Longitudinal and noninvasive characterization of brain function and HIV expression in a novel HIV transgenic rat

新型 HIV 转基因大鼠脑功能和 HIV 表达的纵向和非侵入性表征

• 批准号: 10932028
• 负责人: Michael Michaelides
• 金额: $ 56万
• 依托单位: NATIONAL INSTITUTE ON DRUG ABUSE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10932028
• 关键词: Brain; Cocaine; Dopamine; Drug Exposure; Exposure to; Gene Expression; Generations; Goals; HIV; Heroin; Human; Knowledge; Mediating; Metabolic Marker; Monitor; Neuroimmune; Positron-Emission Tomography; Proteins; Rat Transgene; Rattus; Reporter; Research; Rodent; Therapeutic Intervention; comorbidity; drug of abuse; experimental study; molecular imaging; neuroinflammation; neurotransmission; novel; radioligand; response; therapeutic development

The goal will be to monitor, in rats, changes across several different brain parameters associated with HIV exposure using translational molecular imaging with positron emission tomography (PET). We also aim to use a novel PET reporter to examine factors that can alter HIV gene expression in the rodent brain. This will occur using either AAV-mediated approaches as well as generation of a novel HIV/ChRERa+ transgenic rat. Rats will be exposed to different drugs of abuse (e.g., cocaine, heroin) and regional HIV expression in the brain will be monitored in a non-invasive and longitudinal manner.

目的是在大鼠中，使用带正电子发射断层扫描（PET）的转化分子成像相关的几种与HIV暴露相关的不同大脑参数的变化。我们还旨在使用新颖的宠物报道者来检查可以改变啮齿动物大脑中HIV基因表达的因素。这将使用AAV介导的方法以及新型HIV/Chrera+转基因大鼠的产生。将以非侵入性和纵向的方式监测大鼠的大鼠滥用药物（例如可卡因，海洛因）和大脑中的区域HIV表达。