Rapid Allergenic Particle Identification (RAPID)

快速过敏性颗粒识别 (RAPID)

基本信息

批准号：
8073325
负责人：
Kenneth L Shepard
金额：
$ 0.81万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-08-15 至 2011-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8073325
关键词：
5 year old Acute Air Allergens Anterior nares Asthma Beds Biological Assay Biosensor Birth Canis familiaris Child Chronic Cohort Studies Collection Complex Concentration measurement Coupled Data Detection Development Devices Dictyoptera Disease Dust Electronics Enzyme-Linked Immunosorbent Assay European Exposure to Felis catus Fluorescence Fluorescence Resonance Energy Transfer Ginger Goals Home environment House Dust Hypersensitivity Immunoassay Individual Life Location Measurement Measures Methods Microfluidics Monitor Mus New York Nose Plastics Play Protein Array Protein Microchips Proteins Public Health Research Design Research Personnel Risk Role Sampling Semiconductors Series Side Site Technology Time Toxin United States National Institutes of Health Vacuum air sampling antigen binding base cost detector environmental agent epidemiology study improved inner city innovation interest metal oxide novel particle pathogen polydimethylsiloxane pressure programs pyroglyphid sample collection sensor technology development

项目摘要

DESCRIPTION (provided by applicant): The proposed effort combines air sampling devices and highly-integrated protein microarrays to permit estimation of multiple protein allergen exposures in a time efficient manner. Portable air sampling devices that fit inside the nostrils and have been used in Australian and European asthma studies will be used for collection of samples in an ongoing study of inner-city New York homes of children (n=200) with increased risk for developing allergy and asthma. Highly multiplexed protein microarrays, allowing parallel assessment of many allergens, integrated onto active complementary metal-oxide-semiconductor (CMOS) substrates will be employed. The use of CMOS allows highly integrated and sensitive devices to be developed because of the immediate proximity of the detector to the sensing electronics. As the commodity technology for microelectronics, such active substrates can be produced at very low cost. An established fluorescence based sensor substrate will be employed for initial studies, augmented by simple microfluidic delivery. Biosensor technology development will include efforts toward real-time measurement capabilities including more complex microfluidic delivery (including pressure activated valves), real-time fluorescence sensing through lifetime-sensitive FRET, and CMOS-integrated mass-based biosensors. The same principles for assessment of allergens in air can be applied to assessment of bacterial and fungal pathogens and their constitutive toxins. These studies have far-reaching implications for acute and chronic measures of public health.

描述（由申请人提供）：拟议的工作将空气采样装置和高度集成的蛋白质微阵列结合起来，以允许以省时的方式估计多种蛋白质过敏原暴露。澳大利亚和欧洲哮喘研究中使用的可安装在鼻孔内的便携式空气采样装置将用于对纽约市中心儿童家庭（n = 200）过敏风险增加的一项正在进行的研究中收集样本和哮喘。将采用高度复合的蛋白质微阵列，允许并行评估许多过敏原，并将其集成到活性互补金属氧化物半导体（CMOS）基板上。由于探测器紧邻传感电子设备，因此使用 CMOS 可以开发高度集成且灵敏的设备。作为微电子学的商品技术，这种有源基板可以以非常低的成本生产。已建立的基于荧光的传感器基板将用于初步研究，并通过简单的微流体输送进行增强。生物传感器技术的开发将包括努力实现实时测量能力，包括更复杂的微流体输送（包括压力激活阀）、通过寿命敏感的 FRET 进行的实时荧光传感，以及 CMOS 集成的基于质量的生物传感器。评估空气中过敏原的相同原理可以应用于评估细菌和真菌病原体及其组成毒素。这些研究对公共卫生的急性和慢性措施具有深远的影响。