Proteomics

蛋白质组学

• 批准号: 8375610
• 负责人: Allis Soe-Lang Chien
• 金额: $ 4.01万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8375610
• 关键词: Advisory Committees; Archives; Cancer Center; Communities; Core Facility; Data; Data Analyses; Development; Education; Faculty; Fees; Funding; Future; Goals; Housing; Individual; Ions; Laboratories; Location; Mass Spectrum Analysis; Methods; Mission; Peer Review; Proteomics; Proteomics Shared Resource; Protocols documentation; Publications; Recovery; Research; Research Personnel; Resource Sharing; Resources; Robot; Sampling; Scientist; Services; System; Technology; Universities; anticancer research; base; computerized data processing; cost; design; empowered; experience; instrumentation; mass spectrometer; meetings; member; method development; nano; research study; scaffold; square foot; user-friendly

The Proteomics Shared Resource offers state-of-the-art, user-friendly mass spectrometry-based resources to support Cancer Center members. In addition to making available facilities and services, the Core enables education, methods development and new applications, all designed to meet the rapidly evolving needs of researchers in cancer research. The Core operates under the direction of Dr. Allis Chien, with oversight by an interdisciplinary faculty advisory committee. It occupies 2200 sq ft of space in a conveniently accessible central campus location, and employs four staff scientists in addition to Dr. Chien. Currently, the core houses six mass spectrometers and associated instrumentation; these include single quad, ion trap, triple quad, and Q-Tof mass spectrometers, as well as 2-D nano-LC systems and nanospray robots. In-house proteomics data processing capabilities include Sequest, Mascot, Scaffold, and MaxQuant. Annual operating costs are approximately $400,000; as an A21-compliant cost center, the facility operates on a cost-recovery basis, with ongoing expenses recovered primarily through user fees. In 2008, the labs of 32 Cancer Center members comprised 52% of the facility's user base, up from 40% in 2007. Future goals include the implementation of new MS instrumentation, expansion of quantitative proteomics services, and enhancement of data processing capabilities.

蛋白质组学共享资源提供了最先进的，用户友好的质谱资源，以支持癌症中心成员。除了提供可用的设施和服务外，核心还可以使教育，方法开发和新应用程序旨在满足癌症研究中研究人员的快速发展需求。 核心在Allis Chien博士的指导下运作，并由跨学科的教师咨询委员会监督。它在便利的中央校园位置占据了2200平方英尺的空间，除了Chien博士外，还雇用了四名员工科学家。目前，核心容纳六个质谱仪和相关的仪器；其中包括单四核，离子陷阱，三尺度和Q-TOF质谱仪，以及二维纳米LC系统和纳米喷雾机器人。内部蛋白质组学数据处理功能包括续集，吉祥物，脚手架和最大。 年度运营成本约为40万美元；作为一个符合A21的成本中心，该设施以成本率基础运行，主要通过用户费用收回了持续的费用。 2008年，32个癌症中心成员的实验室占该设施用户群的52％，高于2007年的40％。未来的目标包括实施新的MS仪器，扩展定量蛋白质组学服务以及增强数据处理能力。