AMINOPEPTIDASES IN NORMAL AND CATARACT LENSES

正常晶状体和白内障晶状体中的氨基肽酶

基本信息

批准号：
2459141
负责人：
KRISHNA K SHARMA
金额：
$ 11.69万
依托单位：
UNIVERSITY OF MISSOURI-COLUMBIA
依托单位国家：
美国
项目类别：
财政年份：
1993
资助国家：
美国
起止时间：
1993-08-01 至 1998-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2459141
关键词：
aminopeptidase animal tissue cataract chemical binding crystallins electrofocusing high performance liquid chromatography hydrolysis laboratory mouse laboratory rabbit laboratory rat lens lens proteins molecular pathology oligopeptides peptidases personal computers proteolysis

项目摘要

The relatively long half life of lens proteins has been attributed to the low level and tight control of proteolytic activity. During the last few years the accumulation of peptides in senile cataract and every cataract model has been documented. It has been hypothesized that the limited proteolysis of the crystallins by lens proteases and the incomplete removal of the peptides generated may contribute to cataract development. However, in spite of extensive studies on lens proteolytic system, breakdown of the peptides generated by proteolysis has not been investigated. Our previous studies have shown that the aminopeptidase III we identified and isolated from lens may be the physiologically important major peptidase in lens. We have now isolated an acylpeptide hydrolase from bovine lenses which can remove N-acetyl amino acids from the peptides. Our studies have also shown that the lens contains aminopeptidases capable of complete hydrolysis of peptides resistant to leucine aminopeptidase indicating a major role for other enzymes in lens. We have proposed to continue our investigations in search of new aminopeptidases in lens. We will also be studying the hydrolysis of specific peptide sequences by lens peptidases, prolyl oligopeptidase and acylpeptide hydrolase. The proposal also includes the study of peptide interaction with crystallins and the effect of peptide accumulation "in vivo".

晶状体蛋白的一半寿命相对较长。低水平和严格控制蛋白水解活性。期间最近几年，肽在老年白内障中的积累并且已经记录了每个白内障模型。它一直假设晶状体对结晶蛋白的有限蛋白水解蛋白酶和生成的肽的不完全去除可能有助于白内障发育。但是，尽管有广泛的晶状体蛋白水解系统的研究，肽的分解尚未研究由蛋白水解产生的。我们以前的研究表明，氨基肽酶III我们从镜头鉴定和隔离可能是生理上重要的镜头中的主要肽酶。现在我们已经分离了酰基肽水解酶从牛透镜中可以去除N-乙酰基氨基酸肽。我们的研究还表明，镜头包含能够完全耐肽的氨基肽酶亮氨酸氨基肽酶指示其他酶在镜片。我们建议继续我们的调查以寻找新的镜片中的氨基肽酶。我们还将研究特异性肽序列通过晶状体肽酶，prolyl寡肽酶和酰基肽水解酶。该提议还包括研究肽与结晶蛋白的相互作用和肽的作用积累“体内”。

项目成果

期刊论文数量（15）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Purification and characterization of prolyl oligopeptidase from bovine lens.

牛晶状体脯氨酰寡肽酶的纯化和表征。

DOI：
10.1006/exer.1994.1086
发表时间：
1994
期刊：
Experimental eye research
影响因子：
3.4
作者：
Sharma,KK;Ortwerth,BJ
通讯作者：
Ortwerth,BJ

Association between hearing and vision impairments in older adults.

DOI：
10.1111/j.1475-1313.2011.00876.x
发表时间：
2012-01
期刊：
Ophthalmic & physiological optics : the journal of the British College of Ophthalmic Opticians (Optometrists)
影响因子：
0
作者：
Schneck ME;Lott LA;Haegerstrom-Portnoy G;Brabyn JA
通讯作者：
Brabyn JA

Identification of new lens protease(s) using peptide substrates having in vivo cleavage sites.

使用具有体内切割位点的肽底物鉴定新的晶状体蛋白酶。