Novel Sample Introduction Methodologies for Molecular Rotational Resonance (MRR) Spectroscopy

分子旋转共振 (MRR) 光谱的新样品引入方法

• 批准号: 10603098
• 负责人: Voislav Blagojevic
• 金额: $ 100.63万
• 依托单位: BRIGHTSPEC, INC.
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-15 至 2025-03-14
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10603098
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BrightSpec Inc. NIH SBIR Application Project Summary Novel Sample Introduction Methodologies for Molecular Rotational Resonance (MRR) Spectroscopy NIH SBIR Direct to Phase II BrightSpec, Inc. Project Summary This project proposes to develop a new analyte sampling technique for Molecular Rotational Resonance (MRR) spectrometers for the volatilization of solid phase analytes. The proposed system, Laser Induced Acoustic Desorption (LIAD), will enable a large number of important compounds to be accessible by MRR, enhancing our understanding in several important areas of research and accelerating pharmaceutical development. The resulting expansion of sampling range and corresponding utility of this valuable technique will greatly accelerate the adoption of MRR in important areas of research and development. MRR is a spectroscopic technique for unambiguous structure determination and quantitation of small molecules, which has been used for decades in academic research laboratories, but only recently commercialized after the recent achievement of several groundbreaking innovations. MRR excels in structural and isomeric analysis, providing a detail of information like nuclear magnetic resonance (NMR), but at a much lower cost and faster speed, with reduced method development. Through working on a final piece of the puzzle, the development of new sampling techniques, BrightSpec seeks to unlock the full analytical potential of this powerful analytical technique to broaden its use throughout a range of applications, including metabolomics, drug discovery, natural product analysis, and the monitoring of environmental pollutants. BrightSpec has evaluated several sampling techniques for volatilization of solid samples and Laser Induced Acoustic Desorption (LIAD) was determined to be uniquely suitable for incorporation with MRR, as the sensitivity of results with these instruments can be adversely affected by the presence of solvents. BrightSpec has evaluated several sampling techniques for volatilization of solid samples and Laser Induced Acoustic Desorption (LIAD was determined to be uniquely suitable for combination with MRR, as the sensitivity of results with these instruments can be adversely affected by the presence of solvents. The project would be conducted by BrightSpec in collaboration with Kenttämaa Labs at Purdue University, which has been developing the LIAD technique for use with mass spectrometry for over 20 years and holds several LIAD-related patents. A proof-of-concept prototype LIAD-MRR instrument has been used to demonstrate the viability of coupling the two techniques. This preliminary work has de-risked this proposed Phase II project, which involves refinement of the prototype to develop a product with the necessary sensitivity, speed, and ease-of-use to facilitate commercial adoption. The resulting product will then be tested thoroughly on relevant samples in market areas where LIAD-MRR is expected to have the greatest impact. Several prospective customer use cases and samples have already been identified. Following this extensive evaluation, the product design will be refined for commercial launch at or before the end of the grant period.

Brightspec Inc. NIH SBIR应用 项目摘要 新型样品简介分子旋转共振方法（MRR）光谱法 NIH SBIR直接进入II期 Brightspec，Inc。 项目摘要 该项目的提案是开发一种用于分子旋转的新分析物采样技术 共振（MRR）光谱仪，用于稳态分析物的挥发。提议 激光诱导的声学解吸（LIAD）系统将使大量重要 MRR可以使用的化合物，增强了我们在几个重要领域的理解 研究和加速药物开发。采样的扩展 这种有价值技术的范围和相应效用将大大加速采用 MRR在研究与开发的重要领域。 MRR是一种光谱技术，用于确定明确的结构和定量 小分子，在学术研究实验室已有数十年的使用，但仅 最近在几项开创性创新成就后，最近商业化了。 MRR在结构和异构分析方面表现出色，提供核等信息的细节 磁共振（NMR），但成本要低得多，速度更快，方法降低 发展。通过制作最终难题，开发新样本 技术，Brightspec试图解锁这种强大的分析的全部分析潜力 在包括代谢组学，药物在内的一系列应用中扩展其使用的技术 发现，自然产品分析以及对环境污染物的监测。 Brightspec评估了几种采样技术，用于稳态样品和激光的挥发 诱导的声学解吸（LIAD）被确定为独特的工作 使用MRR，由于这些仪器的结果的敏感性可能会受到不利影响 存在解决方案。 Brightspec评估了几种抽样技术用于挥发 固体样品和激光诱导的声学解吸（确定LIAD是独特的 适合与MRR组合，因为结果与这些仪器的敏感性可以是 受到解决方案的不利影响。该项目将由Brightspec进行 与普渡大学的Kenttämaa实验室合作，该大学一直在开发LIAD 与质谱一起使用的技术已有20多年，并持有多个与联络有关的技术 专利。 概念验证原型LIAD-MRR仪器已用于证明可行性 耦合这两种技术。这项初步工作使这一提议的II期项目脱离了风险， 涉及原型的细化，以开发具有必要灵敏度的产品， 速度和易用性，以促进商业采用。然后将测试生成的产品 在LIAD-MRR有望拥有最大的市场领域的相关样品中彻底详尽 影响。已经确定了几个潜在的客户用例和样本。 经过这项广泛的评估，产品设计将用于商业发布 或在赠款期结束之前。