Mechanism of touch sensitivity in rapidly-adapting mechanosensory corpuscles

快速适应机械感觉小体的触觉敏感性机制

基本信息

批准号：
10362938
负责人：
Sviatoslav Bagriantsev
金额：
$ 48.05万
依托单位：
YALE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-12-01 至 2027-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10362938
关键词：
Action Potentials Agreement Bioinformatics Cells Data Detection Development Ducks Electrophysiology (science)Elements Embryo Environment Generations Glean Histology Human Individual Ion Channel Ion Channel Gating Mammals Mechanical Stimulation Mechanics Mechanoreceptors Mediating Meissners Corpuscle Methods Modeling Molecular Morphology Neurons Organ Organism Pacinian Corpuscles Pain Pathologic Perception Pharmacology Physiological Physiological Processes Physiology Piezo 2 ion channel Play Population Process Role Shapes Skin Specialist Structure Tactile Temperature Testing Touch sensation Vertebrates biophysical analysis density experience experimental study insight knock-down mechanical force mechanical properties mechanical stimulus mechanotransduction nervous system disorder object recognition optogenetics parent grant pleasure pressure response sensor tool vibration voltage

Action Potentials Agreement Bioinformatics Cells Data Detection Development Ducks Electrophysiology (science)Elements Embryo Environment Generations Glean Histology Human Individual Ion Channel Ion Channel Gating Mammals Mechanical Stimulation Mechanics Mechanoreceptors Mediating Meissners Corpuscle Methods Modeling Molecular Morphology Neurons Organ Organism Pacinian Corpuscles Pain Pathologic Perception Pharmacology Physiological Physiological Processes Physiology Piezo 2 ion channel Play Population Process Role Shapes Skin Specialist Structure Tactile Temperature Testing Touch sensation Vertebrates biophysical analysis density experience experimental study insight knock-down mechanical force mechanical properties mechanical stimulus mechanotransduction nervous system disorder object recognition optogenetics parent grant pleasure pressure response sensor tool vibration voltage

展开

项目摘要

The ability to sense the world though physical contact is essential for all living organisms. In vertebrates, transient touch and vibration are detected by Pacinian corpuscles, but the mechanism of their function is poorly understood. Pacinian corpuscles are innervated by rapidly-adapting mechanoreceptor afferents, which detect touch due to expression of mechanically activated ion channels. The mechanoreceptor is surrounded by inner core cells, which are thought to provide auxiliary non-sensory support for the mechanoreceptor. Here, we will functionally test the hypothesis that inner core cells of Pacinian corpuscles are active touch sensors. We seek to reveal the molecular mechanism of touch sensitivity and touch-evoked excitability in inner core cells, and determine the effect of inner core cell activation on mechanoreceptor function. To do this, we will use bill skin of tactile specialist ducks, which contains a high density of Pacinian corpuscles accessible for electrophysiological manipulations. Because Pacinian corpuscles are present in the skin or internal organs of most vertebrates, including all mammals, our results will further our understanding of general molecular and cellular mechanisms of touch detection.

尽管物理接触对世界来说，感知世界的能力对于所有生物体都是必不可少的。在脊椎动物中，瞬态触摸和振动是通过Pacinian Copuscles检测到的，但其功能的机制很差理解。 Pacinian小体是通过快速适应机械感受器传入来支配的，该传入检测由于机械激活的离子通道的表达而触摸。机械感受器被内部包围核心细胞被认为可以为机械感受器提供辅助非敏感性支持。在这里，我们会的在功能上测试了Pacinian体体的内核细胞是主动触摸传感器的假设。我们寻找揭示触摸敏感性和触摸感触点的分子机制，并在内部核心细胞中触摸诱发的兴奋性，以及确定内核细胞激活对机械感受器功能的影响。为此，我们将使用账单皮肤触觉专家鸭，其中包含高密度的Pacinian小体，可用于电生理学操纵。由于大多数脊椎动物的皮肤或内部器官都存在于Pacinian小体，所以包括所有哺乳动物，我们的结果将进一步了解一般分子和细胞机制触摸检测。