Structure and Specificity of Restriction-Modification (R-M) Systems

限制性修饰（R-M）系统的结构和特异性

• 批准号: 10797690
• 负责人: ANEEL K. AGGARWAL
• 金额: $ 11.79万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10797690
• 关键词: Cryoelectron Microscopy; DNA Binding; DNA Restriction-Modification Enzymes; Goals; Monitor; National Institute of General Medical Sciences; RNA-Binding Proteins; Services; Specificity; Structure; System; Vendor; X-Ray Crystallography; aged; fast protein liquid chromatography; instrument; parent grant; protein purification

Abstract Under the parent grant NIGMS R35-GM131780, titled “Structure and specificity of restriction-modification (R-M) systems”, this application is a supplemental request to replace an aged FPLC system that has been discontinued and is no longer supported and serviced by the vendor. All of the structural studies performed under the parent grant are heavily reliant on protein purification – a necessary prerequisite for X-ray crystallography and cryo-EM. The request is for an AKTA pure 25 M system with a Micro kit that will be 1) fully supported and serviced by the vendor, 2) allow the purification of proteins to be monitored at more than one wavelength, which is important because the aims of the parent grant are centered on DNA and RNA binding proteins, and 3) permit the purification of proteins on a microscale, which is essential, especially as the aims of the parent grant continue to grow in the direction of cryo-EM.

抽象的 根据母基金 NIGMS R35-GM131780，标题为“限制修改的结构和特异性” (R-M) 系统”，此申请是替换已老化的 FPLC 系统的补充请求 供应商已停止提供并不再提供所有结构研究的支持和服务。 在家长资助下，严重依赖蛋白质纯化——这是 X 射线的必要先决条件 要求使用带有 Micro 套件的 AKTA pure 25 M 系统 1) 得到供应商的充分支持和服务，2) 允许在超过 一个波长，这一点很重要，因为家长资助的目标集中在 DNA 和 RNA 结合蛋白，3) 允许在微尺度上纯化蛋白质，这是至关重要的，特别是当 家长资助的目标继续朝着冷冻电镜方向发展。