Polymer Scaffolds for Mandibular Condyle Cartilage Regeneration

用于下颌髁软骨再生的聚合物支架

基本信息

批准号：
10524746
负责人：
Alejandro Jose Almarza
金额：
$ 66.32万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-12-01 至 2024-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10524746
关键词：
4 year old Adult Architecture Articulation BMP2 gene Bilateral Binding Biochemical Biocompatible Materials Biological Assay Biological Availability Bone Regeneration Cartilage Cells Chondrocytes Chondrogenesis Data Defect Diffusion Family suidae Fibroblasts Fibrocartilages Functional Regeneration Gelatin Goat Growth Heparin Heparin Binding Hyaline Cartilage Hydrogels Implant In Vitro Infiltration Injury Kinetics Liquid substance Mandible Mandibular Condyle Mandibular Scaffold Miniature Swine Modeling Nanoporous Natural regeneration Osteogenesis Pain Pathology Patients Perfusion Periodicity Permeability Phenotype Polymers Population Porifera Property Publishing Regenerative research Replacement Arthroplasty Shapes Site Technology Temporomandibular Joint Temporomandibular Joint Disorders Time Tissue Engineering Tissues Transforming Growth Factor beta articular cartilage base bioscaffold bone cartilage degradation cartilage regeneration cartilaginous clinically relevant condylar cartilage costal cartilage design ethylene glycol fibula healing implant design in vivo in vivo regeneration mechanical load mechanical properties migration novel osteochondral tissue osteoprogenitor cell prevent progenitor regeneration potential regenerative regenerative therapy regenerative treatment scaffold stem cells subchondral bone tissue regeneration treatment effect

项目摘要

The most severe cases of TMJ disorders consist of mandibular condyle degeneration. Unfortunately, no regenerative options exists and current treatments do not restore full function. The articulating tissue of the condyle is a fibrocartilage that consists of an intricate interface between fibrous, cartilaginous, and boney tissue that is essential for normal function and that is lost in severe TMJ disorders. The objective of this study is to regenerate fibrocartilage-bone interface of the mandibular condyle in skeletally mature goats using a comprehensive tissue engineering approach. A condylar defect will be treated with novel multilayer scaffold implant designed to promote site-specific tissue regeneration. We have strong pilot in-vivo data showing that our scaffolds components regenerate fibrous and cartilage tissue in our novel goat model, and bone in a segmental defect model. We will implant the scaffolds in a mediolateral grove-shaped condylar defect. We hypothesize that a multilayer scaffold will allow for site- specific fibrous-cartilage-bone regeneration of the mandibular condyle cartilage when compared to a homogenous sponge scaffold and untreated control defects. First, we will study the properties of a multilayer scaffold design in-vitro. We will characterize the permeability and release of TGFβs from the scaffold. Second, we will assess the functional healing of condylar defects treated with the multilayer scaffolds. We will assess mechanical properties, regenerate tissue composition, and condylar architecture formation using terminal assays at 1, 3 and 6 months post-surgery. Third, we will study the regeneration potential of three cell subpopulations found on the condyle. Successful completion of this proposal is the critical step to provide a regenerative therapy to treat TMJ mandibular cartilage degeneration, and a basis for successful osteochondral tissue regeneration in other sites.

最严重的TMJ疾病病例包括下颌con肤变性。不幸的是，不存在再生选择，当前治疗不会恢复完整的功能。 con的铰接组织是由复杂界面组成的纤维球锥体在纤维，软骨和骨组织之间，这对于正常功能至关重要，即在严重的TMJ疾病中丢失。这项研究的目的是再生纤维球骨骨使用综合组织工程方法。新型多层支架植入物将处理condyar缺陷旨在促进特定地点的组织再生。我们有强大的Vivo Pilot数据表明我们的脚手架成分在我们的新山羊中再生纤维和软骨组织模型和骨骼中的骨骼模型。我们将脚手架植入中外侧树林形的condolar缺陷。我们假设多层支架将允许站点 - 比较时，特异到同质的赞助商支架和未处理的控制缺陷。首先，我们将研究多层脚手架设计的特性。我们将表征渗透性和从支架中释放TGFβ。其次，我们将评估Condylar的功能愈合用多层支架处理的缺陷。我们将评估机械性能，再生使用末端测定1、3和6和6和6 手术后月。第三，我们将研究三个细胞亚群的再生潜力在con上发现。成功完成此提案是提供一个关键步骤再生疗法以治疗TMJ下颌软骨变性，并取得成功的基础其他部位的骨软骨组织再生。