COBRE on Membrane Protein Production and Characterization

COBRE 膜蛋白生产和表征

基本信息

批准号：
8141171
负责人：
ABRAHAM M LENHOFF
金额：
$ 113.49万
依托单位：
UNIVERSITY OF DELAWARE
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-09-10 至 2015-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8141171
关键词：
COBRE Membrane Protein Production Characterization

项目摘要

DESCRIPTION (provided by applicant): We propose to continue the COBRE on Membrane Protein Production and Characterization at the University of Delaware as a Phase III COBRE infrastructure center. The critical role of membrane proteins in biology is clearly reflected in the fact that they represent >30% of the human genome and are the targets of >50% of all therapeutics on the market today. Nevertheless, significant challenges remain in producing and characterizing them, especially within the realm of structural biology. Consequently the objectives of research in the COBRE will be to express, solubilize, purify and crystallize membrane proteins; to determine their structures; and to characterize their functions at the molecular level and in larger biological systems in both health and disease. These objectives are congruent with those of the Structural Biology component of the NIH Roadmap and the Protein Structure Initiative in addressing an area of critical importance for biomedical research. The infrastructure center will complement research in the laboratories of an interdisciplinary group of more than a dozen Pis, whose work is independently supported. To this end the center will include: research and instrumentation cores (Protein Expression and Biophysical Characterization; Structural Biology; and a group of Core Facilities co-funded with, among others, the Delaware INBRE); continuation of the current pilot research subproject program; a predoctoral training program modeled on the NIH T32 mechanism; and continuation of the existing annual symposium series. The administration of the center will be guided by an internal Steering Committee and will continue to benefit from the input of an External Advisory Committee, including for review of pilot project proposals. The research within the COBRE coupled with the capabilities provided by the infrastructure center will continue to give the University of Delaware distinctive strength in an area of research critical to biology and biomedicine. PUBLIC HEALTH RELEVANCE (provided by applicant): Membrane proteins exist in the membrane surrounding each cell and are critical to cellular growth, communication and nutrition, yet how these proteins function is still relatively poorly understood. The purpose of this center is to provide infrastructure for research to develop improved methods to produce and study membrane proteins, which can yield major benefits for basic biology and drug development.

描述（由申请人提供）：我们建议在特拉华大学继续开展膜蛋白生产和表征方面的 COBRE，作为第三阶段 COBRE 基础设施中心。膜蛋白在生物学中的关键作用清楚地反映在以下事实中：它们代表了超过 30% 的人类基因组，并且是当今市场上超过 50% 的治疗药物的靶标。然而，在生产和表征它们方面仍然存在重大挑战，特别是在结构生物学领域。因此，COBRE 的研究目标是表达、溶解、纯化和结晶膜蛋白；确定它们的结构；并在分子水平和更大的生物系统中表征它们在健康和疾病方面的功能。这些目标与 NIH 路线图的结构生物学部分和蛋白质结构计划的目标一致，以解决生物医学研究至关重要的领域。该基础设施中心将补充由十多个 Pi 组成的跨学科小组的实验室研究，该小组的工作得到独立支持。为此，该中心将包括：研究和仪器核心（蛋白质表达和生物物理表征；结构生物学；以及与特拉华州 INBRE 等共同资助的一组核心设施）；继续当前的试点研究子项目计划；仿照 NIH T32 机制的博士前培训计划；以及现有年度研讨会系列的延续。该中心的管理将由内部指导委员会指导，并将继续受益于外部咨询委员会的投入，包括审查试点项目提案。 COBRE 内的研究加上基础设施中心提供的能力将继续赋予特拉华大学在生物学和生物医学关键研究领域的独特优势。公共健康相关性（由申请人提供）：膜蛋白存在于每个细胞周围的膜中，对细胞生长、通讯和营养至关重要，但人们对这些蛋白如何发挥作用仍然知之甚少。该中心的目的是为研究提供基础设施，以开发改进的方法来生产和研究膜蛋白，这可以为基础生物学和药物开发带来重大益处。

项目成果

期刊论文数量（24）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Ligand-dependent cholesterol interactions with the human A(2A) adenosine receptor.

配体依赖性胆固醇与人类 A(2A) 腺苷受体的相互作用。

DOI：
发表时间：
2013-04
期刊：
影响因子：
3.4
作者：
Lee, Ji Young;Patel, Rohan;Lyman, Edward
通讯作者：
Lyman, Edward

Expression and purification of the membrane enzyme selenoprotein K.

膜酶硒蛋白K的表达和纯化。

DOI：
10.1016/j.pep.2012.08.014
发表时间：
2012-11-01
期刊：
Protein expression and purification
影响因子：
1.6
作者：
Jun Liu;P. Srinivasan;D. N. Pham;S. Rozovsky
通讯作者：
S. Rozovsky

Three-dimensional structure of CAP-gly domain of mammalian dynactin determined by magic angle spinning NMR spectroscopy: conformational plasticity and interactions with end-binding protein EB1.

通过魔角旋转核磁共振波谱测定哺乳动物动力蛋白 CAP-gly 结构域的三维结构：构象可塑性以及与末端结合蛋白 EB1 的相互作用。

DOI：
发表时间：
2013-11-15
期刊：
影响因子：
5.6
作者：
Yan, Si;Hou, Guangjin;Schwieters, Charles D;Ahmed, Shubbir;Williams, John C;Polenova, Tatyana
通讯作者：
Polenova, Tatyana

Improved protease digestion conditions for membrane protein detection.

改进了膜蛋白检测的蛋白酶消化条件。