COBRE on Membrane Protein Production and Characterization

COBRE 膜蛋白生产和表征

基本信息

批准号：
8726442
负责人：
ABRAHAM M LENHOFF
金额：
$ 110.29万
依托单位：
UNIVERSITY OF DELAWARE
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-09-10 至 2016-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8726442
关键词：
Address Adopted Advisory Committees Area Biological Process Biology Biomedical Research Cells Centers of Research Excellence Communication Communities Complement Complex Core Facility Core Protein Coupled Crystallization Data Delaware Detergents Development Disease Faculty FarGo Funding Genomics Health Human Genome Institutional National Research Service Award Laboratories Lead Marketing Membrane Membrane Proteins Methods Modeling Molecular Phase Pilot Projects Production Protein Structure Initiative Proteins Research Research Infrastructure Research Personnel Research Support Role Series Structure Therapeutic Training Programs United States National Institutes of Health Universities Work biological systems biophysical properties cell growth drug development improved infrastructure development instrumentation interest nutrition outreach overexpression pre-doctoral programs protein expression protein function protein structure solid state nuclear magnetic resonance structural biology symposium

项目摘要

We propose to continue the COBRE on Membrane Protein Production and Characterization at the University of Delaware as a Phase III COBRE infrastructure center. The critical role of membrane proteins in biology is clearly reflected in the fact that they represent >30% of the human genome and are the targets of >50% of all therapeutics on the market today. Nevertheless, significant challenges remain in producing and characterizing them, especially within the realm of structural biology. Consequently the objectives of research in the COBRE will be to express, solubilize, purify and crystallize membrane proteins; to determine their structures; and to characterize their functions at the molecular level and in larger biological systems in both health and disease. These objectives are congruent with those of the Structural Biology component of the NIH Roadmap and the Protein Structure Initiative in addressing an area of critical importance for biomedical research. The infrastructure center will complement research in the laboratories of an interdisciplinary group of more than a dozen PIs, whose work is independently supported. To this end the center will include: research and instrumentation cores (Protein Expression and Biophysical Characterization; Structural Biology; and a group of Core Facilities co-funded with, among others, the Delaware INBRE); continuation of the current pilot research subproject program; a predoctoral training program modeled on the NIH T32 mechanism; and continuation of the existing annual symposium series. The administration of the center will be guided by an internal Steering Committee and will continue to benefit from the input of an External Advisory Committee, including for review of pilot project proposals. The research within the COBRE coupled with the capabilities provided by the infrastructure center will continue to give the University of Delaware distinctive strength in an area of research critical to biology and biomedicine.

我们建议继续开展关于膜蛋白生产和表征的 COBRE 特拉华大学作为第三期 COBRE 基础设施中心。膜蛋白的关键作用在生物学中的重要性清楚地反映在以下事实：它们代表了人类基因组的 30% 以上，并且是目标占当今市场上所有疗法的 50% 以上。然而，生产方面仍然存在重大挑战并对它们进行表征，特别是在结构生物学领域。因此，目标 COBRE 的研究将是表达、溶解、纯化和结晶膜蛋白；确定它们的结构；并在分子水平和更大的生物系统中表征它们的功能健康和疾病。这些目标与结构生物学部分的目标一致 NIH 路线图和蛋白质结构倡议在解决一个至关重要的领域生物医学研究。该基础设施中心将补充由更多人组成的跨学科小组实验室的研究十几位 PI，他们的工作得到独立支持。为此，该中心将包括：研究和仪器核心（蛋白质表达和生物物理表征；结构生物学；以及一组与特拉华州 INBRE 等共同资助的核心设施）；继续当前的试点研究子项目计划；仿照 NIH T32 机制的博士前培训计划；和现有年度研讨会系列的延续。该中心的管理将由内部指导委员会并将继续受益于外部咨询委员会的投入，包括审查试点项目提案。 COBRE 内的研究与能力相结合基础设施中心提供的服务将继续赋予特拉华大学在以下领域的独特优势：对生物学和生物医学至关重要的研究领域。

项目成果

期刊论文数量（13）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Thermoresponsive self-assembly of nanostructures from a collagen-like peptide-containing diblock copolymer.

含胶原蛋白肽的二嵌段共聚物纳米结构的热响应自组装。

DOI：
10.1002/mabi.201400358
发表时间：
2015-01
期刊：
Macromolecular bioscience
影响因子：
4.6
作者：
Luo T;He L;Theato P;Kiick KL
通讯作者：
Kiick KL

Self-Assembly of Stable Nanoscale Platelets from Designed Elastin-like Peptide-Collagen-like Peptide Bioconjugates.

由设计的类弹性蛋白肽-类胶原肽生物缀合物自组装稳定的纳米级血小板。

DOI：
10.1021/acs.biomac.8b01681
发表时间：
2019-02-21
期刊：
Biomacromolecules
影响因子：
6.2
作者：
Jingya Qin;Tianzhi Luo;K. Kiick
通讯作者：
K. Kiick

⁵¹V NMR Crystallography of Vanadium Chloroperoxidase and Its Directed Evolution P395D/L241V/T343A Mutant: Protonation Environments of the Active Site.

钒氯过氧化物酶的 V NMR 晶体学及其定向进化 P395D/L241V/T343A 突变体：活性位点的质子化环境。

DOI：
发表时间：
2015-04-29
期刊：
影响因子：
15
作者：
Gupta, Rupal;Hou, Guangjin;Renirie, Rokus;Wever, Ron;Polenova, Tatyana
通讯作者：
Polenova, Tatyana

Aggregates of α-chymotrypsinogen anneal to access more stable states.

α-胰凝乳蛋白酶原的聚集体退火以获得更稳定的状态。

DOI：
发表时间：
2014-04
期刊：
影响因子：
3.8
作者：
Maurer, Ronald W;Hunter, Alan K;Robinson, Anne S;Roberts, Christopher J
通讯作者：
Roberts, Christopher J

Auxin-dependent control of a plasmodesmal regulator creates a negative feedback loop modulating lateral root emergence.

胞间连丝调节因子的生长素依赖性控制产生了调节侧根出现的负反馈回路。

DOI：
发表时间：
2020
期刊：
影响因子：
16.6
作者：
Sager, Ross;Wang, Xu;Hill, Kristine;Yoo, Byung;Caplan, Jeffery;Nedo, Ale;Tran, Thu;Bennett, Malcolm J;Lee, Jung
通讯作者：
Lee, Jung