UF PASS Administrative Supplement: Regulation of exercise transducers

UF PASS 行政补充：运动传感器的监管

基本信息

批准号：
10746522
负责人：
Karyn A Esser
金额：
$ 34.91万
依托单位：
UNIVERSITY OF FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-12-15 至 2023-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10746522
关键词：
Acute Administrative Supplement Biological Specimen Banks Biomedical Research Biotin Blood COVID-19 pandemic Cell Nucleus Colon Companions Coupled Data Data Analyses Data Set Development Disease Enzymes Epigenetic Process Exercise Funding Gene Expression Genetic Transcription Genomics Goals Health Human In Vitro Kidney Label Liver Lung Manuscripts Maps Methodology Methods Modeling Molecular Muscle Nuclear Paper Pathway interactions Peripheral Phase Physical activity Plasmids Postdoctoral Fellow Prevention Proteins Proteome Proteomics Publications RNA Processing Rattus Regulation Resources Sampling Site Skeletal Muscle Streptavidin Testing Tissues Transducers Writing animal facility chromatin remodeling design exercise training experience experimental study in vivo insight mRNA Expression novel pandemic disease release factor response tool transcription factor transcriptome sequencing transcriptomics

项目摘要

Summary Given the importance of physical activity in healthy living and prevention and treatment of many diseases, it is not surprising that determining the molecular mechanisms or “map” of the exercise response has become an important focus of biomedical research. The Molecular Transducers of Physical Activity in Humans Consortium (MoTrPAC) is a multicenter consortium that is currently being designed to achieve this important goal. The UF PASS Phase 2 experiments have been focused on two goals. The first has been to develop the use of a new method to support the discovery of factors released from muscle during exercise. The tool is the TurboID enzyme that can label proteins in close proximity with a biotin moiety. With this labeling we can selectively extract proteins derived from skeletal muscle with streptavidin beads from other peripheral tissues and blood. The second goal of our Phase 2 experiments is focused on direct determination of transcription factors within the nucleus following acute exercise to support the known changes in gene expression. To approach this challenge, we have optimized isolation of nuclei from tissue, starting with muscle tissue. We have identified enriched clusters of proteins that are associated with transcription, RNA processing and chromatin remodeling that provide insight in the muscle changes. For this supplement, we would like to take these studies to publication with inclusion of additional tissues such as liver, kidney and colon. We have proposed the following three specific aims with associated manuscripts: Specific Aim 1. To develop the TurboID methodology for discover of proteins released from muscle at 4 and 24hrs following an acute bout of exercise. Manuscript 1 Specific Aim 2. To define the nuclear proteome changes with acute exercise in liver, lung and muscle at immediate post exercise and 1hr post exercise. Identify novel pathways discovered with this approach, map targeted transcription factor changes coupled with gene expression with deep RNA sequencing. Manuscript 2 Specific Aim 3. To write, at least one other manuscript using MoTrPAC PASS data already available. One plan is to focus on the acute transcriptional response to exercise data in muscle, and other tissues to define common and unique genomic and transcriptomic responses.

概括鉴于身体活动对于健康生活以及预防和治疗许多疾病的重要性，毫不奇怪，确定运动反应的分子机制或“图”已经身体活动的分子传感器成为生物医学研究的一个重要焦点。人类联盟 (MoTrPAC) 是一个多中心联盟，目前旨在实现这个重要的目标。 UF PASS 第二阶段实验的重点是两个目标，第一个目标是开发使用一种新方法来支持发现运动过程中肌肉释放的因子。 TurboID 酶可以标记与生物素部分非常接近的蛋白质。可以用链霉亲和素珠从其他外周选择性地提取源自骨骼肌的蛋白质我们第二阶段实验的第二个目标是直接测定组织和血液。剧烈运动后细胞核内的转录因子支持基因的已知变化为了应对这一挑战，我们从组织中优化了细胞核的分离，首先是我们已经鉴定出与转录相关的丰富蛋白质簇， RNA 处理和染色质重塑可提供对肌肉变化的洞察。作为补充，我们希望将这些研究发表，其中包括其他组织，例如肝脏、肾脏和结肠。我们通过相关手稿提出了以下三个具体目标：具体目标 1. 开发 TurboID 方法来发现 4 岁时肌肉释放的蛋白质以及剧烈运动后 24 小时手稿 1。具体目标 2. 确定肝、肺和肌肉急性运动时核蛋白质组的变化确定运动后立即和运动后 1 小时发现的新途径，通过深度 RNA 测序绘制目标转录因子变化与基因表达的结合。手稿2 具体目标 3. 为了撰写，至少一篇使用 MoTrPAC PASS 数据的其他手稿已可用。一项计划是关注肌肉和其他组织对运动数据的急性转录反应，定义常见和独特的基因组和转录组反应。

项目成果

期刊论文数量（17）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Targeted brain-specific tauopathy compromises peripheral skeletal muscle integrity and function.

靶向大脑特异性 tau 蛋白病会损害周围骨骼肌的完整性和功能。

DOI：
10.1101/2023.11.17.567586
发表时间：
2023
期刊：
bioRxiv : the preprint server for biology
影响因子：
0
作者：
Alava,Bryan;Hery,Gabriela;Sidhom,Silvana;Prokop,Stefan;Esser,Karyn;Abisambra,Jose
通讯作者：
Abisambra,Jose

Optimization of the Omni-ATAC protocol to chromatin accessibility profiling in snap-frozen rat adipose and muscle tissues.

DOI：
10.1016/j.mex.2022.101681
发表时间：
2022
期刊：
METHODSX
影响因子：
1.9
作者：
Nair, Venugopalan D.;Vasoya, Mital;Nair, Vishnu;Smith, Gregory R.;Pincas, Hanna;Ge, Yongchao;Douglas, Collin M.;Esser, Karyn A.;Sealfon, Stuart C.
通讯作者：
Sealfon, Stuart C.

Time of Day and Muscle Strength: A Circadian Output?