Studies of Thyroid Function in Health and Disease

健康和疾病中的甲状腺功能研究

• 批准号: 10931304
• 负责人: Joanna Klubo-Gwiezdzinska
• 金额: $ 9.17万
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10931304
• 关键词: 2019-nCoV; Acute; Adrenal Glands; Affect; Age; Aldosterone; Androstenedione; Biological; Blood; Board Certification; Body Composition; Clinical; Corticosterone; Corticotropin; Cortisone; Cortodoxone; Data; Diagnosis; Diagnostic Procedure; Differentiation and Growth; Discipline of Nuclear Medicine; Disease; Endocrine; Endocrinology; Energy Metabolism; Enrollment; Extracorporeal Membrane Oxygenation; Family history of; Female; Follicle Stimulating Hormone; Functional disorder; Genetic; Health; Hospitalization; Hydrocortisone; Hydroxyprogesterone; Hyperthyroidism; Hypothyroidism; Immunoassay; Laboratory Study; Literature; Luteinization; Magnetic Resonance Imaging; Mass Spectrum Analysis; Measurement; Metabolism; Patients; Physical Examination; Plasma; Progesterone; Protocols documentation; Public Health; Radioactive Iodine; Recording of previous events; SARS-CoV-2 infection; Sampling; Scanning; Severity of illness; Steroids; Symptoms; Technetium; Testing; Testosterone; Thyroid Function Tests; Thyroid Gland; Thyroid Hormones; Thyrotropin; Thyroxine; Training; Training Programs; Triiodothyronine; United States National Institutes of Health; Urine; X-Ray Computed Tomography; biomedical referral center; cell growth; dehydroepiandrosterone; imaging study; liquid chromatography mass spectrometry; male; participant enrollment; patient screening; profiles in patients; research study; severe COVID-19; sex; steroid hormone; uptake

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection is associated with adrenal and thyroid dysfunction. However, the current literature is based on immunoassay measurements, which are subject to several interfering factors. In this preliminary study, we performed mass spectrometry analysis of steroid and thyroid hormone profiles in patients (18 years) hospitalized with SARS-CoV-2 at two tertiary referral centers. Liquid chromatography-mass spectrometry analysis of adrenocortical steroids progesterone, corticosterone, 17-hydroxyprogesterone, 11-deoxycortisol, cortisol, cortisone, dehydroepiandrosterone (DHEA), androstenedione, testosterone, total thyroxine (TT4), total triiodothyronine (TT3), reverse triiodothyronine (rT3), and immunoassay analysis of adrenocorticotropic hormone (ACTH), thyroid stimulating hormone (TSH), luteinizing and follicle-stimulating hormones, and aldosterone were performed. Data were dichotomized based on extracorporeal membrane oxygenation (ECMO) and survival status. Plasma analysis of a total of 63 plasma samples from ten patients (four females; median age: 41.5 years) revealed a median 11-deoxycortisol elevation up to 38-fold in male ECMO patients compared to non-ECMO patients, and up to 20-fold among female ECMO patients who died from SARS-CoV-2 compared to non-ECMO surviving patients. The 11-deoxycortisol elevations were more profound and out of proportion to corresponding cortisol levels. Significantly higher levels were noted in plasma DHEA in male non-survivors, androstenedione in females with severe SARS-CoV-2, corticosterone, and aldosterone in male/female non-survivors. Median TT4, TT3, and rT3 levels were significantly lower in patients on ECMO and among non-survivors. In conclusion, the adrenal and thyroid axes can be affected during acute SARS-CoV-2 and the degree of disruption may be associated with disease severity, sex, and ECMO status.

