Mouse Breeding and Procedure Core

小鼠育种和程序核心

• 批准号: 10616534
• 负责人: ANN MARIE SCHMIDT
• 金额: $ 38.55万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-05-01 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10616534
• 关键词: Adipose tissue; Age; Aorta; Atherosclerosis; Bone Marrow Transplantation; Breeding; Cardiometabolic Disease; Dual-Energy X-Ray Absorptiometry; Functional disorder; Gender; Genotype; Hepatic Stellate Cell; Hepatocyte; Individual; Kupffer Cells; Liver; Macrophage; Magnetic Resonance Imaging; Mus; Parabiosis; Partner in relationship; Procedures; Quality Control; Randomized; Reproducibility; Scanning; Services; Tail; Testing; Tissue Transplantation; Transplantation Surgery; Weaning; extracellular vesicles; glucose tolerance; imaging study; insulin tolerance; irradiation; member; programs; sex; ultrasound

Summary The Mouse Breeding and Procedure Core (Core C) is essential for the smooth functioning of the Program Project. Core C has two major functions. First, Core C will provide and oversee mouse breeding and genotyping services. Mice will be bred centrally for all three projects of the Program Project in order to maximize quality control and consistency of mouse lines, and to maximize efficiency, particularly for lines shared across the three Projects. Mice will be mated, born, separated, and weaned by the Core members. The Core will direct tails for genotyping to NYU Core for Genotyping Services. Once genotyped, Core C will allocate mice to either further breeding or to experimentation depending on the needs and the genotype/sexes of mice obtained. Core C will distribute all mice for studies to the three Projects by age 6 weeks. As indicated in the individual Projects, Core C, through Core A, implements randomization and strategies that will be essential for rigor and reproducibility and protection against bias. Second, Core C will coordinate and perform mouse procedures. Shared procedures across the Program Project to be performed and overseen by Core C include: aorta transplantation surgery, lethal irradiation and bone marrow transplantation; adipose tissue transplantation; parabiosis; glucose and insulin tolerance tests; imaging studies (liver ultrasound and magnetic resonance imaging and DEXA scanning); isolation of primary cells from the liver, including hepatocytes, Kupffer cells and hepatic stellate cells and isolation of extracellular vesicles. Core C will serve all three projects of the Program Project over all five years.

概括 小鼠育种和程序核心（核心 C）对于该计划的顺利运行至关重要 项目。 Core C 有两个主要功能。首先，Core C 将提供并监督小鼠育种和基因分型 服务。该计划项目的所有三个项目将集中饲养小鼠，以最大限度地提高质量 鼠标线的控制和一致性，并最大限度地提高效率，特别是对于三个共享的线 项目。小鼠将由核心成员进行交配、出生、分离和断奶。核心将直接尾部 基因分型到纽约大学基因分型服务核心。一旦基因分型，Core C 将把小鼠分配给进一步的 根据需要和获得的小鼠的基因型/性别进行育种或实验。核心C将 在 6 周龄前将所有用于研究的小鼠分配到三个项目。如各个项目所示，核心 C 通过核心 A 实施随机化和策略，这对于严谨性和可重复性至关重要 和防止偏见的保护。其次，Core C 将协调并执行鼠标程序。共享程序 由 Core C 执行和监督的整个计划项目包括：主动脉移植手术、 致命辐射和骨髓移植；脂肪组织移植；联体共生；葡萄糖和胰岛素 耐受性测试；影像学研究（肝脏超声、磁共振成像和 DEXA 扫描）； 从肝脏中分离原代细胞，包括肝细胞、库普弗细胞和肝星状细胞并分离 细胞外囊泡。 Core C 将在五年内为该计划项目的所有三个项目提供服务。