Microsurgery Core

显微外科核心

• 批准号: 10625535
• 负责人: Wenjun Li
• 金额: $ 23.3万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-05-12 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10625535
• 关键词: Microsurgery; Core

PROJECT SUMMARY/ABSTRACT Microsurgical models of murine organ transplantation have played a critical role in advancing our understanding of immunological pathways that regulate graft rejection and tolerance. Such models are important tools to develop therapies for transplant patients. As substantial differences exist between immune responses to various grafts, insights gained in one organ cannot be translated to other organs. Our laboratories have developed clinically relevant models of lung transplantation and re-transplantation in the mouse as well as novel approaches to image immune cell interactions in the murine pulmonary graft in real time. We and others have used these approaches to investigate clinically relevant lung-specific alloimmune responses. A dedicated microsurgery core was established at the initiation of this program project in 2015. The microsurgery core has proven instrumental in facilitating the execution of the experiments. In aim 1 we will continue to standardize the quality of microsurgical and imaging procedures for all three projects in the proposal. In aim 2 we will continue to standardize assessment of graft function and tissue harvest for all three projects. In aim 3 we will maintain a system that provides unfettered access to microsurgical transplants and coordinates intravital imaging procedures for Projects 1 and 3.

项目摘要/摘要 鼠器官移植的显微外科模型在推进我们的 了解调节移植排斥和耐受性的免疫学途径。这样的 模型是开发移植患者疗法的重要工具。作为实质性差异 存在于对各种移植物的免疫反应之间，在一个器官中获得的见解不可能是 翻译成其他器官。我们的实验室已经开发了肺部相关的肺模型 在小鼠中的移植和重新移植以及新颖的图像免疫方法 实时的鼠肺移植物中的细胞相互作用。我们和其他人使用了这些 研究临床上相关的肺特异性同应免疫反应的方法。专用 显微手术核心是在2015年该计划项目的启动时建立的。 显微手术核心已证明有助于促进实验的执行。目标 1我们将继续标准化这三个的微型外科和成像程序的质量 提案中的项目。在AIM 2中，我们将继续标准化对移植功能的评估和 所有三个项目的组织收获。在AIM 3中，我们将维护一个提供不受限制的系统 访问项目1 和3。