Location-specific In Vivo Sensing and Imaging of Butyrate in the GI Tract

胃肠道中丁酸盐的特定位置体内传感和成像

基本信息

批准号：
10611459
负责人：
Thomas J Mansell
金额：
$ 17.41万
依托单位：
IOWA STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-05-01 至 2025-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10611459
关键词：
Animals Apoptosis Area Bacteria Biological Response Modifier Therapy Biosensor Butyrates Cells Colitis Colon Coupled Crohn&apos s disease Detection Disease Effectiveness of Interventions Engineering Environment Epithelial Cells Escherichia coli Exposure to Fluorescence Gastrointestinal tract structure Genetic Goals Growth Health Human Image Immune response Immunity In Situ In Vitro Inflammation Inflammatory Bowel Diseases Intervention Intestinal Diseases Intestines Knowledge Laboratories Large Intestine Link Location Mammals Maps Measurement Memory Methods Modeling Monitor Mus Operative Surgical Procedures Output Patients Physiology Probiotics Production Protocols documentation Reporter Research Personnel Resolution Small Intestines Supplementation Techniques Technology Testing Volatile Fatty Acids animal imaging design dietary effectiveness evaluation gastrointestinal gastrointestinal epithelium gut bacteria gut microbiome human disease in vivo inflammatory marker luminescence metabolome microbial microbial community microbiome microorganism prebiotics probiotic supplementation rapid technique sensor specific biomarkers translational impact

项目摘要

Project Summary The short chain fatty acid butyrate is a key molecule in the human gut metabolome, regulating bacterial growth, inflammation, and colon cell health. Despite its importance there is currently no reliable method for inline monitoring of butyrate production in situ. In addition, cecal and fecal levels of butyrate are often not good predictors of butyrate production by gut bacteria. The goal of this proposal is to build bacterial biosensors for butyrate which, coupled to cellular memory circuits, will enable monitoring of butyrate content throughout the small and large intestine and biogeographical mapping of butyrate concentrations. This technology will allow researchers to test many different interventions (e.g., prebiotics, probiotics, and other forms of butyrate supplementation) with the goal of optimizing the levels of butyrate in the gut. We will also test interventions that increase butyrate as a proof of concept of the method.

项目摘要短链脂肪酸丁酸酯是人类肠道代谢组的关键分子，调节细菌生长，炎症和结肠细胞健康。尽管它很重要当前没有可靠的方法可以在原位进行丁丽酸酯的生产。此外，丁酸酯的盲肠和粪便水平通常不是丁酸酯的好预测指标肠道细菌的生产。该建议的目的是为与蜂窝记忆电路相连的丁酸酯将有助于监视丁酸酯整个小肠和大肠和生物地理图的内容丁酸酯浓度。这项技术将使研究人员能够测试许多不同的干预措施（例如，益生元，益生菌和其他形式的丁酸酯补充）目的是优化肠道中的丁酸酯水平。我们还将测试将丁理酸作为该方法的概念证明的干预措施。