Protein Chemistry Core

蛋白质化学核心

基本信息

批准号：
7970030
负责人：
Deborah Morrison
金额：
$ 18.55万
依托单位：
DIVISION OF BASIC SCIENCES - NCI
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7970030
关键词：
Acetylation Amino Acids Buffers Communities Computer software DNA Sequencing Facility Digestion Dimensions Extramural Activities Gel High Pressure Liquid Chromatography Immunoblotting Intramural Research Isoelectric Focusing Label Laboratories Liquid substance Maps Mass Spectrum Analysis Methods Methylation Modification Molecular Weight N-terminal National Institute of Neurological Disorders and Stroke Peptide Mapping Peptides Phase Phosphoamino Acids Phosphorylation Phosphorylation Site Positioning Attribute Post-Translational Protein Processing Process Protein Analysis Protein Chemistry Proteins Proteomics Radioactive Radioactive Sequencing Research Personnel Sampling Serine Services Site Staining method Stains Stroke Techniques Technology Threonine Two-Dimensional Gel Electrophoresis Tyrosine comparative drug modification interest nervous system disorder pressure protein aminoacid sequence two-dimensional

项目摘要

During FY 2008, the Protein Chemistry Core provided services to 12 NCI-CCR investigators. In addition, the Core collaborated with a laboratory in the National Institute of Neurological Disorders and Stroke (NINDS) and an extramural laboratory. - 142 samples were processed for N-terminal peptide sequencing - 79 samples were processed for HPLC peptide analysis - 18 samples were processed for peptide digestion - 53 samples were processed for phosphoamino acid analysis - 73 samples were processed for 2D gel electrophoresis - 69 2D gels were stained or transferred to immobilon for further analysisDuring FY 2008, the Protein Chemistry Core provided services to 12 NCI-CCR investigators. In addition, the Core collaborated with a laboratory in the National Institute of Neurological Disorders and Stroke (NINDS) and an extramural laboratory. - 142 samples were processed for N-terminal peptide sequencing - 79 samples were processed for HPLC peptide analysis - 18 samples were processed for peptide digestion - 53 samples were processed for phosphoamino acid analysis - 73 samples were processed for 2D gel electrophoresis - 69 2D gels were stained or transferred to immobilon for further analysis

2008 财年，蛋白质化学核心为 12 名 NCI-CCR 研究人员提供了服务。此外，该核心还与国家神经疾病和中风研究所 (NINDS) 的一个实验室以及一个校外实验室进行了合作。 - 处理 142 个样品进行 N 端肽测序 - 处理 79 个样品进行 HPLC 肽分析 - 处理 18 个样品进行肽消化 - 处理 53 个样品进行磷酸氨基酸分析 - 处理 73 个样品进行 2D 凝胶电泳 - 69 个 2D 凝胶染色或转移至 Immobilon 进行进一步分析 2008 财年，蛋白质化学核心为 12 名 NCI-CCR 研究人员提供了服务。此外，该核心还与国家神经疾病和中风研究所 (NINDS) 的一个实验室以及一个校外实验室进行了合作。 - 处理 142 个样品进行 N 端肽测序 - 处理 79 个样品进行 HPLC 肽分析 - 处理 18 个样品进行肽消化 - 处理 53 个样品进行磷酸氨基酸分析 - 处理 73 个样品进行 2D 凝胶电泳 - 69 个 2D 凝胶染色或转移至 Immobilon 进行进一步分析