Research Supplements to Promote Diversity in Vascular Graft Research

促进血管移植研究多样性的研究补充

• 批准号: 10619171
• 负责人: Edith Tzeng
• 金额: $ 8.71万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-15 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10619171
• 关键词: African American; Animal Model; Applications Grants; Arteries; Autologous; Autologous Transplantation; Blood Vessels; Bypass; Cardiovascular Diseases; Carotid Arteries; Cells; Elements; Fiber; Future; Goals; Gold; Grant; In Situ; Intervention; Minority Participation; Modeling; Morbidity - disease rate; Research; Sheep; Site; Testing; Vascular Graft; clinical translation; design; doctoral student; implantation; minority student; parent grant; pre-clinical; senescence; single-cell RNA sequencing; transcriptome sequencing

Abstract The parent grant of this application is R01HL159427 entitled “Elastic, Degradable Vascular Grafts with Helical Microfibers”. The goal of the parent grant is to design a cell-free degradable graft that the host can transform into an autologous vascular conduit. A major aim of the grant is to investigate host remodeling of long microfibrous grafts in a sheep carotid artery model. We performed five implantations in senescent sheep. We learned that the graft needed to be more robust for clinical translation. The research focus of this supplement application is to eliminate fiber stacking and increase fiber fusion at the junction as means to make the vascular graft more robust. Moreover, the Co-I of the parent grant, Dr. Iwijn De Vlaminck, informed the team of a recent advancement on single cell RNA sequencing in sheep. This will greatly enrich the information we gather from our sheep study. Therefore, the research aspect of this supplement will focus on the following specific aims in the next 3 years: 1. Eliminate fiber stacking and induce fiber fusion at the junctions in the graft. 2. Identify the type and potential origin of the cells populating the grafts in the sheep study using single cell RNA sequencing. The design of electrowritten vascular grafts and the study of host remodeling are key aspects of the parent grant. However, neither the study of fiber stacking and fusion, nor RNA sequencing of the sheep graft is proposed in the parent grant. We recognize these as important elements of the study after the project initiation. The research proposed in this supplemental application was not deleted by the initial review group. Halle Welch, a first year African American Ph.D. student, is very passionate about the vascular graft project. This supplement will increase the participation of minority students in vascular graft research and prepare Halle well for a future in academic research. Thus, this supplement request is consistent with the spirit of a diversity supplement.

抽象的 此应用程序的父母授予是R01HL159427，标题为“具有弹性，可降解的血管移植 螺旋微纤维”。父母赠款的目标是设计一个无细胞的可降解移植物 转变为自体血管导管。该赠款的主要目的是调查主机改建 绵羊颈动脉模型中的长度微纤维移植物。我们在感觉绵羊中进行了五次植入。 我们了解到，移植物需要更加强大才能进行临床翻译。研究重点 补充应用是为了消除纤维堆叠并增加连接处的纤维融合，以使 血管移植更健壮。此外，父母赠款的共同体Iwijn de Vlaminck博士告知了 最近在绵羊中进行单细胞RNA测序进步的团队。这将大大丰富信息 我们从绵羊学习中收集。因此，该补充的研究方面将重点放在以下 未来三年的具体目标： 1。消除纤维堆叠并在移植物的连接处诱导纤维融合。 2。确定使用单个绵羊研究中填充移植物的细胞的类型和潜在起源 细胞RNA测序。 电riten血管移植物的设计和宿主重塑的研究是父母的关键方面 授予。但是，纤维堆叠和融合的研究和绵羊移植的RNA测序都不是 在父母赠款中提议。在项目计划之后，我们认为这些是研究的重要要素。 最初的审查组未删除此补充应用中提出的研究。哈莉 韦尔奇（Welch），第一年非裔美国人博士学生对血管移植项目非常热衷。这 补充剂将增加少数族裔学生参与血管移植研究，并准备好Halle 为了实现学术研究的未来。那就补充要求与多样性的精神一致 补充。