胞相互作用。