Mechanism of Meissner corpuscle function

迈斯纳小体功能机制

基本信息

批准号：
10597159
负责人：
Sviatoslav Bagriantsev
金额：
$ 48.95万
依托单位：
YALE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-04-01 至 2027-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10597159
关键词：
3-Dimensional Action Potentials Affect Afferent Neurons Biophysics Cells Communication Data Dense Core Vesicle Detection Development Ducks Electrophysiology (science)Elements Embryo Environment Exocytosis Food Generations Goals Histologic Histology Human Hypersensitivity Image Individual Ion Channel Ion Channel Gating Knowledge Life Light Mammals Mechanics Mechanoreceptors Mediating Meissners Corpuscle Modeling Molecular Morphology Mus Neurons Organ Pain Pharmacology Physiological Physiology Piezo 2 ion channel Play Population Process Role SNAP receptor Sensory Shapes Skin Social Behavior Social Development Somatosensory Disorders Specialist Stimulus Structure Tactile Testing Touch sensation Vertebrates density detector electron tomography experience experimental study insight knock-down mechanical signal mechanical stimulus neurophysiology optogenetics pharmacologic pleasure presynaptic reconstruction response somatosensory tool transcriptome

项目摘要

Somatosensation is essential for everyday life. Many vertebrates, including humans, use the sense of touch to navigate in the environment, manipulate tools and objects, find food and perform various mechanical tasks. Key to this ability are somatosensory corpuscles located in the skin. In this proposal, we focus on Meissner corpuscles, which are essential for performing fine tactile tasks due to their ability to detect transient mechanical stimuli. Despite their importance for somatosensation, the mechanism of Meissner corpuscle function is unknown. Meissner corpuscles are located in the skin of many vertebrates, and consist of lamellar cells in contact with a neuronal mechanoreceptor. The mechanoreceptor is thought to be the key sensory element within the corpuscle, whereas the function of lamellar cells is unknown. In this proposal, we will functionally test the hypothesis that Meissner lamellar cells are active detectors of touch. To do this, we will use bill skin of tactile specialist ducks, which contains a high density of Meissner corpuscles accessible for experimental manipulations. We will investigate the molecular mechanism of touch sensitivity in Meissner lamellar cells, study their interaction with neuronal mechanoreceptor, and investigate their role in somatosensory physiology. We will employ an integrative approach, including neurophysiology, biophysics and imaging. Results from this basic scientific proposal will provide mechanistic insights into the process of somatosensation in vertebrate skin.

节感对于日常生活至关重要。许多脊椎动物，包括人类，都使用触摸感在环境中导航，操纵工具和物体，找到食物并执行各种机械任务。钥匙具有这种能力的是位于皮肤中的体感体体。在此提案中，我们专注于Meissner 小体，这对于执行精细触觉任务至关重要，因为它们能够检测瞬态机械刺激。尽管它们对节感敏化的重要性，但Meissner Colduscle功能的机制是未知。 Meissner菌体位于许多脊椎动物的皮肤中，由接触的层状细胞组成带有神经元机械感受器。机械感受器被认为是小体，而层状细胞的功能尚不清楚。在此建议中，我们将在功能上测试假设Meissner层状细胞是触摸的主动探测器。为此，我们将使用触觉的比尔皮肤专业鸭子，其中包含高密度的Meissner小体，可用于实验操纵。我们将研究Meissner层状细胞中触摸敏感性的分子机理，研究它们与神经元机械感受器的相互作用，并研究它们在体感生理中的作用。我们将采用一种综合方法，包括神经生理学，生物物理学和成像。从这个基本的结果科学建议将提供有关脊椎动物皮肤透明效过程的机械见解。