Exosomal miRNA in neuron to astroglial communication in the CNS

中枢神经系统神经元与星形胶质细胞通讯中的外泌体 miRNA

基本信息

批准号：
10621422
负责人：
Yongjie Yang
金额：
$ 6.27万
依托单位：
TUFTS UNIVERSITY BOSTON
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-07-01 至 2023-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10621422
关键词：
Address Adult Affect Astrocytes Award Axon Biological Blood Vessels Brain Caliber Cell Communication Cells Cellular biology Central Nervous System Diseases Coculture Techniques Communication Complement 1q Conceptions Cre driver Data Development Endosomes Exanthema Exercise Genetic Genetic Transcription Glioblastoma Glutamate Transporter Image Immune In Situ In Vitro Injections Knowledge Label Learning Ligands Location Malignant Neoplasms Mediating MicroRNAs Modeling Molecular Monitor Morphogenesis Morphology Motor Neurons Mus Nervous System Trauma Neurodegenerative Disorders Neuroglia Neurons Neurotransmitters PHluorin Pathologic Pathway interactions Physiology Presynaptic Terminals Process Publishing Regulation Research Retinal Ganglion Cells Role Sampling Signal Transduction Spinal Surface Synapses Synaptic Transmission Techniques Testing United States National Institutes of Health Vesicle base career development cell type designer receptors exclusively activated by designer drugs exosome experimental study extracellular vesicles fluorescence imaging genome-wide in vivo insight intercellular communication intravitreal injection myelination nervous system disorder neuronal cell body neurotransmitter release notch protein novel polarized cell receptor sciatic nerve synaptic function transcriptome

项目摘要

Summary This diversity supplement aims to provide an opportunity for Rashed Alananzeh who meets the NIH diversity candidate criteria, to carry out a post-Baccalaureate study in my lab. In this project, Rashed will characterize the secretion of neuronal exosomes from either soma or axon terminals and the subsequent internalization of neuronal exosomes into astrocytes. Although intraluminal vesicles (ILVs), precursors for secreted exosomes, are largely localized in the somatodendritic compartment of cultured neurons, it is unknown whether these ILVs are preferentially secreted from neuronal soma or axons in vivo, given that vesicular secretion of neurotransmitters are predominantly released from axon terminals. By employing sciatic nerve injection model to selectively label spinal motor neuron (SMN) exosomes and intravitreal injection to selectively label retina ganglion cells (RGC), Rashed will specifically investigate and compare whether neuronal exosomes are differentially released from either somatodendritic or axon terminal compartments and whether these exosomes are also differentially taken up into surrounding astrocytes. Understanding the predominant location of exosome secretion from such a polarized cell type as neurons will give important insights into the potential function of those exosomes. By carrying out this project, it will give him the opportunity to understand how to see a project through from conception to finish, learn various injection techniques, deal with the troubleshooting and optimization that are always needed in scientific experiments and learn the best ways to analyze images and data. Overall, this diversity supplement award will greatly prepare Rashed for his research career development.

概括该多样性补充旨在为 Rashed Alanzeh 提供与 NIH 会面的机会多样性候选人标准，在我的实验室进行学士学位后研究。在这个项目中，拉希德将表征神经元外泌体从体细胞或轴突末端的分泌以及随后的神经元外泌体内化成星形胶质细胞。尽管腔内囊泡（ILV）是分泌的外泌体，主要位于培养神经元的体细胞树突区室中，目前尚不清楚考虑到囊泡，这些 ILV 是否在体内优先从神经元胞体或轴突分泌神经递质的分泌主要由轴突末梢释放。通过使用坐骨神经注射模型选择性标记脊髓运动神经元（SMN）外泌体和玻璃体内注射选择性标记标记视网膜神经节细胞（RGC），Rashed 将专门研究并比较神经元外泌体是否从体树突或轴突终末室释放的差异，以及这些是否外泌体也被不同程度地吸收到周围的星形胶质细胞中。了解神经元等极化细胞类型外泌体分泌的主要位置将为这些外泌体的潜在功能提供重要的见解。通过实施这个项目，它将给他有机会了解如何看待一个项目从构思到完成的整个过程，学习各种注入技术，处理科学实验中始终需要的故障排除和优化，学习分析图像和数据的最佳方法。总的来说，这个多元化补充奖将为拉希德因其研究生涯的发展而闻名。