Location-specific In Vivo Sensing and Imaging of Butyrate in the GI Tract

胃肠道中丁酸盐的特定位置体内传感和成像

基本信息

批准号：
10433447
负责人：
Thomas J Mansell
金额：
$ 21.3万
依托单位：
IOWA STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-05-01 至 2024-02-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10433447
关键词：
Animals Apoptosis Area Bacteria Biological Response Modifier Therapy Biosensor Butyrates Cecum Cells Colitis Colon Coupled Crohn&apos s disease Detection Disease Effectiveness of Interventions Engineering Environment Epithelial Cells Escherichia coli Exposure to Fluorescence Gastrointestinal tract structure Genetic Goals Growth Health Human Image Immune response Immunity In Situ In Vitro Inflammation Inflammatory Bowel Diseases Intervention Intestinal Diseases Intestines Knowledge Laboratories Large Intestine Lead Link Location Mammals Maps Mass Fragmentography Measurement Memory Methods Modeling Monitor Mus Operative Surgical Procedures Output Patients Physiology Probiotics Production Protocols documentation Reporter Research Personnel Resolution Small Intestines Supplementation Techniques Technology Testing Volatile Fatty Acids animal imaging design dietary effectiveness evaluation gastrointestinal gastrointestinal epithelium gut bacteria gut microbiome human disease in vivo inflammatory marker luminescence metabolome microbial microbial community microbiome microorganism prebiotics probiotic supplementation rapid technique sensor specific biomarkers translational impact

项目摘要

Project Summary The short chain fatty acid butyrate is a key molecule in the human gut metabolome, regulating bacterial growth, inflammation, and colon cell health. Despite its importance there is currently no reliable method for inline monitoring of butyrate production in situ. In addition, cecal and fecal levels of butyrate are often not good predictors of butyrate production by gut bacteria. The goal of this proposal is to build bacterial biosensors for butyrate which, coupled to cellular memory circuits, will enable monitoring of butyrate content throughout the small and large intestine and biogeographical mapping of butyrate concentrations. This technology will allow researchers to test many different interventions (e.g., prebiotics, probiotics, and other forms of butyrate supplementation) with the goal of optimizing the levels of butyrate in the gut. We will also test interventions that increase butyrate as a proof of concept of the method.

项目概要短链脂肪酸丁酸酯是人体肠道代谢组中的关键分子，调节细菌生长、炎症和结肠细胞健康。尽管它很重要目前还没有可靠的方法对原位丁酸盐生产进行在线监测。此外，盲肠和粪便中的丁酸盐水平通常不能很好地预测丁酸盐的水平由肠道细菌产生。该提案的目标是构建细菌生物传感器丁酸盐，与细胞记忆电路耦合，将能够监测丁酸盐整个小肠和大肠的含量以及生物地理图丁酸盐浓度。这项技术将允许研究人员测试许多不同的干预措施（例如益生元、益生菌和其他形式的丁酸盐补充剂）目标是优化肠道中的丁酸盐水平。我们也会测试增加丁酸盐的干预措施作为该方法的概念证明。