Swine Core - Regional/National Shared Resources Core

养猪核心 - 区域/国家共享资源核心

基本信息

批准号：
10155472
负责人：
Michael Sturek
金额：
$ 24.61万
依托单位：
INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-07-06 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10155472
关键词：
Animal Model Atherogenic Diet Biotechnology Body Composition Breeding Calories Catheters Chemicals Cholesterol Clinical Medicine Collaborations Complex Control Groups Corvus Diabetes Mellitus Diet Dyslipidemias Endocrine Family suidae Fasting Funding Glucose Glucose Clamp Health High Density Lipoproteins Human Implant Indiana Informatics Insulin Resistance Intervention Intravenous Kidney Calculi Licensing Lipids Lithotripsy Liver Low-Density Lipoproteins Medicine Metabolic Metabolic syndrome Metabolism Methods Microscopy Miniature Swine Modeling Molecular Monitor National Heart, Lung, and Blood Institute National Institute of Diabetes and Digestive and Kidney Diseases Non-Insulin-Dependent Diabetes Mellitus Oral Pathogenesis Phenotype Physiology Positron-Emission Tomography Production Research Research Personnel Resource Sharing Resources Rodent Services Severities Skeletal Muscle Stents System Testing Therapeutic Thinness Tissue Banks Tissues Transgenic Mice Translational Research Translations Triglycerides Ultrasonography Universities X-Ray Computed Tomography bariatric surgery comorbidity comparative cost effectiveness cost efficient data repository diabetic human disease human model imaging study impaired glucose tolerance improved in vivo indexing islet medical schools metabolic abnormality assessment mouse model operation ranpirnase translation to humans

项目摘要

ABSTRACT (SWINE CORE) The Swine Core of the Indiana Diabetes Research Center is a Regional/National Shared Resource Core that has been operating and servicing researchers since 2004. The central function of the Swine Core is to reduce barriers to the use of Ossabaw swine, a unique animal model that closely approximates human metabolic syndrome (MetS) and progression to type 2 diabetes, with attendant long-term complications. Understanding and therapeutic treatment of MetS and diabetes with numerous long-term health complications in humans has been stifled by the lack of translational animal models. Experimental methods and translation to human clinical medicine is not possible from widely used rodent and transgenic mouse models. The Swine Core will characterize MetS and progression to type 2 diabetes and/or chemically-induced diabetes (MetS/D) and the resulting comorbidities in Ossabaw swine. We have provided Ossabaw tissue and/or live pigs to ~160 investigators during the 15 years of the Swine Core operation. A bank of ~50 different tissues available from each pig has enabled widespread dissemination to other investigators, providing outstanding resource sharing and cost-effectiveness. Providing this swine resource enables testing of numerous hypotheses about the integrated, in vivo pathogenesis and long-term complications and provides tissues for studies of cellular and molecular mechanisms. The Swine Core is a critical interface in the translation of research from simpler animal models (Islet & Physiology Core) to humans (Translation Core). The Swine Core is Directed by Dr. M. Sturek, a leader in modeling human diseases in swine, and Dr. M. Alloosh serves as the Associate Director. The Specific Aims of the Swine Core are to: (1). Make lean and diet-induced MetS and diabetic (MetS/D) pigs readily available, i.e. “on the shelf”. (2). Determine the endocrine, metabolic, and dyslipidemia indices of MetS/D to screen for optimal phenotypes. (3). Provide a tissue and data bank for distribution. These aims of the Swine Core will reduce barriers to study of this highly relevant animal model, and will facilitate the progressive translation of research from simpler, less complex models to humans.

摘要（猪核心）印第安纳州糖尿病研究中心的猪核心是一个区域/国家共享资源核心，自 2004 年以来一直在为研究人员提供服务。猪核心的核心功能是减少使用奥萨巴猪的障碍，这是一种非常接近人类代谢的独特动物模型综合征 (MetS) 并进展为 2 型糖尿病，并伴有长期并发症。代谢综合征和糖尿病以及人类许多长期健康并发症的治疗和治疗由于缺乏转化动物模型和转化为人类临床而受到抑制。广泛使用的啮齿动物和转基因小鼠模型不可能提供药物。描述 MetS 的特征以及进展为 2 型糖尿病和/或化学诱导的糖尿病 (MetS/D) 以及我们已向约 160 头猪提供了 Ossabaw 组织和/或生猪。猪核心运营 15 年期间的研究人员提供的约 50 种不同组织的库。每头猪都能够向其他研究人员广泛传播，提供出色的资源共享提供这种猪资源可以测试有关猪的许多假设。整合的体内发病机制和长期并发症，并为细胞和细胞研究提供组织猪核心是从简单动物身上进行研究转化的关键接口。模型（胰岛和生理学核心）到人类（翻译核心）由 M. Sturek 博士指导， M. Allosh 博士是猪类人类疾病建模方面的领导者，M. Alloosh 博士担任副主任。养猪核心的具体目标是： (1). 使瘦肉型和饮食诱导的 MetS 和糖尿病 (MetS/D) 猪随时可用，即“上架”。 (2).确定MetS/D的内分泌、代谢和血脂异常指标以筛选最佳指标。表型。 (3) 提供组织和数据库以供分发。猪核心的这些目标将减少研究这种高度相关的动物模型的障碍，并将促进研究从更简单、不太复杂的模型逐步转化为人类。