Diversity of the Mosquito Microbiota and its Influence on Pathogen Transmission

蚊子微生物群的多样性及其对病原体传播的影响

• 批准号: 10237919
• 负责人: Matthew C I Medeiros
• 金额: $ 17.47万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-15 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10237919
• 关键词: Address; Aedes; Arboviruses; Biological Sciences; Blood; Chikungunya virus; Communities; Competence; Culex (Genus); Culicidae; Dengue; Dengue Virus; Development; Digestion; Disease; Fertility; Food; Genes; Genetic; Genotype; Hawaiian population; Health; Human; Immune system; Individual; Infection; Intervention; Knowledge; Laboratories; Larva; Link; Malaria; Medical; Metabolism; Microbe; Modeling; Mosquito-borne infectious disease; Nature; Outcome; Parasite resistance; Parasites; Phenotype; Population; Public Health; Research; Resource Sharing; Role; Shapes; Structure; Surveys; Temperature; Testing; Time; Variant; Vectorial capacity; Work; Zika Virus; base; density; design; differential expression; disease transmission; experimental study; frontier; human disease; human pathogen; life history; microbial; microbiome; microbiota; microbiota profiles; mosquito-borne pathogen; novel; novel strategies; paratransgenesis; pathogen; professor; response; symbiont; trait; translational impact; transmission process; vector; vector competence; vector mosquito

Project Summary – PROJECT 2: JI, Matthew Medeiros The symbiotic microbiota of mosquitoes has pervasive effects on host traits, including those of vectors of important human diseases like dengue virus, chikungunya virus, and Zika virus. Coordinated interactions between mosquitoes and their microbiota shape mosquito metabolism, development, fecundity, survival, and immune systems. These effects on individual vectors may scale to influence the transmission of infectious human pathogens at the population and community levels. While the mosquito microbiota is known to modulate the capacity of vectors to sustain human pathogen transmission, the factors that contribute to variation in the composition and density of the mosquito microbiota across individual vectors and vector populations remain poorly resolved. The central hypothesis of this proposal posits that (i) environmental, ecological, and genetic forces drive the assembly of the mosquito microbiota; (ii) differences in these forces over time and space produce variation in the microbiota between individual vectors; and, (iii) this variation between individual vectors scales to impact disease transmission among hosts, including humans. To test this hypothesis, this study will employ numerous carefully designed experiments in both field and controlled laboratory settings. These experiments aim to clarify the role of environmental, ecological, and genetic factors in shaping mosquito microbiota diversity using Hawaiian populations of two medically important vectors, Culex quinquefasciatus and Aedes albopictus. In addition, it will investigate the consequences of this diversity on dengue transmission, the most relevant mosquito-borne pathogen in Hawai`i. Expected outcomes of this work include a better model for the assembly of the mosquito microbiota and its cumulative impact on pathogen transmission. Ultimately, this study is expected to have a positive translational impact by informing the development of novel, microbe-based strategies to mitigate mosquito-borne diseases of public health concern.

项目摘要 - 项目2：JI，Matthew Medeiros 蚊子的共生微生物群对宿主特征具有普遍的影响，包括 重要的人类疾病，例如登革热病毒，基孔肯雅病毒和寨卡病毒。协调的互动 在蚊子及其微生物群之间形状的蚊子代谢，发育，生育，生存和 免疫系统。这些对单个向量的影响可能会扩展以影响传染性的传播 人口和社区水平的人类病原体。虽然已知蚊子微生物群 调节向量维持人病原体传播的能力，这些因素有助于 跨个体载体和载体的蚊子微生物群的组成和密度变化 人口的解决方案仍然很差。该提议的中心假设认为（i）环境， 生态和遗传力驱动蚊子菌群的组装； （ii）这些力的差异 随着时间和空间的生产变化，各个向量之间的微生物群；和（iii）这种变体 在各个矢量之间，影响包括人类在内的宿主之间的疾病传播。测试这个 假设，本研究将在田间和受控中采用许多精心设计的实验 实验室环境。这些实验旨在阐明环境，生态和遗传因素的作用 在使用两个医学重要载体的夏威夷种群的夏威夷人群中，culex的蚊子多样性多样性 Quinquefasciatus和Ades bopotus。此外，它将调查这种多样性对 登革热传播，这是夏威夷最相关的蚊子传播病原体。这项工作的预期结果 包括一个更好的模型，用于组装蚊子微生物群及其对病原体的累积影响 传播。最终，预计这项研究将通过告知 开发基于微生物的新型策略，以减轻蚊子传播的公共卫生问题。