Microsurgery Core

显微外科核心

基本信息

批准号：
10197015
负责人：
Wenjun Li
金额：
$ 23.56万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-05-12 至 2025-05-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10197015
关键词：
Acute Address Biology Blood Vessels Cell Communication Chronic Data Ensure Event Flow Cytometry Functional disorder Funding Gene Expression Profiling Goals Graft Rejection Graft Tolerance Histology Human Image Imaging Techniques Immune Immune response Immunologics Immunology Laboratories Lead Left Lung Lung Transplantation Microsurgery Minor Modeling Mus Organ Organ Transplantation Outcome Pathologic Pathway interactions Physiological Play Preparation Procedures Protocols documentation Reperfusion Injury Reproducibility Respiratory Failure Role Serum Solid Specimen Standardization System Techniques Time Tissue Harvesting Tissues Training Translating Transplant Recipients Transplantation Transplantation Tolerance Universities Variant Virginia Washington antibody-mediated rejection clinically relevant experimental study graft function insight intravital imaging lung allograft mouse model novel strategies programs response therapy development tool transplant model two photon microscopy

项目摘要

PROJECT SUMMARY/ABSTRACT Microsurgical models of murine organ transplantation have played a critical role in advancing our understanding of immunological pathways that regulate graft rejection and tolerance. Such models are important tools to develop therapies for transplant patients. As substantial differences exist between immune responses to various grafts, insights gained in one organ cannot be translated to other organs. Our laboratories have developed clinically relevant models of lung transplantation and re-transplantation in the mouse as well as novel approaches to image immune cell interactions in the murine pulmonary graft in real time. We and others have used these approaches to investigate clinically relevant lung-specific alloimmune responses. A dedicated microsurgery core was established at the initiation of this program project in 2015. The microsurgery core has proven instrumental in facilitating the execution of the experiments. In aim 1 we will continue to standardize the quality of microsurgical and imaging procedures for all three projects in the proposal. In aim 2 we will continue to standardize assessment of graft function and tissue harvest for all three projects. In aim 3 we will maintain a system that provides unfettered access to microsurgical transplants and coordinates intravital imaging procedures for Projects 1 and 3.

项目摘要/摘要鼠器官移植的显微外科模型在推进我们的了解调节移植排斥和耐受性的免疫学途径。这样的模型是开发移植患者疗法的重要工具。作为实质性差异存在于对各种移植物的免疫反应之间，在一个器官中获得的见解不可能是翻译成其他器官。我们的实验室已经开发了肺部相关的肺模型在小鼠中的移植和重新移植以及新颖的图像免疫方法实时的鼠肺移植物中的细胞相互作用。我们和其他人使用了这些研究临床上相关的肺特异性同应免疫反应的方法。专用显微手术核心是在2015年该计划项目的启动时建立的。显微手术核心已证明有助于促进实验的执行。目标 1我们将继续标准化这三个的微型外科和成像程序的质量提案中的项目。在AIM 2中，我们将继续标准化对移植功能的评估和所有三个项目的组织收获。在AIM 3中，我们将维护一个提供不受限制的系统访问项目1 和3。