Multilayer Scaffold for Condyle Surface Regeneration

用于髁表面再生的多层支架

• 批准号: 10604924
• 负责人: Sara Trbojevic
• 金额: $ 4.76万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-15 至 2025-09-14
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10604924
• 关键词: Adult; Architecture; BMP2 gene; Bilateral; Binding; Biochemical; Biocompatible Materials; Biological Assay; Biological Availability; Bone Regeneration; Cartilage; Cells; Chondrocytes; Defect; Diffusion; Fibroblasts; Fibrocartilages; Formulation; Functional Regeneration; Gelatin; Goat; Growth; Growth Factor; Heparin; Heparin Binding; Hyaline Cartilage; Hydrogels; Implant; In Vitro; Infiltration; Injury; Kinetics; Mandible; Mandibular Condyle; Miniature Swine; Modeling; Nanoporous; Natural regeneration; Operative Surgical Procedures; Osteogenesis; Pain; Pathology; Patients; Periodicity; Permeability; Phenotype; Polymers; Porifera; Property; Publishing; Regenerative research; Replacement Arthroplasty; Site; Surface; Technology; Temporomandibular Joint; Temporomandibular Joint Disorders; Time; Tissue Engineering; Tissues; Training; Transforming Growth Factor beta; articular cartilage; bioscaffold; bone; cartilage degradation; cartilage regeneration; cartilaginous; clinically relevant; condylar cartilage; costal cartilage; design; ethylene glycol; in vivo; in vivo regeneration; mechanical load; mechanical properties; migration; novel; osteochondral tissue; osteoprogenitor cell; prevent; regeneration potential; regenerative; regenerative therapy; regenerative treatment; scaffold; skeletal; stem cells; subchondral bone; tissue regeneration; treatment effect

PROJECT SUMMARY The most severe cases of TMJ disorders consist of mandibular condyle degeneration. Unfortunately, no regenerative options exist, and current treatments do not restore full function. The articulating tissue of the condyle is a fibrocartilage that consists of an intricate interface between fibrous, cartilaginous, and boney tissue that is essential for normal function and that is lost in severe TMJ disorders. The objective of this study is to regenerate fibrocartilage-bone interface of the mandibular condyle in skeletally mature goats using a comprehensive tissue engineering approach. I hypothesize that a multilayer scaffold will allow for site-specific fibrous-cartilage-bone regeneration of the mandibular condyle cartilage when compared to a homogenous sponge scaffold and untreated control defects. First, I will study the properties of a multilayer scaffold design in-vitro. I will characterize the permeability and release of TGFβs from the scaffold. Second, I will study the regeneration potential of three cell subpopulations found on the condyle. Third, I will implant our scaffold in mandibular condyle defects of goats and assess their potential for functional regeneration. This proposal highlights the contributions that the PI will make throughout the duration of the training, which will primarily focus on the completion of scaffold fabrication and characterization, as well as the study of the regenerative potential of TMJ cells subpopulations in vitro and of our bilayer scaffold in vivo. Successful completion of this proposal is the critical step to providing a regenerative therapy to treat TMJ mandibular cartilage degeneration and a basis for successful osteochondral tissue regeneration in other sites.

项目摘要 最严重的TMJ疾病病例包括下颌con肤变性。不幸的是，不 存在再生选项，并且当前治疗不会恢复完整的功能。铰接组织 Condyle是一种纤维球，由纤维，软骨和骨头之间的复杂界面组成 对于正常功能至关重要的组织，并且在严重的TMJ疾病中丢失。这项研究的目的 是在骨骼成熟的山羊中再生下颌con的纤维球骨灰碱骨界面 全面的组织工程方法。我假设多层脚手架将允许特定地点 与同质的 赞助商支架和未经处理的控制缺陷。首先，我将研究多层脚手架设计的特性 视野。我将表征TGFβ从支架中释放的渗透性和释放。第二，我将研究 在con上发现的三个细胞亚群的再生潜力。第三，我会植入我们的脚手架 山羊的下颌con虫缺陷，并评估其功能再生的潜力。这个建议 突出了PI在整个培训期间都会做出的贡献，这将 首先关注脚手架制造和表征的完成以及对 TMJ细胞在体外和我们的双层支架中亚群的再生潜力。 成功完成此提案是提供再生疗法治疗TMJ的关键步骤 下颌软骨变性和成功骨软骨组织再生的基础。