Macromolecular Crystallography Lab

高分子晶体学实验室

• 批准号: 10197144
• 负责人: Leonard M Thomas
• 金额: $ 12.97万
• 依托单位: UNIVERSITY OF OKLAHOMA
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10197144
• 关键词: Biochemistry; Centers of Research Excellence; Chemicals; Chemistry; Complex; Consumption; Core Facility; Coupled; Crystallization; Crystallography; Data; Data Collection; Doctor of Philosophy; Drug Design; Education and Outreach; Educational workshop; Equipment; Evaluation; Faculty; Funding; Goals; Grant; Hybrids; Laboratories; Liquid substance; Maintenance; Molecular; Molecular Structure; Nucleic Acids; Oklahoma; Phase; Postdoctoral Fellow; Preparation; Process; Proteins; Reproducibility; Research; Research Personnel; Resources; Robotics; Roentgen Rays; Sampling; Services; Source; Structural Protein; Structure; Structure-Activity Relationship; Students; System; Time; Training; Training and Education; Universities; X ray diffraction analysis; X-Ray Crystallography; base; computing resources; detector; disease mechanisms study; flexibility; graduate student; improved; insight; instrumentation; interest; macromolecule; operation; outreach; programs; sample collection; screening; small molecule; structural biology; summer program; symposium; three dimensional structure; undergraduate research experience

PROJECT SUMMARY (Macromolecular Crystallography Laboratory) The three-dimensional structure determination of macromolecules such as proteins and nucleic acids using X- ray crystallography provides unique insights into the function of the molecule or molecular complex. The OU- Norman Macromolecular Crystallography Laboratory Core (MCL) provides expertise and state-of-the-art instrumentation to support the Oklahoma COBRE in Structure Biology (OCSB) affiliated research groups and other research groups involved in structural biology research, particularly in protein crystallography, across the State of Oklahoma. The MCL accomplishes this by utilizing robotic instrumentation for the screening of crystallization conditions, the utilization of a high flux micro focus X-ray generator and a state-of-the-art hybrid pixel X-ray detector for data collection along with up to date maintenance of computational resources to process and determine the structure of the macromolecules of interest. The MCL director, Dr. Leonard Thomas, is a full time Ph.D. crystallographer. The facility also employs a Graduate Assistant to aid in the screening of crystallization conditions and the preparation of samples for collection of X-ray data. The MCL provides training to students, postdoctoral fellows and faculty who are interested in macromolecular structure determination. During the OCSB Phase I funding the MCL user base has been greatly expanded and the facility will continue to expand its user base by providing expertise, state-of-the-art facilities and training during the OCSB Phase II.

项目概要 （高分子晶体学实验室） 利用 X- 测定蛋白质、核酸等大分子的三维结构 射线晶体学为分子或分子复合物的功能提供了独特的见解。 OU- 诺曼高分子晶体学实验室核心 (MCL) 提供专业知识和最先进的技术 仪器支持俄克拉荷马州 COBRE 结构生物学 (OCSB) 附属研究小组和 其他参与结构生物学研究的研究小组，特别是蛋白质晶体学研究， 俄克拉荷马州。 MCL 通过利用机器人仪器筛选来实现这一目标 结晶条件、高通量微焦点 X 射线发生器和最先进的混合设备的使用 用于数据收集的像素 X 射线探测器以及计算资源的最新维护 处理并确定感兴趣的大分子的结构。 MCL主任Leonard博士 托马斯，是一名全职博士。晶体学家。该设施还聘请了一名研究生助理来协助 筛选结晶条件和制备用于收集 X 射线数据的样品。内侧副韧带 为对大分子结构感兴趣的学生、博士后和教师提供培训 决心。在 OCSB 第一阶段资助期间，MCL 用户群已大大扩大，并且 设施将通过在期间提供专业知识、最先进的设施和培训，继续扩大其用户群 OCSB 第二阶段。