Archaea in African Americans with polyps

患有息肉的非洲裔美国人中的古细菌

基本信息

批准号：
10575726
负责人：
ECE A. MUTLU
金额：
$ 8万
依托单位：
UNIVERSITY OF ILLINOIS AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-12-12 至 2024-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10575726
关键词：
Adenomatous Polyps African American population Age Alcohol consumption Archaea Bacteria Bile Acids Biological Assay Body mass index Colon Colon Carcinoma Colorectal Cancer Constipation Control Groups Data Data Set Diet Environment Environmental Risk Factor Ethnic Population Face Feces Formates Funding Gases Genetic Genomics Growth Hong Kong Human Hydrogen Individual Inflammatory Bowel Diseases Ingestion Intestines Irritable Bowel Syndrome Knowledge Lactulose Metabolism Metadata Metagenomics Methane Methanobacteria Methanobrevibacter Not Hispanic or Latino Nucleic Acids Pathway interactions Patients Polyps Predisposition Production Protocols documentation Rest Sampling Shotguns Smoking Spain Specimen Structure United States National Institutes of Health Validation cancer health disparity cell envelope cohort colon bacteria colorectal cancer risk comparison control gut bacteria gut microbiome high risk metagenome metagenomic sequencing microbial microbiome prevent racial disparity racial population sample collection sex social social determinants stool sample

项目摘要

It is well-known that African Americans (AAs) face a higher risk and burden of colorectal cancer (CRC), but which genetic pathways or environmental/social determinants add to the risk of colorectal cancer are not well-known. Our overall working hypothesis is that the archaea in the human gut lumen are different in AAs compared to Non-Hispanic Whites (NHWs) and this difference (either in the numbers of archaea, types and/or function) contributes to the racial disparities observed in CRC. With this hypothesis, the study will use an existing sample set of AAs and NHWs with and without polyps. The first aim will determine M.Smithii and other archaea in AAs with and without polyps vs. NHWs with and without polyps using qPCR, amplicon and metagenomic sequencing. The second aim will examine genomic differences of M.smithii isolates from fecal samples of AAs with and without polyps vs NHWs with and without polyps. This will be the first study to examine archaea in the gut microbiome of AAs; and look at archaea diversity and functional potential in AAs with and without polyps and appropriate NHW controls, to understand CRC disparities in AAs. If differences are found similar to our preliminary data, manipulation of the archaea in AAs at risk for CRC can be considered, after further studies in larger cohorts.

众所周知，非洲裔美国人 (AA) 面临着更高的结直肠癌风险和负担癌症 (CRC)，但遗传途径或环境/社会决定因素会增加风险结直肠癌的发病率尚不为人所知。我们的总体工作假设是古细菌与非西班牙裔白人 (NHW) 相比，AA 的人类肠道管腔有所不同，这差异（古菌数量、类型和/或功能）导致种族差异 CRC 中观察到的差异。根据这一假设，本研究将使用现有的样本集有或没有息肉的 AA 和 NHW。第一个目标将确定 M.Smithii 和其他人使用 qPCR 比较有和没有息肉的 AA 中的古细菌与有和没有息肉的 NHW 中的古细菌，扩增子和宏基因组测序。第二个目标将检查基因组差异 M.smithii 从粪便样本中分离出带或不带息肉的 AA 与带或不带息肉的 NHW 息肉。这将是第一项检查 AA 肠道微生物组中古细菌的研究；看看有或没有息肉的 AA 中的古菌多样性和功能潜力以及适当的 NHW 控制，以了解 AA 中的 CRC 差异。如果发现与我们初步的差异类似数据显示，经过进一步研究，可以考虑对有 CRC 风险的 AA 中的古菌进行操作在更大的队列中。