Conformational Energetics and Heterogeneity to Reveal Gating Mechanisms of TRPV and TRPM Ion Channels

构象能量学和异质性揭示 TRPV 和 TRPM 离子通道的门控机制

• 批准号: 10627103
• 负责人: Sharona E Gordon
• 金额: $ 6.65万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-01 至 2027-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10627103
• 关键词: Address; Adenosine Diphosphate Ribose; Administrative Supplement; Amber; Biochemistry; Biophysics; Codon Nucleotides; Faculty; Fluorescence; Fluorescence Resonance Energy Transfer; Fluorometry; Funding; Grant; Heterogeneity; Immunoprecipitation; Ion Channel; Ions; Laboratories; Learning; Measurement; Mentors; Mentorship; Methodology; Molecular; Molecular Conformation; Parents; Research; Research Assistant; Research Project Grants; Scientific Inquiry; Time; Training; Transition Elements; Work; career; career development; career networking; member; patch clamp; professor; programs; success; sulfated glycoprotein 2; tool; unnatural amino acids

Project Summary/Abstract This request for an administrative supplement to facilitate career re-entry will allow Dr. Jill B. Jensen to pursue a path to independence as a Research Assistant Professor under the mentorship of Dr. Sharona Gordon. She will work on the TRPM2 project in the scope of the parent R35 grant, engage in career development, and develop an independent research program. This project addresses the molecular mechanisms governing Ca2+- and ADP Ribose-driven conformational rearrangements in TRPM2 channels. Dr. Jensen will train under Dr. Gordon to gain expertise in transition metal ion FRET using both steady-state and time-resolved fluorescence measurement; using amber codon suppression to introduce unnatural amino acids; and patch clamp fluorometry. She will learn quantitative multiplex immunoprecipitation in the laboratory of Dr. Stephen Smith. Work on this project will provide Dr. Jensen with tools in biochemistry and molecular biophysics to expand her scientific reach. With active mentoring support from Dr. Gordon, Dr. Wordeman, and Dr. Ramirez and directed career development activities, Dr. Jensen will also develop an independent line of scientific inquiry, expand her professional network, increase national visibility, train as a mentor, and apply for her own research grant, as described in the research plan.

项目摘要/摘要 该要求促进职业重新进入的行政补充要求，将允许Jill B. Jensen博士追求 在Sharona Gordon博士的指导下，作为研究助理教授的独立途径。她 将在父母R35赠款范围内从事TRPM2项目，从事职业发展并发展 一个独立的研究计划。该项目解决了有关CA2+ - 和ADP的分子机制 核糖驱动的构象重排在TRPM2通道中。詹森博士将在戈登博士的领导下训练 使用稳态和时间分辨荧光获得过渡金属离子货物的专业知识 测量;使用琥珀密码子抑制引入非天然氨基酸；和贴片夹的荧光测定法。 她将在斯蒂芬·史密斯（Stephen Smith）博士的实验室中学习定量多重免疫沉淀。解决这个问题 项目将为Jensen博士提供生物化学和分子生物物理学的工具，以扩大其科学影响力。 在戈登博士，Wordeman博士和Ramirez博士的积极指导下，并执导了职业 开发活动，詹森博士还将开发独立的科学询问线，扩大她 专业网络，提高国家知名度，训练作为导师，并申请自己的研究赠款，作为 在研究计划中描述。