Structure and Specificity of Restriction-Modification (R-M) Systems

限制性修饰（R-M）系统的结构和特异性

• 批准号: 10599570
• 负责人: ANEEL K. AGGARWAL
• 金额: $ 9.67万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10599570
• 关键词: Advisory Committees; Archaea; Bacteria; Burkholderia cepacia; Complement; Crystallization; DNA Modification Methylases; DNA Restriction-Modification Enzymes; Doctor of Philosophy; Elements; Fellowship; First Generation College Students; Gene Expression Regulation; Goals; Hispanic; Innate Immune System; Journals; Kinetics; Knowledge; Lead; Methyltransferase; Microbial Biofilms; Modification; Monitor; Motivation; National Institute of General Medical Sciences; Orphan; Paper; Pathogenicity; Publishing; Reading; Research Personnel; Research Training; Specificity; Structure; Talents; Thermodynamics; Training; Training Support; Virulence Factors; Writing; academic preparation; career; cell motility; design; doctoral student; epigenomics; expectation; infancy; low socioeconomic status; meetings; novel strategies; parent grant; underrepresented minority student

Project Summary/Abstract This application is a request for a Diversity Supplement to train and support a talented PhD student, Mr. Richard Quintana-Feliciano, to complement our parent grant (NIGMS R35-GM131780), titled “Structure and specificity of restriction-modification (R-M) systems”. As a Hispanic first-generation student, and coming from a low socioeconomic status, Richard has strived to excel at every step of his academic preparation, and has shown the ability and motivation to contribute fundamentally to the goals of the parent grant. The theme of the parent grant is the study of R-M systems that comprise the innate immune system in bacteria and archaea. A new paradigm in the in R-M field is the co-option of orphan and R-M methyltransferases in bacteria for epigenomic regulation of genes that are often associated with virulence factors. Richard proposes to study DNA methyltransferases (BCAM09902 and BCAL3494) from B.cenocepacia that regulate the bacterium’s biofilm formation or motility, as an extension of the parent grant. The research training is designed to bring him to a stage where he can take a problem from its infancy to an atomic crystal structure, with an understanding of the underlying thermodynamics and kinetics. The training plan has additional important elements, including specific courses and directed reading, participation in seminars and meetings, and an advisory committee to monitor and guide his progress. The expectation from this comprehensive training plan is that he will be able to successfully defend an outstanding PhD thesis, publish papers in a top tier journal(s), and also write a postdoctoral fellowship application for the next stage of his career in developing as an independent academic investigator.

项目摘要/摘要 该申请是要求多样性补充的要求，以培训和支持有才华的博士生，先生。 Richard Quintana-Feliciano，以补充我们的父母赠款（NIGMS R35-GM131780），标题为“结构和 限制修改（R-M）系统的特异性”。作为西班牙裔第一代学生 从社会经济地位低下，理查德一直在努力在他的学术准备的每一步中表现出色， 并表明了为父母赠款的目标做出根本贡献的能力和动力。 父母赠款的主题是完成细菌先天免疫系统的R-M系统的研究 和古细菌。在R-M领域中的一个新范式是孤儿和R-M甲基转移酶的共同选择 通常与病毒因子相关的基因表观基因组调节细菌。理查德 从b.cenocepacia研究DNA甲基转移酶（BCAM09902和BCAL3494）的提案 细菌的生物膜形成或运动性，作为父授予的扩展。研究培训是 旨在将他带到一个可以从婴儿期到原子晶体结构的阶段， 了解潜在的热力学和动力学。培训计划还有其他 重要的要素，包括特定课程和定向阅读，参与半人数和会议， 以及一个咨询委员会，以指导和指导他的进步。这个全面的期望 培训计划是，他将能够成功捍卫出色的博士学位论文，在顶部发表论文 Tier Journal（S），还为他的下一阶段的职业生涯撰写博士后奖学金申请 发展为独立的学术研究员。