Bat pluripotent stem cells as a novel experimental system

蝙蝠多能干细胞作为一种新型实验系统

基本信息

批准号：
10592777
负责人：
Thomas P. Zwaka
金额：
$ 25.35万
依托单位：
ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-08-14 至 2025-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10592777
关键词：
Address Affect Animal Model Anti-Inflammatory Agents Biological Models Biology Breeding COVID-19 CRISPR/Cas technology Cell Differentiation process Cell Line Cell model Cells Chiroptera Clustered Regularly Interspaced Short Palindromic Repeats Complex Data Ebola Echolocation Engineering Enzymes Evolution Genes Genetic study Goals Immune Immune Tolerance Immune response Immune system Immunity In Vitro Inflammasome Inflammatory Inflammatory Response Knock-out Longevity Mammals Metabolic Metabolism Modeling Molecular Myeloid Cells Natural Immunity Organoids Outcome Pathway interactions Physiology Play Pluripotent Stem Cells Property Protocols documentation Radiation Readiness Research Research Proposals Role Sampling Series Severe Acute Respiratory Syndrome System Testing Tissues Viral load measurement Virus Virus Diseases Work cell immortalization cell type cellular pathology fascinate fly forward genetics genetic testing healthspan induced pluripotent stem cell insight ketogenesis ketogentic marenostrin metabolomics model organism novel programs response stem cells tool transcriptomics tumorigenesis viral outbreak

项目摘要

Bats are perhaps the most fascinating but least understood mammalian order. It is clear that, although bats are a critically needed new model organism, limited access to animal and cell models has hindered their study. We recently generated the first induced pluripotent stem cells (iPSCs) from a bat (R. ferrumequinum) and discovered that bat iPSCs had a unique transcriptomic profile enriched in metabolomic and immune pathways ketogenic metabolism and a unique inflammasome response. We plan to generate additional to test if our novel protocol can be used for any bat species and that essential metabolic genes uniquely expressed only in bat cells, including HMGC2, can be knocked out using CRISPR/Cas9. We will also evaluate if the inflammatory response is altered in myeloid cells derived from bat iPSCs. Bat stem cell lines and differentiated progeny will help address bat physiology’s most tantalizing questions, including immunity.

很明显，蝙蝠可能是最令人着迷但最不为人所知的哺乳动物目。尽管蝙蝠是一种急需的新模式生物，但获得动物的机会有限细胞模型阻碍了他们的研究。来自蝙蝠（R. ferrumequinum）的多能干细胞（iPSC），并发现蝙蝠 iPSC 具有独特的转录组学特征，富含代谢组学和免疫学特征我们计划研究生酮途径代谢和独特的炎症反应。生成额外的内容来测试我们的新协议是否可以用于任何蝙蝠物种，并且仅在蝙蝠细胞中独特表达的重要代谢基因，包括 HMGC2，可以我们还将评估炎症反应是否会被使用 CRISPR/Cas9 敲除。源自蝙蝠 iPSC 的骨髓细胞发生改变并分化。后代将有助于解决蝙蝠生理学中最诱人的问题，包括免疫。