Core A: Ultrastructural Assessment & Human Tissue

核心 A：超微结构评估

• 批准号: 10627575
• 负责人: Olujimi A Ajijola
• 金额: $ 33.97万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-10 至 2028-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10627575
• 关键词: 3-Dimensional; Achievement; Adrenergic Agents; Biological; Cardiac; Cardiomyopathies; Chemicals; Cicatrix; Data Set; Ensure; Family suidae; Functional disorder; Goals; Heart; Heart Diseases; Heart Transplantation; Human; Human Resources; Image; Infrastructure; Ion Channel; Lasers; Link; Lung; Maps; Methods; Molecular; Molecular Biology; Myocardial; Organ Procurements; Organ Transplantation; Patients; Penetration; Process; Productivity; Protocols documentation; Pulmonary Heart Disease; Recovery; Research Personnel; Resolution; Services; Structure; Structure of stellate ganglion; Sudden Death; Techniques; Testing; Therapeutic; Time; Tissue Procurements; Tissues; Validation; cell type; cost; experience; high resolution imaging; human disease; human tissue; innovation; instrumentation; member; mortality risk; neural; neuroregulation; neurotransmission; success; transcriptomics

SUMMARY/ABSTRACT – Scientific Core - Structure The goal of the Ultrastructural Assessment and Human Tissue Core is to maximize the scientific progress and productivity of the PPG investigators by providing essential services. The primary function of Core A is to serve human and porcine myocardial and stellate ganglion tissue needs across all projects and Core B. Given significant regulatory and logistical hurdles needed to procure and study human heart and stellate ganglion tissues, it is ideal for this to be handled by a central core. The core will perform three key functions for the study: 1) tissue clearing and ultrastructural imaging; 2) procurement of normal (or near normal) and diseased human tissues, specifically hearts and stellate ganglia; and 3) molecular biology. All 3 projects of this PPG with utilize at least two of these services, and there will be tight links between this core and Core B, the Functional Assessment and Instrumentation Core. Over the past 15 months, we have built a pipeline to obtain diseased hearts from patients undergoing orthotopic heart transplantation, and normal or near normal hearts rejected for organ transplant. Our infrastructure for obtained stellate ganglia from patients with cardiomyopathy undergoing stellectomy, and stellate ganglia from patients without cardiopulmonary disease at the time of heart and/or lung donation has existed for 5 years. Given the unpredictable times at which these tissues become available, centralizing the tissue procurement activities within this core will benefit the PPG by streamlining protocols and approvals, minimizing cost, and boosting productivity. The Core will also streamline the 4-week process of tissue clearing from pigs and humans by batching tissues to minimize error, tissue clearing involves numerous steps and chemicals. Finally, it is most ideal that tissues are cleared and imaged in high resolution in close coordination with study team members from Projects 1-3 in this Core whether or not the tissues are cleared. For the duration of the PPG, the core is poised – with experience, robust protocols, and experienced personnel – to provide critical services that ensure the success of this PPG.

摘要/摘要 - 科学核心 - 结构 超微结构评估和人体组织核心的目的是最大化科学进步和 PPG调查人员的生产力通过提供基本服务。核心A的主要功能是服务 人类和猪心肌和星状神经节组织在所有项目中都需要以及核心B。 采购和研究人类心脏和星状神经节所需的重大监管和后勤障碍 组织，这是由中心核心处理的理想选择。核心将对 研究：1）组织清除和超微结构成像； 2）采购正常（或接近正常）并解散 人体组织，特别是心脏和星状神经节； 3）分子生物学。该PPG的所有三个项目 使用至少两种服务，并且该核心和核心B之间将存在紧密的联系 评估和仪器核心。在过去的15个月中，我们建立了一条管道以获取散布 来自接受原位心脏移植的患者的心脏，拒绝正常或接近正常心脏的心脏 器官移植。我们从患有心肌病患者的星状神经节获得的基础设施 心脏和/或肺部患者的明星切除术和没有心肺疾病的患者的星状神经节 捐赠已经存在了5年。考虑到这些时间可用的不可预测时间， 将组织采购活动集中在此核心中，将通过简化协议和 批准，最小化成本并提高生产力。核心还将简化4周的过程 通过批处理时间从猪和人类中清除组织以最大程度地减少误差，组织清除涉及许多 步骤和化学物质。最后，最理想的是，将组织清除和成像高分辨率 无论时间是否清除，与该核心项目1-3的研究团队成员的协调。 在PPG期间，核心被中毒 - 具有经验，健壮的协议和经验丰富的人员 - 提供关键服务以确保该PPG的成功。