Microbiology, Immunology, Animal Modeling and Imaging

微生物学、免疫学、动物建模和成像

• 批准号: 10593152
• 负责人: WILLIAM Ramses BISHAI
• 金额: $ 14.07万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-03-16 至 2027-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10593152
• 关键词: Accountability; Animal Housing; Animal Model; Animals; Basic Science; Biological; Breeding; Cavia; Clinic; Computer software; Data; Data Analyses; Data Storage and Retrieval; Dedications; Disease; Environmental Exposure; Equipment; FAIR principles; Faculty; Feedback; Flow Cytometry; Goals; Grant; HIV/TB; Histopathology; Hormones; Image; Imaging Device; Immune response; Immunologics; Immunology; Infection; Inflammation; Infrastructure; Institution; Laboratories; Leadership; Measurement; Mentors; Methodology; Methods; Microbiology; Mission; Modeling; Molecular Genetics; Mus; Mycobacterium tuberculosis; Oryctolagus cuniculus; Outcome Measure; Pathogenesis; Persons; Positron-Emission Tomography; Predisposition; Preparation; Privatization; Procedures; Protocols documentation; Research; Research Personnel; Resolution; Scanning; Schedule; Series; Services; Sex Differences; Site; Span 20; Speed; Standardization; Techniques; Technology; Tissue Sample; Training; Translating; Tuberculosis; United States National Institutes of Health; Universities; Writing; animal breeding; animal imaging; clinically relevant; co-infection; cytokine; data sharing; experience; imager; imaging approach; imaging study; implantation; in vivo imaging; laboratory equipment; meetings; metabolomics; next generation; novel; pre-clinical; preclinical study; primary endpoint; programs; radiotracer; reconstruction; research study; response; single photon emission computed tomography; single-cell RNA sequencing; synergism; tool; training opportunity

ABSTRACT – JHU TRAC Microbiology, Immunology, Animal Modeling and Imaging (MIAMI) Core The Johns Hopkins University (JHU) TRAC Microbiology, Immunology, Animal Modeling and Imaging (MIAMI) Core, comprising laboratories primarily within the Johns Hopkins Center for Tuberculosis Research (CTR) and the Center for Infection and Inflammation Imaging Research (Ci3R), will offer a broad panel of laboratory-based tools to support the mission of the JHU TRAC. Headed by Co-Directors Drs. William Bishai and Sanjay Jain and assisted by a Core faculty of 10 additional highly accomplished tuberculosis (TB) and non-TB investigators, the MIAMI Core will provide decades of combined research and mentoring expertise, as well as access to cutting-edge laboratory technologies to enable training opportunities in and support for TB research in: (a) Microbiology, molecular genetics and biosafety level-3 (BSL3) handling of Mycobacterium tuberculosis; (b) Immunologic tools to assess host responses to TB infection and diseases; and (c) Multiple clinically-relevant animal models of TB (mouse, guinea pig, and rabbit), alongside the capacity to conduct in vivo imaging with CT, PET, and SPECT. The MIAMI Core will assist New Investigators (NI) and Early Stage Investigators (ESI) in planning, executing and interpreting the data from all preclinical studies, including small animal models of TB, mouse breeding, advanced whole-body live in vivo imaging approaches, metabolomics, and single-cell RNA (scRNA) sequencing. In addition to standard aspects of laboratory-based research on M. tuberculosis microbiology, immunology and pathogenesis, the MIAMI core will provide added value by offering novel topics (e.g., TB-HIV co-infection, environmental exposures and TB susceptibility, sex differences in TB susceptibility, and advanced animal imaging), which are not currently available through the CTR and are unique to the JHU TRAC. Furthermore, we will leverage numerous independent and institutional grants to further strengthen basic science capabilities within the JHU TRAC. The MIAMI Core staff will provide scientific and technical leadership and operational oversight to all animal- related studies of the research program. MIAMI Core faculty will also participate in the weekly TRAC Seminar Series, providing a scientific focus on novel research in TB pathogenesis and novel imaging tools, as well as the TRAC-sponsored Writing Accountability Groups for NI/ESI, TRAC K-Club and K2R Club, and annual TB Scientific Meeting and TRAC Data Fair. A centralized Core is essential to promote synergy with the other JHU TRAC Cores and to provide integration, efficiency, and ease of use that will maximize value and utility for all junior and established Investigators involved in the JHU TRAC. Overall, the MIAMI Core will add significant value through integration of expertise, infrastructure, and capabilities to achieve the goal of providing world-class microbiology, immunology, animal models, and imaging support to the JHU TRAC, as well as research opportunities to trainees, junior faculty, and researchers new to TB.

摘要 - JHU TRAC微生物学，免疫学，动物建模和成像（迈阿密）核心 约翰·霍普金斯大学（JHU）TRAC微生物学，免疫学，动物建模和成像 （迈阿密）核心，主要在约翰·霍普金斯结核病中心内完成实验室 研究（CTR）和感染与炎症成像研究中心（CI3R）将提供广泛的 基于实验室的工具，以支持JHU TRAC的任务。由联合导演Drs领导。 威廉·比舍（William Bishai）和桑杰·贾恩（Sanjay Jain），并获得了另外10个高度成就的核心教师 结核病（TB）和非TB研究人员，迈阿密核心将提供数十年的合并研究 和心理专业知识，以及获得尖端实验室技术以实现培训 （a）微生物学，分子遗传学和生物安全级别3 （BSL3）处理结核分枝杆菌； （b）评估宿主反应的免疫学工具 感染和疾病； （c）TB的多个临床上与临床上相关的动物模型（小鼠，豚鼠和 兔子），以及与CT，PET和SPECT进行体内成像的能力。 迈阿密核心将协助新调查人员（NI）和早期研究人员（ESI）计划， 执行和解释来自所有临床前研究的数据，包括TB的小动物模型 繁殖，高级全身现场体内成像方法，代谢组学和单细胞RNA （SCRNA）测序。除了基于实验室的结核分枝杆菌研究的标准方面 微生物学，免疫学和发病机理，迈阿密核心将通过提供新颖的新价值 主题（例如TB-HIV共同感染，环境暴露和结核病敏感性，结核病的性别差异 敏感性和先进的动物成像），目前尚未通过CTR可用，并且是 Jhu Trac独有的。此外，我们将利用众多独立和机构赠款来 进一步增强了JHU TRAC内的基础科学能力。 迈阿密核心人员将为所有动物提供科学和技术领导和运营监督 - 研究计划的相关研究。迈阿密核心教师还将参加每周的TRAC 研讨会系列，科学的关注于TB发病机理和新型成像工具的新研究， 以及NI/ESI，TRAC K-CLUB和K2R俱乐部的TRAC赞助的写作问责小组， 以及年度结核病科学会议和TRAC数据博览会。集中核对于促进协同作用至关重要 使用其他JHU TRAC核心，并提供一体化，效率和易用性，以最大程度地提高 参与JHU TRAC的所有初级和已建立的研究人员的价值和实用性。总体而言，迈阿密 核心将通过整合专业知识，基础架构和实现能力来增加重大价值 提供世界一流的微生物学，免疫学，动物模型和成像支持的目的 JHU TRAC，以及向学员，初级教职员工和TB的研究人员提供的研究机会。