Mechanisms of Cardiac TRPV1 Afferent Remodeling in Ventricular Arrhythmias

室性心律失常中心脏 TRPV1 传入重塑的机制

基本信息

批准号：
10674847
负责人：
Olujimi A Ajijola
金额：
$ 56.61万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-15 至 2025-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10674847
关键词：
Acute Agonist Animals Anti-Arrhythmia Agents Attenuated Axon Biological Availability Calcium Channel Cardiac Cause of Death Chronic Cicatrix Clinical Clinical Management Data Detection Development Drug usage Electrophysiology (science)Epicardium Family suidae Feedback Fiber Functional disorder Goals Heart Inflammation Intervention Ischemia Link Maps Modeling Myocardial Myocardial Infarction Myocardial dysfunction Myocardium Nature Nerve Nerve Fibers Neurons Norepinephrine Oral Pathologic Patient Care Patients Pattern Pharmaceutical Preparations Phase Pilot Projects Play Research Personnel Resiniferatoxin Risk Role Shapes Signal Transduction Site Spinal Spinal Cord Structure Structure of stellate ganglion Sympathetic Nervous System TRPV1 gene Testing Time Translating Ventricular Arrhythmia Ventricular Tachycardia Vertebral column afferent nerve beta-adrenergic receptor clinical translation comparative efficacy excitotoxicity first-in-human glial activation in vivo injured local drug delivery multidisciplinary neural neurochemistry neuroinflammation neuropeptide Y neuroregulation neurotransmission neurotransmitter release novel novel therapeutics prevent release factor side effect sudden cardiac death tool

项目摘要

PROJECT SUMMARY/ABSTRACT Myocardial infarction (MI) predisposes patients to ventricular tachycardia/fibrillation (VT/VF) and sudden cardiac death. After MI, alterations within the cardiac sympathetic nervous system (SNS) have been tightly linked to VT/VF. These alterations include inflammation, structural and functional remodeling within the stellate ganglion, and heterogeneous remodeling of intramyocardial sympathetic nerves in the scar-border zone. These result in enhanced and dysfunctional cardiac sympathetic neurotransmission that lead to VT/VF. Although spinal afferent signaling is enhanced after MI, the arrhythmogenic potential of spinal afferents (via maladaptive interactions with cardiac sympathetic nerves) has not been explored. Based on novel data from our group, the goal of this proposal is to test the hypothesis that chronic enhanced cardiac afferent signaling is the primary driver of sympathetic neural remodeling and dysfunction that causes VT/VF. Pilot studies from our group using epicardial resiniferatoxin (RTX) to deplete cardiac TRPV1 afferents in porcine support the rationale that persistent afferent signaling (beyond the acute ischemic phase) plays a central role in shaping the neural and cardiac substrates that lead to VAs. We will test our hypotheses using novel tools from a multidisciplinary team of investigators in 3 aims, in porcine with MI. In aim 1, we will determine whether post-MI structural, neuroinflammatory, and functional neuronal remodeling within stellate ganglia are caused by persistent TRPV1 afferent signaling. In aim 2, we will determine whether persistent cardiac TRPV1 activation amplifies intramyocardial neurotransmitter release to increase VT/VF risk. This will be accomplished using simultaneous cardiac electrophysiologic mapping and real time in vivo detection of intramyocardial Norepinephrine and neuropeptide Y levels. We will determine whether TRPV1 afferent depletion attenuates arrhythmogenicity by normalizing neurotransmitter release patterns. In aim 3, we will define the optimal site of RTX delivery for clinical management of VT/VF [Epicardial vs. Stellate Ganglion vs. Epidural application]. This will guide clinical translation of afferent neuromodulation. The results of this proposal may shift how arrhythmogenesis is approached after MI, and guide the development of new therapies that prevent altered afferent signaling after MI to fill a major clinical gap.

