Conformational Energetics and Heterogeneity to Reveal Gating Mechanisms of TRPV and TRPM Ion Channels

构象能量学和异质性揭示 TRPV 和 TRPM 离子通道的门控机制

• 批准号: 10605108
• 负责人: Sharona E Gordon
• 金额: $ 6.62万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-01 至 2027-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10605108
• 关键词: Address; Adenosine Diphosphate Ribose; Administrative Supplement; Amber; Codon Nucleotides; Fluorescence; Fluorescence Resonance Energy Transfer; Fluorometry; Funding; Grant; Heterogeneity; Ion Channel; Ions; Measurement; Mentors; Methods; Molecular; Molecular Conformation; Parents; Research; Time; Training; Transition Elements; Work; career; career development; patch clamp; programs; success; sulfated glycoprotein 2; unnatural amino acids

Project Summary/Abstract This request for an administrative supplement to promote diversity will allow Dr. Teresa M Swanson to train as a postdoctoral scholar in the Gordon lab. She will work on the TRPM2 project in the scope of the parent R35 grant. This project addresses the molecular mechanisms governing Ca2+- and ADP Ribose-driven conformational rearrangements in TRPM2 channels. Dr. Swanson will train directly under Dr. Gordon in the experimental methods required: transition metal ion FRET using both steady-state and time-resolved fluorescence measurements; using amber codon suppression to introduce unnatural amino acids; and patch-clamp fluorometry. Work on this project will provide Dr. Swanson with excellent training to advance her career. She will also participate in career development opportunities and programs to enhance diversity of the biomedical workforce, as described in the training plan.

项目概要/摘要 为了促进多样性而提出的行政补充请求将使 Teresa M Swanson 博士能够接受培训 戈登实验室的博士后学者。她将在母公司 R35 拨款范围内从事 TRPM2 项目的工作。 该项目研究了控制 Ca2+- 和 ADP 核糖驱动构象的分子机制 TRPM2 通道中的重新排列。斯旺森博士将在实验中直接在戈登博士的指导下接受培训 所需方法：使用稳态荧光和时间分辨荧光的过渡金属离子 FRET 测量；使用琥珀密码子抑制引入非天然氨基酸；和膜片钳 荧光测定法。该项目的工作将为斯旺森博士提供良好的培训，以促进她的职业生涯。她会 还参与职业发展机会和计划，以增强生物医学的多样性 劳动力，如培训计划中所述。