PPG Phenotyping

PPG表型分析

• 批准号: 10262916
• 负责人: Joseph L. Witztum
• 金额: $ 41.41万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-21 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10262916
• 关键词: Atherosclerosis; Cardiovascular system; Disease; Fibrosis; Gap Junctions; Histologic; Histology; Institutes; International; Laboratories; Lipids; Lipoprotein (a); Lipoproteins; Liver; Low-Density Lipoproteins; Maps; Measures; Methodology; Mission; Morphology; Mus; Pathology; Pathway interactions; Phenotype; Plasma; Preparation; Proteins; Proteomics; Reagent; Research Personnel; Sampling; Services; Standardization; Techniques; Technology; Tissues; chemokine; cytokine; data collection site; human subject; lipidomics; mouse model; nonalcoholic steatohepatitis; sample collection; synthetic polymer Bioplex

PROJECT SUMMARY Core A. Phenotyping The mission of the Phenotyping Core A is to provide standardized analytical methodologies and services to assist the Project Investigators in efficiently achieving the Scientific Aims of their respective projects. The Core will provide targeted phenotyping of both mouse models and human subjects, as well as providing selected reagents used by the Projects. The Core is organized into 5 Sub-Cores, which will provide the following services: 1) An Atherosclerosis Morphology Sub-Core to provide qualitative and quantitative analyses of mouse atherosclerosis. 2) Liver Morphology Sub-Core to provide qualitative and quantitative analysis of mouse liver histology in the context of NASH and fibrosis. 3) Targeted Lipidomic Analysis Sub-Core to provide analyses of plasma and tissues in the UCSD LIPID MAPS Lipidomic Core. 4) Targeted Proteomic Sub-Core to provide high- throughput, high- sensitivity analysis of cytokines, chemokines and other analytes with the use of Luminex Bio- Plex technology and 5). Lipoprotein Sub-Core, which will measure lipid and lipoprotein levels in mouse plasma and also provide standardized preparations of LDL and modified LDLs (and other needed lipoproteins) for investigators’ use. The Core will be centered and organized in Dr. Witztum's laboratory at UCSD, who will be the overall director and responsible to see that all of the Sub-Cores fulfill their missions. The Atherosclerosis Morphology and Liver Morphology Sub-Cores will be housed at UCSD under the Direction of Dr. Witztum with assistance of a UCSD expert in liver pathology (Dr. Tatiana Kisseleva), as will the services of the Lipoprotein Core. High- throughput selected proteomic type analyses of cytokines, chemokines and related proteins will be performed at the Salk Institute under the direction of Drs. Ron Evans and Michael Downes. Finally, the Lipid Maps Lipidomic Core, under the direction of Drs. Oswald Quehenberger and Ed Dennis is also located at UCSD in the same building as Dr. Witztum’s laboratories. Thus, all sample collection will be centralized in a central laboratory at UCSD, where they will be logged into an Excel spreadsheet to provide a central organizational pathway for distribution of samples to proper Sub-Cores and in turn, a central site for data collection of results.

项目摘要 核心A.表型 表型核心A的使命是提供标准化的分析方法和服务 协助项目调查人员有效地实现其各自项目的科学目标。核心 将提供小鼠模型和人类受试者的有针对性表型，并提供所选的 项目使用的试剂。核心组织为5个子核，将提供以下服务： 1）动脉粥样硬化的形态亚核核心，以提供小鼠的定性和定量分析 动脉粥样硬化。 2）肝脏形态子核心提供小鼠肝脏的定性和定量分析 在NASH和纤维化的背景下组织学。 3）靶向脂肪组分析子核心，以提供分析 UCSD脂质图中的血浆和组织。 4）靶向蛋白质组学子核可提供高 使用Luminex Bio-对细胞因子，趋化因子和其他分析物的高敏感性分析 PLEX技术和5）。脂蛋白亚核，它将测量小鼠血浆中的脂质和脂蛋白水平 并为LDL和改良的LDL（以及其他需要的脂蛋白）提供标准化的制剂 调查人员的使用。 该核心将以UCSD的Witztum博士的实验室为中心和组织，他们将成为整体 负责人并负责看到所有子核都履行了任务。动脉粥样硬化的形态 在Witztum博士的指导下，将在UCSD的指导下，并在Witztum博士的指导下，在 UCSD是肝脏病理学的专家（Tatiana Kisseleva博士），脂蛋白核心的服务也将是。高的- 吞吐量选定的细胞因子，趋化因子和相关蛋白质的蛋白质组学类型分析将在 Salk研究所在Drs的指导下。罗恩·埃文斯（Ron Evans）和迈克尔·唐斯（Michael Downes）。最后，脂质地图脂质组学 核心，在Drs的指导下。 Oswald Quehenberger和Ed Dennis也位于UCSD 作为Witztum博士的实验室建造。那，所有样本收集将集中在中央实验室 UCSD，它们将被登录到Excel电子表格，为中心的组织途径提供 将样品分布到适当的子核，然后又是结果数据收集结果的中心地点。