Same-day Breast Pathology Results using Open-Top Light-Sheet Microscopy for Image-guided Core Needle Biopsies

使用开顶光片显微镜进行图像引导核心针活检的当天乳腺病理学结果

基本信息

批准号：
10253425
负责人：
Nicholas Reder
金额：
$ 39.83万
依托单位：
ALPENGLOW BIOSCIENCES, INC.
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-07-01 至 2023-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10253425
关键词：
Accounting Address Anxiety Area Benign Biopsy Biopsy Specimen Blinded Breast Breast Cancer Detection Businesses Calendar Cancer Diagnostics Caring Categories Cessation of life Clinic Clinical Clinical Trials Communication Core Biopsy Correlation Studies Detection Diagnosis Diagnostic Diagnostic Imaging Disease Emotional Enrollment Ensure Evaluation Event Formalin Fresh Tissue Frozen Sections Frustration Gold Health Hematoxylin and Eosin Staining Method Histopathology Holidays Image Image Guided Biopsy Imaging Techniques Lead Lesion Letters Light Malignant - descriptor Malignant Neoplasms Mammary Gland Parenchyma Mammography Mastectomy Microscopy Operative Surgical Procedures Paraffin Embedding Pathologic Pathologist Pathology Patients Personal Satisfaction Persons Physical Examination Preparation Process Provider Radiology Specialty Reporting Sampling Scanning Schedule Screening Result Site Slide Specimen Stains Technology Telephone Testing Thinness Time Tissue Sample Tissues Transportation Triage Tumor-Infiltrating Lymphocytes Ultrasonography Wait Time Woman biomarker evaluation breast cancer diagnosis breast imaging breast lumpectomy breast pathology cancer care cancer diagnosis clinical Diagnosis clinical imaging fluorescence imaging image guided improved malignant breast neoplasm microscopic imaging novel prevent radiomics research clinical testing sample fixation screening

项目摘要

Abstract (300 words maximum) Each year, over 700 women undergo an image-guided core needle biopsy (CNB) to evaluate a suspicious breast finding at the Seattle Cancer Care Alliance, with only about 30% biopsies yielding malignancy. Typical turnaround time for determination of the presence or absence of cancer from CNBs is three to five business days, and this waiting time can lead to avoidable anxiety and delays in management. Our team has pioneered the use of open-top light-sheet microscopy (OTLS), which is a slide-free fluorescence imaging technique that enables rapid, large area scanning of fresh tissue. A preliminary study using excised 1 cm2 pieces of fresh breast tissue showed that OTLS imaging can provide diagnostic quality images in under 10 minutes. We hypothesize that OTLS evaluation of CNB breast tissue specimens will provide an accurate determination of malignancy vs. no malignancy within 30 minutes of obtaining breast core specimens. We will test this hypothesis by optimizing evaluation of CNB samples with OTLS using breast cancers that have been excised surgically and then testing its accuracy in a small clinical trial of 40 women. If successful, our approach could allow women with concerning breast imaging findings to have a complete imaging and pathology workup in the same day, leading to reassurance in the majority of women and earlier triage to appropriate care in those with breast cancer. Furthermore, wider application of this technology could allow for more accurate whole-specimen evaluation of biomarkers of disease, such as quantification of tumor infiltrating lymphocytes, and more elegant radiology-pathology correlation studies that could advance the emerging fields of radiomics and pathomics.

摘要（最多 300 字）每年，超过 700 名女性接受图像引导的空芯针活检 (CNB)，以评估西雅图癌症护理联盟发现可疑乳房，仅约 30% 进行了活检产生恶性肿瘤。确定是否存在的典型周转时间 CNB 的癌症需要三到五个工作日，这个等待时间可能会导致本可以避免的焦虑和管理延误。我们的团队率先使用开顶式光片显微镜 (OTLS)，这是一种无载玻片荧光成像技术，可实现快速、大面积扫描新鲜组织。使用切下的 1 cm2 新鲜切片进行初步研究乳腺组织表明 OTLS 成像可以在 10 秒内提供诊断质量的图像分钟。我们假设 CNB 乳腺组织标本的 OTLS 评估将在 30 分钟内准确确定恶性与非恶性获取乳房核心标本。我们将通过优化评估来检验这个假设 CNB 使用经过手术切除的乳腺癌进行 OTLS 采样，然后在一项由 40 名女性参与的小型临床试验中测试其准确性。如果成功的话，我们的方法可以让有相关乳腺影像学检查结果的女性需要进行完整的影像学和病理学检查在同一天进行检查，使大多数女性放心并尽早分诊对乳腺癌患者进行适当的护理。此外，该技术的广泛应用可以对疾病的生物标志物进行更准确的全样本评估，例如肿瘤浸润淋巴细胞的定量，以及更优雅的放射学-病理学相关性可以推进放射组学和病理组学新兴领域的研究。