OU Macromolecular X-ray Facility

OU高分子X射线设备

• 批准号: 8465568
• 负责人: Leonard M Thomas
• 金额: $ 8.48万
• 依托单位: UNIVERSITY OF OKLAHOMA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8465568
• 关键词: Centers of Research Excellence; Collection; Core Facility; Crystallization; Crystallography; Data; Development; Laboratories; Oklahoma; Research; Research Personnel; Robotics; Roentgen Rays; Sampling; Screening procedure; Solutions; System; Universities; X ray diffraction analysis; X-Ray Crystallography; X-Ray Diffraction; career; detector; instrumentation; sample collection; structural biology; three dimensional structure

The development ofthe OU-Norman Macromolecular Crystallography Laboratory (MCL) as a COBRE Core facility will allow it to continue to provide expertise in both the crystallization of macromolecular samples and the collection of X-ray diffraction data for research groups at the University of Oklahoma and throughout the State of Oklahoma. This facility will provide the Junior Investigators (Jls), early career Investigators and other research groups involved in structural biology a state-of-the-art facility to advance their structural studies. The MCL can guide the research groups from the initial setup of crystallization plates to the solution and interpretation of the final three-dimensional structures. The MCL currently provides the capability of high throughput crystallization by the utilization of robotics Instrumentation. Utilization of the robotics for crystallization allows for efficient use of sample in determining macromolecular crystallization conditions. Crystals can be screened by MCL's current diffractometer setup but we are proposing an upgrade of our Xray generator to be able to provide the best possible X-ray crystallography facility for our Junior Investigators and other structural biology Investigators In the State. The increase In Intensity will benefit all investigators by providing the capability of screening and collection of X-ray diffraction data on smaller and generally weaker diffracting crystals. In addition to utilizing our current detector on the higher intensity generator we are also proposing to couple it to a CCD detector to provide a modern efficient diffraction system to allow the Jl's and other investigators statewide to conduct their research In structural biology.

OU-诺曼高分子晶体学实验室 (MCL) 作为 COBRE 核心的发展 设施将使其能够继续提供大分子样品结晶和 为俄克拉荷马大学和整个研究小组收集 X 射线衍射数据 俄克拉荷马州。该设施将为初级调查员（Jls）、早期职业调查员和其他 参与结构生物学的研究小组拥有最先进的设施来推进其结构研究。 MCL 可以指导研究小组从结晶板的初始设置到解决方案和 最终三维结构的解释。 MCL目前提供高的能力 通过利用机器人仪器实现吞吐量结晶。利用机器人技术 结晶可以有效地利用样品来确定大分子结晶条件。 晶体可以通过 MCL 当前的衍射仪设置​​进行筛选，但我们建议升级我们的 X 射线 发生器能够为我们的初级研究人员提供最好的 X 射线晶体学设施 和该州的其他结构生物学研究人员。强度的增加将使所有调查人员受益 通过提供筛选和收集较小且普遍的 X 射线衍射数据的能力 衍射晶体较弱。除了在更高强度发生器上使用我们的电流检测器之外，我们 还建议将其与 CCD 探测器耦合，以提供现代高效的衍射系统，从而允许 Jl 和其他研究人员在全州范围内进行结构生物学研究。