FUNCTIONAL NEUROANATOMICAL BASIS OF SALT APPETITE

盐食欲的功能神经解剖学基础

• 批准号: 6243040
• 负责人: JOSEPH G VERBALIS
• 金额: $ 20.78万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 1998-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6243040
• 关键词: atrial natriuretic peptide; brain mapping; central neural pathway /tract; furosemide; gene expression; immunocytochemistry; in situ hybridization; laboratory rat; neuroendocrine system; nutrition related tag; oxytocin; phenotype; regulatory gene; salt intake

Salt appetite is a complex behavior that occurs under a variety of conditions, many of which are appropriate responses to real or perceived deficits of body sodium but some of which are not. As with other behaviors, interactions between peripheral and brain mechanisms are responsible for the presence, or absence, of salt appetite. In the brain it is now apparent that both excitatory and inhibitory components influence the expression of salt appetite. Studies from many laboratories have implicated several neuropeptides and several areas of the brain as potentially being involved in the control of salt appetite, but to date there is no consensus about the brain pathways essential for either excitation or inhibition of this behavior. Recent studies from our laboratories have shown that neuronal expression of the immediate early gene product Fos can identify neural pathways that are activated by a specific stimulus, and can differentiate these from pathways activated by related but nonetheless distinct stimuli. This methodology therefore enables an assessment of the brain circuits that are activated by stimuli that excite or inhibit specific behaviors under defined experimental conditions. The studies in this project utilize fos expression as a marker of neuronal activation after treatments known to excite or inhibit salt appetite in rats. Analysis of the stimulated patterns of Fos activation in response to multiple different treatments will allow identification of a common subset of brain areas that are activated in response to excitation or inhibition of salt appetite. Additional studies will pair excitatory and inhibitory treatments to ascertain the patterns of brain Fos activation under conditions of mixed stimuli that likely occur under physiological circumstances, and will compare the patterns of brain Fos activation in response to treatments that inhibit salt appetite to those accompanying physiological satiation of this appetite. Analysis of the neurochemical phenotypes and connectivity of the neurons activated to express Fos in response to treatments that either excite or inhibit salt appetite will allow analysis of the brain pathways involved in its control. These studies will therefore produce detailed descriptions of the brain areas that are essential for both excitation and inhibition of salt appetite in the rat, thereby defining the functional neuroanatomical basis of this important homeostatic behavior.

盐食欲是一种复杂的行为，发生在多种 条件，其中许多是对真实或 人体钠的缺陷，但其中一些不适。作为 与其他行为，外围和大脑之间的相互作用 机制是盐的存在或不存在的原因 食欲。在大脑中，现在显然是兴奋和 抑制性成分影响盐食欲的表达。 许多实验室的研究牵涉到几个 神经肽和大脑的多个区域可能存在 涉及盐食欲的控制，但迄今为止没有 关于任何激发必不可少的大脑通路的共识 或抑制这种行为。我们最近的研究 实验室表明，直接的神经元表达 早期基因产品FOS可以识别神经途径 被特定刺激激活，可以区分 途径被相关但不同的刺激激活。 因此，该方法可以评估大脑 激发或抑制的刺激激活的电路 在定义的实验条件下的特定行为。这 该项目的研究利用FOS表达作为标记 已知激发或抑制的治疗后神经元激活 大鼠的盐食欲。分析FOS的刺激模式 响应多种不同治疗的激活将允许 识别大脑区域的共同子集 响应激发或抑制盐而激活 食欲。其他研究将配对兴奋性和抑制性 治疗以确定大脑FOS激活的模式 生理下可能发生的混合刺激条件 情况，并将比较大脑FOS的模式 响应抑制盐食欲的治疗方法激活 伴随着这种食欲的生理饱足的人。 分析神经化学表型和连通性 神经元激活以表达FOS，以响应治疗 激发或抑制盐的食欲将允许分析 大脑通路涉及其控制。这些研究会 因此，对大脑区域的详细描述 对于盐食欲的激发和抑制至关重要 在大鼠中，从而定义了功能性神经解剖学基础 这种重要的体内稳态行为。