Genome-wide profiling of 5-hydroxymethylcytosine in aging liver

衰老肝脏中 5-羟甲基胞嘧啶的全基因组分析

• 批准号: 10467891
• 负责人: Payel Sen
• 金额: $ 11.11万
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10467891
• 关键词: Aging; CRISPR/Cas technology; Characteristics; Coupled; DNA; DNA Modification Process; Epigenetic Process; Gene Expression; Genes; Genetic Transcription; Immunoprecipitation; Laboratories; Link; Liver; Mitochondria; Modification; Molecular; Natural regeneration; Pathologic; Play; Proteomics; Reader; Rejuvenation; Reporting; Tissues; Work; age related; clinically relevant; epigenomics; genome-wide; in vitro Model; liver function; multiple omics; novel; transcriptomics

In our original proposal we reported the global increase in 5 hydroxymethylcytosine (5hmC) in aging livers. In the past year, we have probed the hydroxymetholome landscape of the aging liver in greater detail. Using hydroxymethylcytosine DNA immunoprecipitation coupled to sequencing (hmeDIP-seq), we found that 5hmC accumulates in the bodies of genes involved in mitochondrial function. 5hmC also serves to restrict changes in gene expression during aging contributing to transcriptional stability.

在我们最初的提案中，我们报告了衰老肝脏中 5 羟甲基胞嘧啶 (5hmC) 的整体增加。 在过去的一年中，我们更详细地探讨了衰老肝脏的羟基酶活性。使用羟甲基胞嘧啶 DNA 免疫沉淀结合测序 (hmeDIP-seq)，我们发现 5hmC 在参与线粒体功能的基因体内积累。 5hmC 还可以限制衰老过程中基因表达的变化，从而有助于转录稳定性。