严重的急性呼吸综合征冠状病毒-2（SARS-COV-2）感染与肾上腺和甲状腺功能障碍有关。但是，目前的文献基于免疫测量测量，这些测量受到几个干扰因素的影响。在这项初步研究中，我们对在两个三级推荐中心住院的患者（18岁）对类固醇和甲状腺激素谱进行了质谱分析。脂质色皮质类固醇孕激素，皮质酮，17-羟基种蛋白，11-脱氧皮质醇，可皮质醇，可质子酮，脱氢性运动剂（DHEA），甲状腺素（DHEA），抗睾丸激素，睾丸激素，TRINE（TTTTRII），TRII（tttrii）（TTRII），进行三碘甲醇（RT3）和免疫测定分析，分析肾上腺皮质激素（ACTH），甲状腺刺激激素（TSH），黄体和刺激性激素和脂肪刺激激素和醛固酮。基于体外膜氧合（ECMO）和生存状况将数据二分化。血浆分析来自10例患者（四名女性；中位年龄：41.5岁）的63个血浆样品显示，与非ECMO患者相比，男性ECMO患者的中位数为11-脱氧甲状质醇的升高，高达38倍，与SARS-COV-2患者相比，女性ECMO患者的中位数高达38倍。 11-脱氧皮质醇的升高更加深刻，并且与相应的皮质醇水平不成比例。在男性非活体中血浆DHEA中的水平明显更高，在男性/女性非幸存者中患有严重的SARS-COV-2，皮质酮和醛固酮的女性中，雄性雌雄同体的水平明显更高。 ECMO和非幸存者中的患者中值TT4，TT3和RT3水平明显降低。总之，在急性SARS-COV-2期间可能会影响肾上腺和甲状腺轴，并且破坏程度可能与疾病的严重程度，性别和ECMO状态有关。

项目成果

Combined Immunosuppressive Therapy Induces Remission in Patients With Severe Type B Insulin Resistance: A Prospective Cohort Study.

• DOI: 10.2337/dc18-0884
• 发表时间: 2018-11
• 期刊: Diabetes care
• 影响因子: 16.2
• 作者: Klubo-Gwiezdzinska J;Lange M;Cochran E;Semple RK;Gewert C;Brown RJ;Gorden P
• 通讯作者: Gorden P

Catecholamine physiology and its implications in patients with COVID-19.

• DOI: 10.1016/s2213-8587(20)30342-9
• 发表时间: 2020-12
• 期刊: The lancet. Diabetes & endocrinology
• 作者: Gubbi S;Nazari MA;Taieb D;Klubo-Gwiezdzinska J;Pacak K
• 通讯作者: Pacak K

Combined Immunosuppressive Therapy for Severe Graves Dermopathy.

• DOI: 10.7326/l21-0152
• 发表时间: 2021-10
• 期刊: Annals of internal medicine
• 影响因子: 39.2
• 作者: Gubbi S;Araque KA;Avadhanula S;Azam A;Merkel R;Cochran C;Burbelo PD;Waldman M;Neumann S;Gershengorn MC;Skarulis M;Klubo-Gwiezdzinska J
• 通讯作者: Klubo-Gwiezdzinska J

The -258A/G (SNP rs12885300) polymorphism of the human type 2 deiodinase gene is associated with a shift in the pattern of secretion of thyroid hormones following a TRH-induced acute rise in TSH.

人类 2 型脱碘酶基因的 -258A/G (SNP rs12885300) 多态性与 TRH 诱导的 TSH 急剧升高后甲状腺激素分泌模式的转变有关。

• DOI: 10.1530/eje-11-1073
• 发表时间: 2012
• 期刊: European journal of endocrinology
• 影响因子: 5.8
• 作者: Peltsverger,MayaY;Butler,PeterW;Alberobello,AnnaTeresa;Smith,Sheila;Guevara,Yanina;Dubaz,OrnellaM;Luzon,JavierA;Linderman,Joyce;Celi,FrancescoS
• 通讯作者: Celi,FrancescoS

Prevalence of Hypothyroidism in Patients With Erdheim-Chester Disease.

• DOI: 10.1001/jamanetworkopen.2020.19169
• 发表时间: 2020-10-01
• 期刊: JAMA network open
• 影响因子: 13.8
• 作者: Shekhar S;Sinaii N;Irizarry-Caro JA;Gahl WA;Estrada-Veras JI;Dave R;Papadakis GZ;Tirosh A;Abel BS;Klubo-Gwiezdzinska J;Skarulis MC;Gochuico BR;O'Brien K;Hannah-Shmouni F
• 通讯作者: Hannah-Shmouni F