Washington University Chronic KidneyDisease National Resource Center

华盛顿大学慢性肾病国家资源中心

• 批准号: 10747719
• 负责人: BENJAMIN D. HUMPHREYS
• 金额: $ 90.48万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10747719
• 关键词: Acceleration; Address; Adopted; Affect; Area; Bar Codes; Benign; Biological Assay; CRISPR/Cas technology; Cardiovascular system; Cell Culture Techniques; Cell Nucleus; Cells; Chronic; Chronic Kidney Failure; Code; Communication; Communities; Consultations; Data Analyses; Development; Disease; Diverse Workforce; Doctor of Medicine; Doctor of Philosophy; Education; Equipment; Evolution; Family; Fibrosis; Functional disorder; Funding; Genes; Genetic; Genetic Techniques; Genomic medicine; Genus Hippocampus; Goals; In Vitro; Incubators; Injury; Injury to Kidney; Kidney; Lead; Leadership; Metabolic; Metabolism; Modeling; Morbidity - disease rate; Mus; Pathogenicity; Pathologic; Patients; Population; Pre-Clinical Model; Proteins; Protocols documentation; RNA Splicing; Radioactive; Research; Research Personnel; Resolution; Resource Development; Resource Sharing; Resources; Scientific Advances and Accomplishments; Services; Students; Techniques; Technology; Testing; Time; Tissues; Tracer; Training; Tubular formation; Underrepresented Minority; United States National Institutes of Health; Universities; Validation; Variant; Washington; Work; bioinformatics pipeline; biomedical resource; computational pipelines; cost; genetic testing; genetic variant; hands on instruction; improved; in silico; in situ sequencing; in vitro Assay; in vivo; kidney biopsy; kidney metabolism; metabolomics; mortality; multiple omics; novel therapeutic intervention; oxidation; programs; recruit; response to injury; single cell technology; stable isotope; summer student; technological innovation; tool; transcriptomics; variant of unknown significance

Project Summary/Abstract: Overall Component The Washington University Chronic Kidney Disease National Resource Center is focused on the significant problem of chronic kidney disease (CKD), which affects almost 15% of the US population and carries significant morbidity and mortality. Several scientific advances have the promise to accelerate CKD research, but many of these advances are not accessible to the kidney research community due to limited expertise and/or the need for expensive equipment. Thus, this NRC will address the fundamental challenge of providing better access to cutting-edge techniques in single cell omics, genetics, and metabolism to the kidney research community to facilitate advances in CKD research. Dr. Ben Humphreys is the overall program director, and this Center will consist of four Cores: an Administrative Core, two Biomedical Resource Cores, and a Resource Development Core. The Administrative Core will oversee distribution of funds across the Cores, communicate with the National O’Brien Consortium, manage the Summer Student Enrichment Program, and promote the involvement of early stage investigators and a diverse workforce. The Variant Validation Core, one of the Biomedical Resource Cores, investigates the pathogenicity of genetic variants of uncertain significance using CRISPR/Cas9-gene editing, in silico approaches, and tailored in vitro assays. The Metabolism Core, the other Biomedical Resource Core, provides consultation for users and access to a number of metabolic assays to interrogate changes in kidney metabolism relevant to CKD. These assays include Seahorse bioflux analysis, radioactive substrate oxidation assays of tissue ex vivo, untargeted metabolomics, and stable isotope tracer studies. The Metabolism Core will also work with the O’Brien Consortium to provide validated protocols for assays commonly performed (e.g. Seahorse assays on primary cells) and provide hands’ on training using these protocols. The Single Cell Omics Research Evolution (SCORE) Core is the Resource Development Core, which develops protocols and bioinformatics pipelines for cutting-edge techniques like split pool barcoding for single nuclei multi-omics and high resolution in-situ sequencing-based spatially resolved transcriptomics. All four Cores will work together and with the network of O’Brien National Resource Centers to make scientific advancements more accessible to the kidney research community with particular emphasis on junior investigators and development of a diverse biomedical workforce.

项目摘要/摘要：整体组件 华盛顿大学慢性肾脏疾病国家资源中心的重点 慢性肾脏疾病（CKD）的问题，影响了几乎15％的美国人口，并且携带 显着的发病率和死亡率。几个科学进步有望加速CKD研究， 但是，由于专业知识有限，肾脏研究社区无法获得许多进步 和/或需要昂贵的设备。这是NRC将解决提供的基本挑战 更好地获取单细胞轨迹，遗传学和代谢的尖端技术 社区促进CKD研究的进步。 Ben Humphreys博士是总体计划主任，这 中心将由四个核心组成：一个行政核心，两个生物医学资源核心和一个资源 发展核心。行政核心将监督核心跨核心的资金分配，交流 通过国家奥布莱恩联盟，管理夏季学生丰富计划，并促进 早期研究人员和潜水员劳动力的参与。变体验证核心，其中之一 生物医学资源核心研究了使用不确定意义的遗传变异的致病性 CRISPR/CAS9基因编辑，用于计算机方法和量身定制的体外测定法。新陈代谢核心，另一个 生物医学资源核心，为用户提供咨询，并访问许多代谢测定法 质疑与CKD相关的肾脏代谢的变化。这些测定包括Seahorse BioFlux分析， 组织离体，无靶向代谢组学和稳定的同位素示踪剂的放射性底物氧化测定法 研究。代谢核心还将与O'Brien联盟合作，为 通常执行的测定法（例如，在主细胞上进行海马测定），并使用 这些协议。单细胞OMICS研究演化（得分）核心是资源开发 核心，为诸如拆分池之类的尖端技术开发协议和生物信息学管道 单核多词的条形码和基于空间分辨的高分辨率基于原位测序 转录组学。这四个核心将共同合作，并与奥布莱恩国家资源中心的网络一起工作 使肾脏研究社区更容易获得科学进步，特别强调 初级研究人员和潜水员生物医学劳动力的发展。