Structure and Specificity of Restriction-Modification (R-M) Systems

限制性修饰（R-M）系统的结构和特异性

• 批准号: 10599570
• 负责人: ANEEL K. AGGARWAL
• 金额: $ 9.67万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10599570
• 关键词: Advisory Committees; Archaea; Bacteria; Burkholderia cepacia; Complement; Crystallization; DNA Modification Methylases; DNA Restriction-Modification Enzymes; Doctor of Philosophy; Elements; Fellowship; First Generation College Students; Gene Expression Regulation; Goals; Hispanic; Innate Immune System; Journals; Kinetics; Knowledge; Lead; Methyltransferase; Microbial Biofilms; Modification; Monitor; Motivation; National Institute of General Medical Sciences; Orphan; Paper; Pathogenicity; Publishing; Reading; Research Personnel; Research Training; Specificity; Structure; Talents; Thermodynamics; Training; Training Support; Virulence Factors; Writing; academic preparation; career; cell motility; design; doctoral student; epigenomics; expectation; infancy; low socioeconomic status; meetings; novel strategies; parent grant; underrepresented minority student

Project Summary/Abstract This application is a request for a Diversity Supplement to train and support a talented PhD student, Mr. Richard Quintana-Feliciano, to complement our parent grant (NIGMS R35-GM131780), titled “Structure and specificity of restriction-modification (R-M) systems”. As a Hispanic first-generation student, and coming from a low socioeconomic status, Richard has strived to excel at every step of his academic preparation, and has shown the ability and motivation to contribute fundamentally to the goals of the parent grant. The theme of the parent grant is the study of R-M systems that comprise the innate immune system in bacteria and archaea. A new paradigm in the in R-M field is the co-option of orphan and R-M methyltransferases in bacteria for epigenomic regulation of genes that are often associated with virulence factors. Richard proposes to study DNA methyltransferases (BCAM09902 and BCAL3494) from B.cenocepacia that regulate the bacterium’s biofilm formation or motility, as an extension of the parent grant. The research training is designed to bring him to a stage where he can take a problem from its infancy to an atomic crystal structure, with an understanding of the underlying thermodynamics and kinetics. The training plan has additional important elements, including specific courses and directed reading, participation in seminars and meetings, and an advisory committee to monitor and guide his progress. The expectation from this comprehensive training plan is that he will be able to successfully defend an outstanding PhD thesis, publish papers in a top tier journal(s), and also write a postdoctoral fellowship application for the next stage of his career in developing as an independent academic investigator.

项目概要/摘要 该申请是一项多样性补充申请，旨在培训和支持才华横溢的博士生，先生。 Richard Quintana-Feliciano，补充我们的家长补助金 (NIGMS R35-GM131780)，题为“结构和 限制修改（R-M）系统的特异性”。作为一名西班牙裔第一代学生，以及即将到来的。 理查德的社会经济地位较低，但他努力在学术准备的每一步都表现出色， 并表现出为实现家长补助金目标做出根本性贡献的能力和动力。 家长资助的主题是研究构成细菌先天免疫系统的 R-M 系统 R-M 领域的一个新范例是孤儿和 R-M 甲基转移酶的共同选择。 细菌对通常与毒力因子相关的基因进行表观基因组调控。 提议研究来自 B.cenocepacia 的 DNA 甲基转移酶（BCAM09902 和 BCAL3494），其调节 细菌的生物膜形成或运动，作为母体资助的延伸。 旨在把他带到一个阶段，使他能够将问题从婴儿期转化为原子晶体结构， 了解基本的热力学和动力学。培训计划还有其他内容。 重要要素，包括具体课程和定向阅读、参加研讨会和会议， 以及一个监督和指导他的进展的咨询委员会。 培养计划是他将能够成功答辩一篇优秀的博士论文，在顶级期刊上发表论文 级别期刊，并为他职业生涯的下一阶段撰写博士后奖学金申请 发展成为一名独立的学术研究者。