Proteomics

蛋白质组学

• 批准号: 10626984
• 负责人: Allis Soe-Lang Chien
• 金额: $ 6.09万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-04 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10626984
• 关键词: Address; Antibodies; Antibody-drug conjugates; Antineoplastic Agents; Aptitude; Area; Biological; Biological Assay; Budgets; Cancer Biology; Cancer Center Support Grant; Chemicals; Clinical; Communication; Communities; Complement; Complex; Computer software; Consultations; Crystallization; Custom; Data; Data Analyses; Data Files; Development; Doctor of Philosophy; Drug Kinetics; Education; Experimental Designs; Fostering; Funding; Future; Genomics; Goals; Health Services Research; Institution; Investments; Knowledge; Laboratories; Language; Licensing; Ligation; Malignant Neoplasms; Mass Spectrum Analysis; Metabolism; Methodology; Methods; Modernization; Molecular Target; Outcome; Pathway interactions; Persons; Post Translational Modification Analysis; Preparation; Proteins; Proteomics; Proteomics Shared Resource; Protocols documentation; Publications; Reproducibility; Research; Research Personnel; Research Support; Resource Sharing; Resources; Sampling; Scientist; Series; Services; Signal Transduction; Software Tools; System; Techniques; Technology; Therapeutic; Training; Update; Walking; Water; anticancer research; biomarker discovery; cancer stem cell; clinically relevant; cohort; crosslink; data access; data archive; data exchange; design; drug discovery; empowerment; experience; improved; instrument; instrumentation; mass spectrometer; member; method development; programs; sharing platform; small molecule; transcriptomics; user-friendly

PROJECT SUMMARY The Proteomics Shared Resource (P-SR) is focused on fostering high-impact cancer-relevant research by providing SCI members with access to state-of-the-art, user-friendly mass spectrometry-based resources and expertise. The P-SR operates 15 mass spectrometer systems that serve ~60 SCI researchers annually. Research support goals are accomplished via the following technical capabilities (1) full-service research partnership from experiment design through sample preparation and mass spectrometric analysis to data analysis; (2) broad-spectrum proteomic support from pilot discovery through complex quantitative assays; (3) customizable quantitative small molecule and clinical scale proteomic assays, taking advantage of modern advances in multiplexing and targeted techniques; (4) intact protein characterization, including antibodies and antibody-drug conjugates; (5) open access walk-up instrumentation and hands-on experience; and (6) online and in-person education, training, and network-building opportunities. These technical capabilities are foundational to numerous scientific areas, such as (1) discovering relevant molecular targets in cancer; (2) providing accurate, orthogonal means of validating research findings from genomic and transcriptomic analyses at the protein level; (3) supporting cancer drug discovery efforts with pharmacokinetics and metabolism analytics; and (4) investigating biological pathways and signaling mechanisms relevant to cancer, both for target and biomarker discovery. Since the last review, the P-SR has continued to acquire new high-end instrumentation, including a Thermo Scientific Q Exactive HF-X and a Waters Xevo TQ-XS. Members of all programs use the P-SR, with the heaviest use coming from the Cancer Biology and Cancer Stem Cells and Cancer Therapeutics programs. In 2020, 41% of users were SCI investigators. The P-SR contributed to 10 cancer-focused publications (5 high-impact). The annual budget of the P-SR is $1,547,560, yet the CCSG request is $70,495. Accordingly, the P-SR leverages extensive institutional support and seeks only 5% from CCSG funds.

项目摘要 蛋白质组学共享资源（P-SR）着重于通过 为SCI成员提供访问最先进的，用户友好的基于质谱的资源和 专业知识。 P-SR运行15个质谱仪系统，每年为60个SCI研究人员提供服务。 研究支持目标是通过以下技术能力实现的（1）全方位服务研究 从实验设计到样品制备和质谱分析到数据的合作伙伴关系 分析; （2）通过复杂的定量测定试验发现的广谱蛋白质组学支持； （3） 利用现代化的优势 多路复用和目标技术的进步； （4）完整的蛋白质表征，包括抗体和 抗体 - 药物缀合物； （5）开放访问步行仪器和动手经验； （6）在线 以及面对面的教育，培训和网络建设机会。这些技术能力是 众多科学领域的基础，例如（1）发现癌症中相关的分子靶标； （2） 提供从基因组和转录组分析验证研究结果的准确的正交手段 在蛋白质水平； （3）支持药代动力学和代谢分析的癌症药物发现工作； （4）研究与癌症相关的生物学途径和信号传导机制 生物标志物发现。 自上次评论以来，P-SR继续获取新的高端仪器，包括Thermo 科学的Q精确HF-X和A Waters Xevo TQ-XS。所有程序的成员使用P-SR，最重 使用癌症生物学和癌症干细胞和癌症治疗剂计划的使用。在2020年，41％ 用户是SCI调查人员。 P-SR促成了10个以癌症为中心的出版物（5个高影响力）。这 P-SR的年度预算为1,547,560美元，但CCSG请求为70,495美元。因此，P-SR的利用 广泛的机构支持，仅从CCSG基金中寻求5％。