Leveraging spatial expression profiling for targeted manipulation of age-related proteins in the normal and Alzheimers disease brains

利用空间表达谱对正常和阿尔茨海默病大脑中的年龄相关蛋白进行靶向操作

• 批准号: 10913080
• 负责人: Payel Sen
• 金额: $ 26.82万
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10913080
• 关键词: Aging; Alzheimer' s disease brain; Area; Brain; Cognitive deficits; Collaborations; Data; Expression Profiling; Fiber; Flow Cytometry; Gene Expression Profiling; Genes; Goals; Immunofluorescence Immunologic; Impaired cognition; Link; Magnetic Resonance Imaging; Microglia; Molecular; Mus; Neutrophil Infiltration; Proteins; Resolution; age related; aging brain; differential expression; transcriptomics; white matter

In the past year, we have continued our analysis of spatial transcriptomics data from aging murine brains. We found evidence of microglia activation and neutrophil infiltration in the aging brain which we validated using flow cytometry and immunofluorescence. Interestingly, these changes occurred prominently in the white matter fiber tracts which led us to hypothesize that this area may show structural damage. We confirmed with MRI that this was indeed the case (in collaboration with Dr. Dan Benjamini).

在过去的一年中，我们继续分析衰老小鼠大脑的空间转录组学数据。我们发现了衰老大脑中小胶质细胞激活和中性粒细胞浸润的证据，并使用流式细胞术和免疫荧光进行了验证。有趣的是，这些变化主要发生在白质纤维束中，这使我们推测该区域可能显示出结构损伤。 我们通过 MRI 证实情况确实如此（与 Dan Benjamini 博士合作）。