ECHO Laboratory Core at Vanderbilt for Integrated Sample Biobanking and Processing

范德堡大学 ECHO 实验室核心，用于集成样本生物库和处理

• 批准号: 10745188
• 负责人: Suman Ranjan Das
• 金额: $ 730万
• 依托单位: VANDERBILT UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2030-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10745188
• 关键词: Academic Medical Centers; Address; Architecture; Area; Basic Science; Behavioral; Bioinformatics; Biological; Biological Assay; Biological Markers; Biological Response Modifiers; Blood; Cells; Chemicals; Child Development; Child Health; Childhood; Clinical; Clinical Data; Cloud Computing; Collaborations; Collection; Complex; Cotinine; DNA; Data; Data Analyses; Data Analytics; Development; Diet; Dimensions; Disease; Ensure; Environmental Exposure; Environmental Risk Factor; Epidemiology; Epigenetic Process; Exposure to; Feces; Funding; Genetic; Genomics; Goals; Hair; Health; Human Milk; Infection; Infrastructure; Investments; Isoprostanes; Laboratories; Lead; Leadership; Life; Life Style; Link; Measures; Metadata; Metagenomics; Methods; Mission; Molecular; Nail plate; Nicotine; Nose; Nuclear; Nucleic Acids; Outcome; Participant; Pathogenesis; Patients; Performance; Perinatal; Placenta; Positioning Attribute; Proteomics; Protocols documentation; Public Health; RNA; Research; Research Design; Research Personnel; Resource Sharing; Resources; Risk Factors; Running; Sample Size; Sampling; Science; Secure; Site; System; Tissues; Translational Research; United States National Institutes of Health; Universities; Urine; Validation; analysis pipeline; assay development; biobank; clinical research site; clinical translation; cohort; data analysis pipeline; data exchange; data integration; data management; data sharing; early childhood; early life exposure; experience; high throughput screening; insight; member; metabolomics; microbiome; microbiome research; multiple omics; novel; programs; quality assurance; response; sample collection; social; success; trait; transcriptome sequencing; transcriptomics

SUMMARY Early-life environmental exposures (e.g., social-environmental, parental risk factors, nicotine, diet, infection) are increasingly implicated in the early pathogenesis of childhood diseases that have life-long consequences. Mechanisms linking these exposures to longer-term outcomes remain limited. In 2016, the NIH established the Environmental influences on Child Health Outcomes (ECHO) Program, a collaborative multi-dimensional research initiative to characterize the impact of early-life environmental factors on childhood health (>70 cohorts, >50K participants). While targeted assays within ECHO are likely to lead to disease-specific insight, broad, comprehensive, unbiased assessment of the molecular space for novel discovery—a key mission of ECHO— will necessitate centralization of biobanking efforts/laboratory management with capability for high-throughput “OMICs”/non-“OMICs” assay as well as novel assay development, bioinformatics, and cloud architecture/data sharing for collaborative science. In response to RFA-OD-22-016, Vanderbilt will address this need by establishing the ECHO Laboratory Core at Vanderbilt for Integrated Sample Biobanking and Processing (ELVIS). ELVIS facilitates the collection and processing of biospecimens; manages the biorepository; performs a wide range of biospecimen assays (including novel development), and coordinates metadata and assay data transfer to the Coordinating Center. ELVIS is organized in core “resources” to provide leadership/integration to manage ECHO biobanking, assay performance, and data delivery: (1) administrative/LIMS/biobanking; (2) metabolomics; (3) proteomics; (4) nucleic acid assessment; (5) metagenomics; (6) bioinformatics/study design. We are uniquely positioned for this initiative, leveraging Vanderbilt’s unique long-term strategic investment in functional biobanking and assay: (1) large-scale, reliable biorepository receipt and laboratory management (“LIMS”) capability (>350K patients currently with biospecimens; many other NIH funded biobanks); (2) nationally recognized systems for clinical metadata capture (REDCap, REDBrics; used in NIH initiatives, like All of Us); (3) cutting-edge laboratory cores with capability for novel assay development/validation. We will establish harmonized protocols and workflow for ECHO cohort biospecimen collection and tracking infrastructure from the point of sample collection to long-term storage (Aim 1); perform high-quality, well-powered multi-OMICs and targeted assays to identify molecular correlates of disease trajectories in early life (Aim 2), and provide comprehensive data management platform to facilitate integrated data analysis (Aim 3). ELVIS is an ideal mechanism for ECHO given (1) deep, funded experience in handling the requisite sample sizes in banking and high-throughput assay, including quality assurance measures; (2) prior track record in ECHO to ensure ECHO- specific metadata collection, curation, and harmonization; (3) secure methods for cloud infrastructure for data analysis pipelines and data flow to clinical sites and data analysis center. Successful completion will enable the success of ECHO’s mission to discover molecular underpinnings of early childhood determinants of disease.

概括 早期生活环境暴露（例如，社会环境，父母风险因素，尼古丁，饮食，感染） 越来越多地涉及到具有终身后果的儿童疾病的早期发病机理。 将这些暴露与长期结局联系起来的机制仍然有限。 2016年，NIH建立了 环境影响儿童健康成果（ECHO）计划，这是一个合作的多维 研究旨在表征早期生命环境因素对儿童健康的影响的主动性（> 70个队列， > 50K参与者）。虽然Echo中的有针对性的阿萨斯可能会导致疾病特异性的见解，但很广泛， 对新发现的分子空间的全面，无偏见的评估，这是Echo的关键使命 - 将有必要对生物群的集中化/实验室管理，并具有高通量的能力 “ OMICS”/非“ OMICS”测定以及新颖的测定开发，生物信息学和云体系结构/数据 共享协作科学。为了响应RFA-OD-22-016，范德比尔特将解决这一需求 在范德比尔特建立回声实验室核心，用于集成样品生物库和处理 （猫王）。猫王促进了生物测量的收集和加工；管理生物座；执行 广泛的生物测量评估（包括新开发）和坐标元数据和测定数据 转移到协调中心。埃尔维斯（Elvis 管理回声生物库，测定性能和数据传递：（1）行政/LIMS/BIOBANKing； （2） 代谢组学； （3）蛋白质组学； （4）核酸评估； （5）宏基因组学； （6）生物信息学/研究设计。 我们为这项倡议提供了独特的位置，利用范德比尔特在 功能性生物群和测定：（1）大规模，可靠的生物座席收据和实验室管理 （“ lim”）功能（>当前患有生物测量的患者> 35万名；许多其他NIH资助的生物库）； （2）全国 公认的临床元数据捕获系统（Redcap，Redbrics；与我们所有人一样，用于NIH计划中）； （3） 尖端实验室核心具有新的测定开发/验证能力。我们将建立 Echo队列的生物镜收集和跟踪基础架构的协调协议和工作流程 收集样品的长期存储点（AIM 1）；执行高质量的，有力的多词和 有针对性的测定方法以鉴定早期生命中疾病轨迹的分子相关性（AIM 2），并提供 综合数据管理平台，以促进集成数据分析（AIM 3）。猫王是理想的 ECHO的机制给定（1）在处理银行和银行中必要样本规模的深度，资助的经验 高通量测定法，包括质量保证措施； （2）ECHO中的事先记录以确保Echo- 特定的元数据收集，策展和协调； （3）用于数据的云基础架构的安全方法 分析管道和数据流到临床站点和数据分析中心。成功完成将使 Echo的使命是发现疾病早期决定剂的分子基础的使命。