Metastable Crystallins: Structure and Stabilization

亚稳态晶体蛋白：结构和稳定性

基本信息

批准号：
9769023
负责人：
KRISHNA K SHARMA
金额：
$ 38.75万
依托单位：
UNIVERSITY OF MISSOURI-COLUMBIA
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-05-01 至 2022-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9769023
关键词：
Adult Amyloid beta-Protein Arginine Back Biological Biological Response Modifier Therapy Cataract Cell Culture System Cell Culture Techniques Cells Chemicals Complex Crosslinker Crystalline Lens Crystallins Development Disease Etoposide Event Excision Future Goals Hela Cells High temperature of physical object Human Hydrogen Peroxide Hydrophobicity In Vitro Injury Investigation Knowledge Maintenance Modeling Modification Molecular Molecular Chaperones Onset of illness Oxidative Stress Preparation Property Protein Conformation Proteins Recombinants Role S-crystallin Site Site-Directed Mutagenesis Staurosporine Stress Structure Structure-Activity Relationship Testing Therapeutic Urea alpha-Crystallins base biophysical techniques fascinate improved in vitro activity insight lens lens transparency macromolecule mutant novel preservation prevent protein oligomer replication stress response small molecule stem thermal stress tool

项目摘要

ABSTRACT -Crystallin is a complex macromolecule that accounts for nearly 40% of the adult lens proteins. The chaperone-like activity of -crystallin, is implicated as a key component in the maintenance of lens transparency by suppression of crystallin aggregation. In vitro studies of α-crystallin have shown that chaperone activity is increased when α-crystallin is subjected to heat (48-60oC) and then brought back to 25- 37oC. Similarly, α-crystallin subjected to urea-induced unfolding and refolding also displays increased chaperone activity. Understanding the molecular organization and properties of crystallin subunits in activated chaperones would help answer questions on how -crystallin chaperone-like activity might be harnassed and manipulated for the development of protein-based therapeutics. In our studies of the role of subunit interactions in chaperone activity, a recombinant αB-crystallin expressed after deleting the 54-61 sequence (resulting in a protein designated as αB∆54-61) was found to form ~ 40 % smaller oligomer than the wild-type protein oligomer but to show a 10-fold increase in chaperone activity. The Specific Aims of this project will uncover the molecular changes that account for the increased chaperone activity in heat- and urea-treated α-crystallin and deletion mutant of αB-crystallin. Specific Aim 1: a) Determine the mechanism of αB-crystallin chaperone activation after deletion of the 54-61 sequence and b) determine the biological implications of enhanced chaperone activity in cell culture system. Specific Aim 2: a) Determine the functional units and mechanism of activation in α-crystallin chaperone after thermal stress and urea-induced unfolding and refolding and b) investigate the cytoprotective effect of heat- and urea-activated crystallins. Novel cross-linker(s) will be used to gain fresh insights into the “cryptic” chaperone sites getting exposed in the activated crystallins. The studies will also make use of site-directed mutagenesis, mass spectrometric analysis and biophysical techniques to uncover the molecular changes at the secondary and tertiary structure levels and to delineate the quaternary organization of the subunits in the oligomers showing increased chaperone activity. To see whether the activated α-crystallins can be exploited to protect cells from oxidative injury, the effects of stress-inducing agents such as H2O2, staurosporine or etoposide will be investigated in Cos-7, HeLa, HEK293 and ARPE-19 cells. Further, the ability of activated chaperones to suppress aggregation of mutant proteins (αAG98R) as well as fibril-forming β-amyloid will be investigated both in vitro and ex-vivo. The long-term goals of the studies are to understand the structure–function relationship of activated α-crystallin and develop crystallin proteins that have therapeutic value in protein conformational diseases.

抽象的 α-晶状体蛋白是一种复杂的大分子，占成人晶状体蛋白的近 40%。 α-晶状体蛋白的分子伴侣样活性，是维持晶状体的关键成分通过抑制晶状体蛋白聚集来提高透明度。 α-晶状体蛋白的体外研究表明，当 α-晶状体蛋白受热 (48-60oC) 然后恢复到 25- 类似地，α-晶状体蛋白在尿素诱导下解折叠和重折叠也表现出增加。了解激活的晶状体蛋白亚基的分子组织和特性。伴侣将有助于回答有关如何利用α-晶状体蛋白伴侣样活动的问题，以及在我们对亚基相互作用作用的研究中进行操纵以开发基于蛋白质的疗法。在分子伴侣活性中，删除 54-61 序列后表达的重组 αB-晶状体蛋白（导致发现指定为 αBΔ54-61 的蛋白质形成的寡聚体比野生型蛋白质小约 40% 寡聚物，但显示伴侣活性增加 10 倍。该项目的具体目标将揭示分子变化导致经过热处理和尿素处理的 α-晶状体蛋白中分子伴侣活性增加， αB-晶状体蛋白的缺失突变体。具体目标 1：a) 确定 αB-晶状体蛋白伴侣的机制。删除 54-61 序列后激活，b) 确定增强的生物学意义细胞培养系统中的分子伴侣活性。具体目标 2： a) 确定分子伴侣的功能单位和机制。热应激和尿素诱导的去折叠和重折叠后α-晶状体蛋白伴侣的激活和b) 研究热和尿素激活的晶状体蛋白的细胞保护作用。获得对活性晶状体蛋白中暴露的“神秘”伴侣位点的新见解。还将利用定点诱变、质谱分析和生物物理技术来揭示二级和三级结构水平的分子变化并描绘四级结构寡聚物中亚基的组织显示伴侣活性是否增加。活化的 α-晶状体蛋白可用于保护细胞免受氧化损伤、应激诱导的影响 H2O2、十字孢菌素或依托泊苷等药物将在 Cos-7、HeLa、HEK293 和 ARPE-19 中进行研究此外，激活的伴侣还具有抑制突变蛋白（αAG98R）聚集的能力。因为纤维形成的β-淀粉样蛋白将在体外和离体进行研究。研究的长期目标是。了解激活的 α-晶状体蛋白的结构-功能关系并开发晶状体蛋白对蛋白质构象疾病具有治疗价值。