项目概要/摘要心肌梗死 (MI) 使患者容易出现室性心动过速/颤动 (VT/VF) 和突发性心律失常心源性死亡。 MI 后，心脏交感神经系统 (SNS) 内的变化受到密切关注。与 VT/VF 相关。这些改变包括星状细胞内的炎症、结构和功能重塑神经节，以及疤痕边界区心肌内交感神经的异质重塑。这些会导致心脏交感神经传递增强和功能失调，从而导致 VT/VF。尽管心肌梗死后脊髓传入信号增强，但脊髓传入的致心律失常潜力（通过与心脏交感神经的适应不良相互作用）尚未被探索。基于来自的新颖数据我们小组，该提案的目标是检验以下假设：慢性增强的心脏传入信号传导是交感神经重塑和功能障碍导致 VT/VF 的主要驱动因素。我们小组的初步研究使用心外膜树脂毒素 (RTX) 来消耗心脏 TRPV1 传入神经猪支持这样的基本原理：持续传入信号（超出急性缺血期）发挥着重要作用。在塑造导致 VA 的神经和心脏基质方面发挥着核心作用。我们将使用以下方法来检验我们的假设由多学科研究人员组成的团队针对患有心肌梗死的猪，针对 3 个目标开发了新工具。在目标 1 中，我们将确定 MI 后星状结构、神经炎症和功能神经元重塑是否发生神经节是由持续的 TRPV1 传入信号引起的。在目标 2 中，我们将确定是否持续心脏 TRPV1 激活会放大心肌内神经递质的释放，从而增加 VT/VF 风险。这将使用同步心脏电生理图测和实时体内检测来完成心肌内去甲肾上腺素和神经肽 Y 水平。我们将确定 TRPV1 传入耗竭通过使神经递质释放模式正常化来减弱致心律失常。在目标 3 中，我们将确定 VT/VF 临床管理的 RTX 递送的最佳部位 [心外膜、星状神经节与心室颤动] 硬膜外应用]。这将指导传入神经调节的临床转化。本提案的结果可能会改变心肌梗死后心律失常发生的方式，并指导新疗法的开发防止心肌梗死后传入信号的改变，以填补主要的临床空白。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Metrics of high cofluctuation and entropy to describe control of cardiac function in the stellate ganglion.

DOI：
10.7554/elife.78520
发表时间：
2022-11-25
期刊：
ELIFE
影响因子：
7.7
作者：
Gurel, Nil Z.;Sudarshan, Koustubh B.;Hadaya, Joseph;Karavos, Alex;Temma, Taro;Hori, Yuichi;Armour, J. Andrew;Kember, Guy;Ajijola, Olujimi A.;Zaidi, Mone
通讯作者：
Zaidi, Mone

Afferents Nerves in Atrial Fibrillation: Going Beyond Fight or Flight.

心房颤动中的传入神经：超越战斗或逃跑。

DOI：
10.1016/j.jacep.2022.01.013
发表时间：
2022
期刊：
JACC. Clinical electrophysiology
影响因子：
0
作者：
Hanna,Peter;Ajijola,OlujimiA
通讯作者：
Ajijola,OlujimiA

The promise of cardiac neuromodulation: can computational modelling bridge the gap?

DOI：
10.1113/jp285309
发表时间：
2023-08-03
期刊：
JOURNAL OF PHYSIOLOGY-LONDON
影响因子：
5.5
作者：
Liu，Michael B. B.;Ajijola，Olujimi A. A.
通讯作者：
Ajijola，Olujimi A. A.

Vagal Nerve Stimulation Reduces Ventricular Arrhythmias and Mitigates Adverse Neural Cardiac Remodeling Post-Myocardial Infarction.

DOI：
10.1016/j.jacbts.2023.03.025
发表时间：
2023-09
期刊：
JACC-BASIC TO TRANSLATIONAL SCIENCE
影响因子：
9.7
作者：
Hadaya, Joseph;Dajani, Al-Hassan;Cha, Steven;Hanna, Peter;Challita, Ronald;Hoover, Donald B.;Ajijola, Olujimi A.;Shivkumar, Kalyanam;Ardell, Jeffrey L.
通讯作者：
Ardell, Jeffrey L.