Swine Core - Regional/Shared Resource Core

养猪核心 - 区域/共享资源核心

• 批准号: 9105742
• 负责人: Michael Sturek
• 金额: $ 17.16万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9105742
• 关键词: Age; Animal Model; Arteries; Atherogenic Diet; Atherosclerosis; Biotechnology; Blood Flow Velocity; Body Composition; Breeding; Cardiovascular Diseases; Catheters; Cell physiology; Cholesterol; Clinical Medicine; Collaborations; Complex; Control Groups; Databases; Development; Diabetes Mellitus; Diet; Dyslipidemias; Endocrine; Environment; Euglycemic Clamping; Family suidae; Fasting; Funding; Glucose; Glucose Clamp; Health; High Density Lipoproteins; Human; Hypertension; Image; In Vitro; Indiana; Institutes; Insulin Resistance; Intervention; Intravenous; Kidney Calculi; Lipids; Lithotripsy; Liver; Low-Density Lipoproteins; Maintenance; Measures; Medicine; Metabolic; Metabolic syndrome; Metabolism; Methods; Microscopy; Microvascular Dysfunction; Miniature Swine; Modeling; Molecular; Monitor; Morphology; National Heart, Lung, and Blood Institute; National Institute of Diabetes and Digestive and Kidney Diseases; Non-Insulin-Dependent Diabetes Mellitus; Oral; Pathogenesis; Phenotype; Positron-Emission Tomography; Quality Control; Quality of Care; Records; Research; Research Personnel; Resolution; Resource Sharing; Resources; Rodent; Severities; Skeletal Muscle; Stents; Telemetry; Testing; Therapeutic; Tissue Banking; Tissue Banks; Tissues; Transgenic Mice; Translational Research; Translations; Triglycerides; Ultrasonography; United States National Institutes of Health; Universities; Vaccination; X-Ray Computed Tomography; abstracting; animal resource; bariatric surgery; comparative; cost efficient; impaired glucose tolerance; in vivo; indexing; islet; medical schools; metabolic abnormality assessment; mouse model; pressure; research study; sample fixation

Abstract (Swine Core) The central function of the Indiana Diabetes Research Center (IDRC) Swine Core (a Regional/Shared Resource Core) is to reduce barriers to the use of Ossabaw swine, a unique animal model that closely approximates human metabolic syndrome (MetS) and progression to type 2 diabetes. Providing this animal resource enables testing of numerous hypotheses about the integrated, in vivo pathogenesis and long-term complications of MetS and type 2 diabetes and provides tissues for studies of cellular and molecular mechanisms. The Comparative Medicine Center, a collaboration between Purdue University and Indiana University School of Medicine (lUSM), has the only research and large-scale breeding colony of Ossabaw miniature swine in the world. Induced by an excess calorie atherogenic diet, Ossabaw swine develop MetS and atherosclerosis among other complications, and are an ideal model for testing human therapies. The Ossabaw swine resource has been used for numerous studies, including metabolism in skeletal muscle and liver, percutaneous catheter interventions and stents, lithotripsy of kidney stones, and bariatric surgery among others. The current and projected demand for Ossabaw miniature swine for NIDDK and several NIH Institutes, universities and biomedical and biotechnology companies clearly states the need for this animal model and justifies this Core as a Regional/National Shared Resource. The Swine Core is a critical interface in the translation of research from simpler animal models (Rodent Core) to humans (Translation Core). Islet and Microscopy Cores will be complementary with the Swine Core for basic cellular characterization. The primary aims of the Swine Core are to (1) provide quality control and care of the breeding colony of Ossabaw swine, (2) provide quality control of diet-induced MetS and diabetes (MetS/D), (3) phenotype the endocrine, metabolic, and dyslipidemia indices of MetS/D, (4) phenotype cardiovascular disease, and (5) provide a tissue and data bank for distribution for research studies. These aims of the Swine Core will reduce barriers to study of this highly relevant animal model, and will facilitate the progressive translation of research from simpler, less complex models to humans.

摘要（猪核心） 印第安纳州糖尿病研究中心 (IDRC) 养猪核心（区域/共享 资源核心）旨在减少使用 Ossabaw 猪的障碍，Ossabaw 猪是一种独特的动物模型，与 近似人类代谢综合征 (MetS) 并进展为 2 型糖尿病。提供这种动物 资源能够测试关于综合、体内发病机制和长期的多种假设 MetS 和 2 型糖尿病的并发症，并为细胞和分子研究提供组织 机制。普渡大学和印第安纳州合作的比较医学中心 大学医学院（lUSM），拥有奥萨博唯一的研究和大规模繁殖群体 世界上的小型猪。由于热量过多、致动脉粥样硬化饮食，Ossabaw 猪会出现 MetS 和 动脉粥样硬化等并发症，是测试人类疗法的理想模型。奥萨博 猪资源已用于许多研究，包括骨骼肌和肝脏的新陈代谢， 经皮导管介入治疗和支架、肾结石碎石术以及减肥手术 其他的。 NIDDK 和几个 NIH 研究所对 Ossabaw 小型猪的当前和预计需求， 大学和生物医学和生物技术公司明确表示需要这种动物模型，并且 证明该核心作为区域/国家共享资源的合理性。 Swine Core 是一个关键接口 将研究从简单的动物模型（啮齿类核心）转化为人类（翻译核心）。胰岛和 显微镜核心将与猪核心互补，以进行基本的细胞表征。初级 养猪核心的目标是 (1) 提供奥萨巴猪种猪群的质量控制和护理， (2) 提供饮食引起的 MetS 和糖尿病 (MetS/D) 的质量控制， (3) MetS/D的内分泌、代谢和血脂异常指数表型， (4)表型心血管疾病，以及 (5) 提供组织和数据库供研究分发。 猪核心的这些目标将减少研究这种高度相关的动物模型的障碍，并将 促进研究从更简单、不太复杂的模型逐步转化为人类。